Nano-ligand for promoting cell adhesion and differentiation of stem cells and method of promoting cell adhesion and differentiation of stem cells by using the same

Abstract

The present invention relates to a nano-ligand for promoting cell adhesion and differentiation of stem cells and a method of promoting cell adhesion and differentiation of stem cells by using the nano-ligand, and the method of promoting cell adhesion and differentiation of stem cells according to the present invention may temporally and spatially, and reversibly control nano-ligand sliding by applying a magnetic field to a substrate including the nano-ligands, and efficiently control stem cell adhesion and differentiation ex vivo or in vivo through the magnetic-field based on spatiotemporal control.

What is claimed is: 
     
         1 . A nano-ligand for promoting cell adhesion and differentiation of stem cells, the nano-ligand comprising:
 a core including magnetic nano-particles;   a coating layer provided to surround the core and including an integrin-binding ligand peptide; and   a linker provided between the core and the coating layer,   wherein the integrin-binding ligand peptide is negatively charged.   
     
     
         2 . The nano-ligand of  claim 1 , wherein a surface of the magnetic nanoparticle is coated with silica. 
     
     
         3 . The nano-ligand of  claim 1 , wherein the nano-ligand has a structure in which the core and the coating layer are connected through the linker, and
 the linker is a polyethylene glycol (PEG) linker.   
     
     
         4 . The nano-ligand of  claim 1 , wherein a diameter of the nano-ligand is 30 nm to 60 nm, and a diameter of the magnetic nanoparticle is 5 nm to 30 nm. 
     
     
         5 . The nano-ligand of  claim 1 , wherein the integrin-binding ligand peptide includes a negatively charged thiolated integrin-binding ligand peptide, and
 a surface of the nano-ligand is provided in a negatively charged form.   
     
     
         6 . A method of preparing the nano-ligand for promoting cell adhesion and differentiation of stem cells of  claim 1 , the method comprising:
 preparing a core including magnetic nano-particles;   preparing a core coupled with a linker by mixing the core and a first suspension including a first linker; and   mixing the core coupled with the linker and a second suspension including an integrin-binding ligand peptide (RGD).