Antibody for porcine reproductive and respiratory syndrome virus and uses thereof

Abstract

The instant disclosure discloses an antibody or antigen-binding fragment thereof binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and uses of such antibody or antigen-binding fragment thereof to create immunoassay methods or devices for PRRSV detection.

What is claimed is: 
     
         1 . An antibody or antigen-binding fragment thereof binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the antibody or antigen-binding fragment thereof comprising:
 a heavy chain variable domain comprising a heavy chain complementarity-determining region 1 (CDR-H1) containing a sequence of SEQ ID NO: 1, a CDR-H2 containing a sequence of SEQ ID NO: 2, and a CDR-H3 containing a sequence of SEQ ID NO: 3; and   a light chain variable domain comprising a light chain complementarity-determining region 1 (CDR-L1) containing a sequence of SEQ ID NO: 4, a CDR-L2 containing a sequence of Lys-Ala-Ser, and a CDR-L3 containing a sequence of SEQ ID NO: 5.   
     
     
         2 . The antibody or antigen-binding fragment thereof of  claim 1 , wherein the heavy chain variable domain comprises a sequence of SEQ ID NO: 6. 
     
     
         3 . The antibody or antigen-binding fragment thereof of  claim 1 , wherein the light chain variable domain comprises a sequence of SEQ ID NO: 7. 
     
     
         4 . An isolated nucleic acid encoding the antibody or antigen-binding fragment thereof of  claim 1 , wherein the isolated nucleic acid encoding the antibody or antigen-binding fragment thereof comprises a first fragment encoding the heavy chain variable domain and a second fragment encoding the light chain variable domain. 
     
     
         5 . The isolated nucleic acid encoding the antibody or antigen-binding fragment thereof of  claim 4 , wherein the first fragment encoding the heavy chain variable domain further comprises a sequence of SEQ ID NO: 8. 
     
     
         6 . The isolated nucleic acid encoding the antibody or antigen-binding fragment thereof of  claim 4 , wherein the second fragment encoding the light chain variable domain further comprises a sequence of SEQ ID NO: 9. 
     
     
         7 . A vector comprising the isolated nucleic acid of  claim 4 . 
     
     
         8 . A host cell comprising the vector of  claim 7 . 
     
     
         9 . A method for producing an antibody or antigen-binding fragment thereof binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the method comprising:
 (a) culturing the host cell of  claim 8  under conditions suitable for expressing the antibody or antigen-binding fragment thereof; and   (b) recovering the antibody or antigen-binding fragment thereof,   wherein the antibody or antigen-binding fragment thereof comprises a heavy chain variable domain and a light chain variable domain.   
     
     
         10 . The method for producing an antibody or antigen-binding fragment thereof binding to PRRSV of  claim 9 , wherein the heavy chain variable domain further comprises a sequence of SEQ ID NO: 6. 
     
     
         11 . The method for producing an antibody or antigen-binding fragment thereof binding to PRRSV of  claim 9 , wherein the light chain variable domain further comprises a sequence of SEQ ID NO: 7. 
     
     
         12 . A method for detecting Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the method comprising contacting a sample with the antibody or antigen-binding fragment thereof of  claim 1 . 
     
     
         13 . The method of  claim 12 , wherein the heavy chain variable domain further comprises a sequence of SEQ ID NO: 6. 
     
     
         14 . The method of  claim 12 , wherein the light chain variable domain further comprises a sequence of SEQ ID NO: 7. 
     
     
         15 . A biological field-effect transistor (Bio-FET), comprising:
 a transistor region; and   a detecting region, wherein the detecting region comprises a detecting surface that is functionalized with an antibody or antigen-binding fragment thereof binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), wherein the antibody or antigen-binding fragment thereof comprises:
 a heavy chain variable domain comprising a heavy chain complementarity-determining region 1 (CDR-H1) containing a sequence of SEQ ID NO: 1, a CDR-H2 containing a sequence of SEQ ID NO: 2, and a CDR-H3 containing a sequence of SEQ ID NO: 3; and 
 a light chain variable domain comprising a light chain complementarity-determining region 1 (CDR-L1) containing a sequence of SEQ ID NO: 4, a CDR-L2 containing a sequence of Lys-Ala-Ser, and a CDR-L3 containing a sequence of SEQ ID NO: 5. 
   
     
     
         16 . The Bio-FET of  claim 15 , wherein the heavy chain variable domain further comprises a sequence of SEQ ID NO: 6. 
     
     
         17 . The Bio-FET of  claim 15 , wherein the light chain variable domain further comprises a sequence of SEQ ID NO: 7. 
     
     
         18 . A method for detecting Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by using a biological field-effect transistor (Bio-FET), the method comprising:
 (a) contacting a sample with an antibody or antigen-binding fragment thereof immobilized on a detecting surface of the Bio-FET, wherein the antibody or antigen-binding fragment thereof comprises:
 a heavy chain variable domain comprising a heavy chain complementarity-determining region 1 (CDR-H1) containing a sequence of SEQ ID NO: 1, a CDR-H2 containing a sequence of SEQ ID NO: 2, and a CDR-H3 containing a sequence of SEQ ID NO: 3; and 
 a light chain variable domain comprising a CDR-L1 containing a sequence of SEQ ID NO: 4, a CDR-L2 containing a sequence of Lys-Ala-Ser, and a CDR-L3 containing a sequence of SEQ ID NO: 5, 
 wherein the antibody or antigen-binding fragment thereof binds to PRRSV; and 
   (b) analyzing an electric signal obtained from the Bio-FET.   
     
     
         19 . The method of  claim 18 , wherein the heavy chain variable domain further comprises a sequence of SEQ ID NO: 6. 
     
     
         20 . The method of  claim 18 , wherein the light chain variable domain further comprises a sequence of SEQ ID NO: 7.