US2023286920A1PendingUtilityA1

Cyclooctynes for click chemistry

Assignee: MASSACHUSETTS INST TECHNOLOGYPriority: May 27, 2021Filed: May 1, 2023Published: Sep 14, 2023
Est. expiryMay 27, 2041(~14.8 yrs left)· nominal 20-yr term from priority
C07D 221/16C07D 231/54C07D 471/04C07D 249/16C07C 13/547C07C 2603/36
70
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Claims

Abstract

Provided herein are dibenzocyclooctyne compounds useful as reagents in 1,3-dipolar cycloaddition reactions, and methods for their preparation.

Claims

exact text as granted — not AI-modified
1 . A compound of formula (I): 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable salt thereof, 
         wherein: 
         R 1  and R 2  are, independently for each occurrence, F, Cl, Br, I, OTf, B(OH) 2 , CN, NHR 5 , NHS(O) 2 R 5 , OR 5 , OS(O) 2 R 5 , SR 5 , —CF 3 , —C(O)OC 1-6  alkyl, —C(O)N(C 1-6  alkyl) 2 , —C(O)R 5 , S(O) 2 R 5 , NO 2 , —C 1-6  alkyl, —C 1-6  alkenyl, or 5- to 10-membered heteroaryl substituted with 1, 2, 3, 4, or 5 R 6  groups, optionally wherein the alkyl and alkenyl are substituted with one or more R 5  groups; 
         R 3  and R 4  are, independently for each occurrence, H or —C 1-6  alkyl; 
         R 5  is selected from H, —C 1-6 -alkyl, —CF 3 , —C(O)OC 1-6  alkyl, or —C(O)N(C 1-6  alkyl) 2 ; 
         R 6  is selected from H, F, Cl, Br, I, OTf, CN, NH 2 , OR 5 , SR 5 , —CF 3 ; —C(O)R 5 , —C(O)OC 1-6  alkyl, NO 2 , or —C 1-6  alkyl; 
         n is 0, 1, or 2; 
         p is 0, 1, or 2; 
         X 1  is N or CH; and 
         X 2  is N +  or C; 
         provided that when X 1  is N, X 2  is C; or when X 1  is CH, X 2  is N + . 
       
     
     
         2 . The compound of  claim 1 , having a structure according to formula (Ia): 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable salt thereof, 
         wherein: 
         R 1  and R 2  are, independently for each occurrence, F, Cl, Br, I, OTf, B(OH) 2 , CN, NHR 5 , NHS(O) 2 R 5 , OR 5 , OS(O) 2 R 5 , SR 5 , —CF 3 , —C(O)OC 1-6  alkyl, —C(O)N(C 1-6  alkyl) 2 , —C(O)R 5 , S(O) 2 R 5 , NO 2 , —C 1-6  alkyl, —C 1-6  alkenyl, or 5- to 10-membered heteroaryl substituted with 1, 2, 3, 4, or 5 R 6  groups, optionally wherein the alkyl and alkenyl are substituted with one or more R 5  group; 
         R 3  and R 4  are, independently for each occurrence, H or —C 1-6  alkyl; 
         R 5  is selected from H, —C 1-6 -alkyl, —CF 3 , —C(O)OC 1-6  alkyl, or —C(O)N(C 1-6  alkyl) 2 ; 
         R 6  is selected from H, F, Cl, Br, I, OTf, CN, NH 2 , OR 5 , SR 5 , —CF 3 , —C(O)R 5 , —C(O)OC 1-6  alkyl, NO 2 , —C 1-6  alkyl; 
         n is 0, 1, or 2; and 
         p is 0, 1, or 2. 
       
     
     
         3 . The compound of  claim 2 , wherein R 3  and R 4  are H. 
     
     
         4 . The compound of  claim 2 , wherein R 1  and R 2  are F, Cl, Br, I, OTf, or B(OH) 2 . 
     
     
         5 . The compound of  claim 2 , wherein R 1  and R 2  are Cl. 
     
     
         6 . The compound of  claim 2 , wherein n is 1 and p is 0. 
     
     
         7 . The compound of  claim 1 , having a structure according to formula (Ib): 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable salt thereof, 
         wherein R 1  is, independently for each occurrence, F, Cl, Br, I, OTf, B(OH) 2  or —C 1-6 -alkyl; and 
         n is 0, 1, or 2. 
       
     
     
         8 . The compound of  claim 1 , wherein the compound of formula (I) is: 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable salt thereof. 
       
     
     
         9 . A process of making a compound of formula (II): 
       
         
           
           
               
               
           
         
         or a pharmaceutically accepted salt thereof, 
         wherein: 
         R 1  and R 2  are, independently for each occurrence, F, Cl, Br, I, OTf, B(OH) 2 , CN, NHR 5 , NHS(O) 2 R 5 , OR 5 , OS(O) 2 R 5 , SR 5 , —CF 3 , —C(O)OC 1-6  alkyl, —C(O)N(C 1-6  alkyl) 2 , —C(O)R 5 , S(O) 2 R 5 , NO 2 , —C 1-6  alkyl, —C 1-6  alkenyl, or 5- to 10-membered heteroaryl substituted with 1, 2, 3, 4, or 5 R 6  groups, optionally wherein the alkyl and alkenyl are substituted with one or more R 5  group; 
         R 3  and R 4  are, independently for each occurrence, H or —C 1-6  alkyl; 
         R 5  is selected from H, —C 1-6 -alkyl, —CF 3 ; —C(O)OC 1-6  alkyl, or —C(O)N(C 1-6  alkyl) 2 ; 
         R 6  is selected from H, F, Cl, Br, I, OTf, CN, NH 2 , OR 5 , SR 5 , —CF 3 , —C(O)R 5 , —C(O)OC 1-6  alkyl, NO 2 , —C 1-6  alkyl; 
         n is 0, 1, or 2; 
         p is 0, 1, or 2; and 
         X is CH or N; 
         wherein the process comprises: 
         (A) combining a compound of formula (III): 
       
       
         
           
           
               
               
           
         
         with a compound of formula (IV): 
       
       
         
           
           
               
               
           
         
         and a non-nucleophilic base to provide the compound of formula (V): 
       
       
         
           
           
               
               
           
         
       
       and
 (B) combining the compound of formula (V) with a carbodiimide to provide the compound of formula (II). 
 
     
     
         10 . The process of  claim 9 , wherein the compound of formula (II), has a structure according to formula (IIa): 
       
         
           
           
               
               
           
         
         wherein R 1  is, independently for each occurrence, F, Cl, Br, I, OTf, B(OH) 2  or —C 1-6 -alkyl; 
         n is 0, 1, or 2; and 
         X is CH or N. 
       
     
     
         11 . The process of  claim 9 , wherein the compound of formula (II), has a structure selected from the group consisting of: 
       
         
           
           
               
               
           
         
         wherein R 1  is Cl; and 
         n is 0 or 1. 
       
     
     
         12 . The process of  claim 9 , wherein the compound of formula (II), has a structure selected from the group consisting of: 
       
         
           
           
               
               
           
         
       
     
     
         13 . The process of  claim 9 , wherein the non-nucleophilic base is an alkyl lithium. 
     
     
         14 . The process of  claim 9 , wherein the non-nucleophilic base is lithium bis(trimethylsilyl)amide. 
     
     
         15 . The process of  claim 9 , wherein the carbodiimide is 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). 
     
     
         16 . The process of  claim 9 , wherein step (B) comprises an alkylidene carbene rearrangement. 
     
     
         17 . A process of making a compound of formula (VIa): 
       
         
           
           
               
               
           
         
         or regioisomer thereof, 
         wherein: 
         R 1  and R 2  are, independently for each occurrence, F, Cl, Br, I, OTf, B(OH) 2 , CN, NHR 5 , NHS(O) 2 R 5 , OR 5 , OS(O) 2 R 5 , SR 5 , —CF 3 , —C(O)OC 1-6  alkyl, —C(O)N(C 1-6  alkyl) 2 , —C(O)R 5 , S(O) 2 R 5 , NO 2 , —C 1-6  alkyl, —C 1-6  alkenyl, or 5- to 10-membered heteroaryl substituted with 1, 2, 3, 4, or 5 R 6  groups, optionally wherein the alkyl and alkenyl are substituted with one or more R 5  groups; 
         R 3  and R 4  are, independently for each occurrence, H or —C 1-6  alkyl; 
         R 5  is selected from H, —C 1-6 -alkyl, —CF 3 ; —C(O)OC 1-6  alkyl, or —C(O)N(C 1-6  alkyl) 2 ; 
         R 6  is selected from H, F, Cl, Br, I, OTf, CN, NH 2 , OR 5 , SR 5 , —CF 3 , —C(O)R 5 , —C(O)OC 1-6  alkyl, NO 2 , —C 1-6  alkyl; 
         R 7  is C 1-6  alkyl optionally substituted with one or more C 6-10  aryl or 5- to 10-membered heteroaryl; 
         n is 0, 1, or 2; 
         p is 0, 1, or 2; 
         X is CH or N; and 
         Y is O or NH; 
         wherein the process comprises combining a compound of formula (II): 
       
       
         
           
           
               
               
           
         
         with a compound of formula (VIIa): 
       
       
         
           
           
               
               
           
         
         to provide the compound of formula (VIa). 
       
     
     
         18 . The process of  claim 17 , wherein the compound of formula (VIIa) is selected from the group consisting of: 
       
         
           
           
               
               
           
         
       
     
     
         19 . The process of  claim 17 , wherein the compound of formula (II) has a structure according to formula (IIa): 
       
         
           
           
               
               
           
         
         wherein: 
         R 1  is, independently for each occurrence, F, Cl, Br, I, OTf, B(OH) 2  or —C 1-6 -alkyl; 
         n is 0, 1, or 2; and 
         X is CH or N. 
       
     
     
         20 . The process of  claim 17 , wherein the compound of formula (II), has a structure selected from the group consisting of: 
       
         
           
           
               
               
           
         
         wherein R 1  is Cl; and 
         wherein n is 0 or 1. 
       
     
     
         21 . The process of  claim 17 , wherein the compound of formula (II), has a structure selected from the group consisting of: 
       
         
           
           
               
               
           
         
       
     
     
         22 . A process of making a compound of formula (VIb): 
       
         
           
           
               
               
           
         
         or a regioisomer thereof, 
         wherein: 
         R 1  and R 2  are, independently for each occurrence, F, Cl, Br, I, OTf, B(OH) 2 , CN, NHR 5 , NHS(O) 2 R 5 , OR 5 , OS(O) 2 R 5 , SR 5 , —CF 3 , —C(O)OC 1-6  alkyl, —C(O)N(C 1-6  alkyl) 2 , —C(O)R 5 , S(O) 2 R 5 , NO 2 , —C 1-6  alkyl, —C 1-6  alkenyl, or 5- to 10-membered heteroaryl substituted with 1, 2, 3, 4, or 5 R 6  groups, wherein the alkyl and alkenyl are optionally substituted with one or more R 5  groups; 
         R 3  and R 4  are, independently for each occurrence, H or —C 1-6  alkyl; 
         R 5  is selected from H, —C 1-6 -alkyl, —CF 3 ; —C(O)OC 1-6  alkyl, or —C(O)N(C 1-6  alkyl) 2 ; 
         R 6  is selected from H, F, Cl, Br, I, OTf, CN, NH 2 , OR 5 , SR 5 , —CF 3 , —C(O)R 5 , —C(O)OC 1-6  alkyl, NO 2 , —C 1-6  alkyl; 
         R 7  is C 1-6  alkyl optionally substituted with one or more C 6-10  aryl or 5- to 10-membered heteroaryl; 
         n is 0, 1, or 2; 
         p is 0, 1, or 2; 
         X is CH or N; and 
         Y is O or NH; 
         wherein the process comprises combining a compound of formula (II): 
       
       
         
           
           
               
               
           
         
         with a compound of formula (VIIb): 
       
       
         
           
           
               
               
           
         
         to provide the compound of formula (VIb). 
       
     
     
         23 . The process of  claim 22 , wherein the compound of formula (VIIb) is: 
       
         
           
           
               
               
           
         
       
     
     
         24 . The process of  claim 22 , wherein the compound of formula (II) has a structure according to formula (IIa): 
       
         
           
           
               
               
           
         
         wherein: 
         R 1  is, independently for each occurrence, F, Cl, Br, I, OTf, B(OH) 2  or —C 1-6 -alkyl; 
         n is 0, 1, or 2; and 
         X is CH or N. 
       
     
     
         25 . The process of  claim 22 , wherein the compound of formula (II), has a structure selected from the group consisting of: 
       
         
           
           
               
               
           
         
         wherein R 1  is Cl; and 
         wherein n is 0 or 1. 
       
     
     
         26 . The process of  claim 22 , wherein the compound of formula (II), has a structure selected from the group consisting of:

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