Method, process, composition and kit for measuring concentration of molecules dissolved in the continuous phase of a colloid

Abstract

A method for measuring a concentration of molecules, characterized in that the method measures the concentration of molecules dissolved in a continuous phase of a colloid and includes obtaining a test sample by mixing a number of molecules with a volume of colloid, obtaining a control sample by mixing a number of molecules with a volume of a composition comprising a particle-free solution extracted from a fraction of the continuous phase of same colloid used in the obtaining the test sample, so that a value of the concentration of molecules in the mixture is equal to the value of the concentration of molecules in the test sample obtained in the obtaining the test sample, and submitting the test and the control samples obtained in the obtaining the test sample and obtaining the control sample to a process in order to concentrate the particles of the test sample.

1 . A process for obtaining a particle-free solution composition, characterized in that said process comprises the steps of:
 a) concentrating particles contained in a colloid sample for obtaining a continuous phase of the colloid;   b) recovering a fraction of the continuous phase of the colloid;   c) measuring in the recovered fraction of step b) a parameter proportional to the concentration of particles of the colloid; and   d) storing a volume of a particle-free solution which is equivalent to the continuous phase of the colloid sample used in step a).   
     
     
         2 . A particle-free solution composition, characterized in that said particle-free solution is extracted from a colloid and is equivalent to a fraction of a continuous phase of the same colloid. 
     
     
         3 . A Kit for measuring the concentration of molecules dissolved in the continuous phase of a colloid, characterized in that said kit comprises:
 a volume of the colloid; and   a volume of a composition comprising a particle-free solution extracted from a fraction of the continuous phase of the same colloid.