Artificial Cells and Delivery Devices for Use in Tissue Engineering, and Related Methods

Abstract

Provided herein is a drug delivery device and composition, such as a particle, comprising conditioned medium. Also provided herein is a method of preparing polymeric particles for release of conditioned medium. Further, a tissue growth scaffold comprising particles for release of conditioned medium is provided.

We claim: 
     
         1 . A composition comprising a first particle comprising conditioned medium from a cell culture within the first particle, and having a first release profile of the conditioned medium. 
     
     
         2 . The composition of  claim 1 , further comprising a second particle different from the first particle, comprising conditioned medium from a cell culture within the second particle and having a second release profile of the conditioned medium different from the first release profile, thereby producing an extended release profile, releasing effective amounts of the conditioned medium beyond the first release profile, wherein the conditioned medium of the first and second particles are the same or different. 
     
     
         3 . The composition of  claim 2 , further comprising one or more additional particles, each independently comprising conditioned medium from a cell culture within the particle and having a release profile of the conditioned medium different from the first and second release profile, thereby further extending release of effective amounts of the conditioned medium beyond the first and second release profile, wherein the conditioned medium of the first, second, and one or more additional particles are the same or different. 
     
     
         4 . The composition of  claim 1 , wherein the first particle comprises a polymer material, a ceramic material, or a material capable of osmotic release. 
     
     
         5 . The composition of  claim 1 , wherein the conditioned medium is medium from a culture of a stem cell, a progenitor cell, an immune cell, a secretory cell, or an islet cell. 
     
     
         6 . The composition of  claim 5 , wherein the conditioned medium is medium from a culture of a mesenchymal stem cell or a macrophage. 
     
     
         7 . The composition of  claim 1 , wherein the first particle comprises a polyester or polyester-containing copolymer. 
     
     
         8 . The composition of  claim 7 , wherein the polyester or polyester-containing copolymer comprises a poly(lactic-co-glycolic) acid (PLGA); a poly(lactic acid) (PLA); a poly(trimethylene carbonate) (PTMC); poly(caprolactone) (PCL); a poly(glycolic acid) (PGA); or a poly(glycolide-co-trimethylene carbonate) (PGTMC). 
     
     
         9 . The composition of  claim 1 , further comprising a biocompatible polymer. 
     
     
         10 . The composition of  claim 9 , wherein the biocompatible polymer is a bioerodible polymer. 
     
     
         11 . The composition of  claim 10 , wherein the biocompatible, bioerodible polymer is a poly(ester urethane) urea (PEUU); poly(ether ester urethane)urea (PEEUU); poly(ester carbonate)urethane urea (PECUU); poly(carbonate)urethane urea (PCUU); a polyurethane; a polyester; a polymer comprising monomers derived from alpha-hydroxy acids; a polymer comprising monomers derived from esters; a polymer comprising monomers derived from lactones; and/or a polymer comprising monomers derived from carbonates. 
     
     
         12 . The composition of  claim 11 , wherein the alpha-hydroxy acids comprise polylactide, poly(lactide-co-glycolide), poly(L-lactide-co-caprolactone), polyglycolic acid, poly(D,L-lactide-co-glycolide), and/or poly(L-lactide-co-D,L-lactide). 
     
     
         13 . The composition of  claim 11 , wherein the ester comprises polyhydroxybutyrate, polyhydroxyvalerate, polydioxanone, and/or polyglactin. 
     
     
         14 . The composition of  claim 11 , wherein the lactone comprises polycaprolactone. 
     
     
         15 . The composition of  claim 11 , wherein the carbonate comprises polycarbonate, polyglyconate, poly(glycolide-co-trimethylene carbonate), or poly(glycolide-co-trimethylene carbonate-co-dioxanone). 
     
     
         16 . The composition of  claim 1 , further comprising a growth factor, cytokine, chemoattractant, and/or inflammation regulator within the first particle. 
     
     
         17 . The composition of  claim 1 , further comprising IL-2, IL-12, and/or IFN-γ within the first particle. 
     
     
         18 . The composition of  claim 1 , further comprising a non-steroidal anti-inflammatory drug within the first particle. 
     
     
         19 . The composition of  claim 1 , further comprising a composition that promotes angiogenesis within the first particle. 
     
     
         20 . The composition of  claim 1 , further comprising an anti-thrombogenic composition within the first particle.