Method for breeding self-compatible potatoes

Abstract

Disclosed is a method for breeding self-compatible potatoes, including the following steps: (1) selecting a self-compatible potato variety material and referring to it as PG6359, and cloning the S-RNase gene of PG6359 through the transcriptome sequencing method; and (2) obtaining two full-length sequences of the S-RNase gene from the cloned S-RNase gene in step (1) and referring to them as S s11 and S s12 respectively, and after carrying out an artificial self-pollination for the variety material PG6359, selecting the variety material having the genotype of S s11 S s11 from the offspring as the female parent, and selecting a self-incompatible material as the male parent, and then obtaining a self-compatible F 1 generation by hybridization. The invention overcomes the self-incompatibility of diploid potatoes, and does not require the introduction of any wild potato gene fragments, thereby avoiding linkage drag, and providing a basis for the rapid creation of a diploid potato inbred line.

What is claimed is: 
     
         1 . A self-compatible diploid potato plant or part thereof, comprising a S s11  S-RNase allele encoding a transcript 95% or more identical to SEQ ID NO:1, and lacking a S s12  S-RNase allele encoding a transcript 95% or more identical to SEQ ID NO:2. 
     
     
         2 . The self-compatible diploid potato plant or part thereof of  claim 1 , wherein the plant is an inbred. 
     
     
         3 . The self-compatible diploid potato plant or part thereof of  claim 1 , wherein the plant is homozygous for the S s11  S-RNase allele. 
     
     
         4 . The self-compatible diploid potato plant or part thereof of  claim 1 , wherein the S s11  S-RNase allele encodes a transcript 98% or more identical to SEQ ID NO:1. 
     
     
         5 . The self-compatible diploid potato plant or part thereof of  claim 1 , wherein the S s11  S-RNase allele encodes a transcript 99% or more identical to SEQ ID NO:1. 
     
     
         6 . The self-compatible diploid potato plant or part thereof of  claim 1 , wherein the S s11  S-RNase allele encodes a transcript 100% identical to SEQ ID NO:1. 
     
     
         7 . The self-compatible diploid potato plant or part thereof of  claim 1 , wherein the S s11  S-RNase allele is from potato variety PG6359. 
     
     
         8 . The self-compatible diploid potato plant or part thereof of  claim 1 , wherein the S s12  S-RNase allele encodes a transcript 98% or more identical to SEQ ID NO:2. 
     
     
         9 . The self-compatible diploid potato plant or part thereof of  claim 1 , wherein the S s12  S-RNase allele encodes a transcript 99% or more identical to SEQ ID NO:2. 
     
     
         10 . The self-compatible diploid potato plant or part thereof of  claim 1 , wherein the S s12  S-RNase allele encodes a transcript 100% identical to SEQ ID NO:2. 
     
     
         11 . The self-compatible diploid potato plant or part thereof of  claim 6 , wherein the S s12  S-RNase allele encodes a transcript 100% identical to SEQ ID NO:2. 
     
     
         12 . The self-compatible diploid potato plant or part thereof of  claim 1 , wherein the part thereof is a tuber or seed. 
     
     
         13 . The self-compatible diploid potato plant or part thereof of  claim 11 , wherein the part thereof is a tuber or seed. 
     
     
         14 . A food product comprising materials from tuber of the self-compatible diploid potato plant of  claim 1 . 
     
     
         15 . The food product of  claim 14 , wherein the food product is selected from the group consisting of fresh whole potatoes, French fries, potato chips, dehydrated potato material, potato flakes and potato granules. 
     
     
         16 . A method for manufacturing a commercial plant product, which comprises: obtaining a tuber, a tuber part of the self-compatible diploid potato plant or part thereof of  claim 1 , manufacturing the commercial plant product, wherein the plant product is selected from the group consisting of fresh whole potatoes, French fries, potato chips, dehydrated potato materials, potato flakes, and potato granules. 
     
     
         17 . A complementary DNA molecule comprising a sequence 95% or more identical to SEQ ID NO:1. 
     
     
         18 . The complementary DNA molecule of  claim 17 , comprising the sequence of SEQ ID NO:1. 
     
     
         19 . A method of overcoming self-incompatibility of diploid potato, comprising: (i) crossing a first self-compatible diploid potato plant having a S s11 S s11  genotype at the S-RNase locus with a second self-incompatible diploid potato plant; (ii) introgressing the S s11 S s11  genotype to the second diploid potato plant to convert the second diploid potato plant from self-incompatible to self-compatible.