Coating film having compatibility with biological substance containing block copolymer

Abstract

The invention provides a composition for forming a coating film having compatibility with a biological substance which comprises a block copolymer having unit structures represented by the formula (1) and the formula (2):(wherein R1 to R3, U1 and U2 each independently represent a hydrogen atom or an alkyl group having 1 to 5 carbon atoms, X1 and X2 represent an alkylene group having 1 to 5 carbon atoms, and n1 represents an integer of 1 to 10), and a solvent. The invention also provides a coating film using the same, a substrate for cell culture using the same, a method for producing the substrate for cell culture, and a method for producing a cell aggregate.

1 . A composition for forming a coating film having compatibility with a biological substance which comprises a block copolymer having unit structures represented by the formula (1) and the formula (2): 
       
         
           
           
               
               
           
         
       
       wherein R 1  to R 3 , U 1  and U 2  each independently represent a hydrogen atom or an alkyl group having 1 to 5 carbon atoms, X 1  and X 2  each independently represent an alkylene group having 1 to 5 carbon atoms, and n1 represents an integer of 1 to 10, and a solvent. 
     
     
         2 . The composition according to  claim 1 , wherein the solvent contains water or an alcohol. 
     
     
         3 . The composition according to  claim 1 , wherein the compatibility with a biological substance is an ability to suppress adhesion of a protein. 
     
     
         4 . The composition according to  claim 1 , wherein the compatibility with a biological substance is for forming a base film for cell culture. 
     
     
         5 . The composition according to  claim 4 , which is used for forming a base film for cell culture to obtain a cell aggregate by adhering and then peeling cells. 
     
     
         6 . A coating film having compatibility with a biological substance, which is a coating film of the composition for forming a coating film having compatibility with a biological substance according to  claim 1 . 
     
     
         7 . A substrate for cell culture, which comprises the coating film having compatibility with a biological substance according to  claim 6  at least at a part of a surface of a substrate having an ability to suppress adhesion of a biological substance. 
     
     
         8 . The substrate for cell culture according to  claim 7 , wherein the substrate having an ability to suppress adhesion of a biological substance is a substrate provided with a coating film containing a copolymer which contains a recurring unit containing a group represented by the following formula (a) and a recurring unit containing a group represented by the following formula (b): 
       
         
           
           
               
               
           
         
         wherein U a1 , U a2 , U b1 , U b2  and U b3  each independently represent a hydrogen atom or an alkyl group having 1 to 5 carbon atoms, An −  represents an anion selected from the group consisting of a halide ion, an inorganic acid ion, a hydroxide ion and an isothiocyanate ion 
       
       on at least a part of a surface thereof. 
     
     
         9 . A method for producing a substrate for cell culture which comprises a step of applying
 a composition for forming a coating film having compatibility with a biological substance containing a block copolymer having unit structures represented by the formula (1) and the formula (2):   
       
         
           
           
               
               
           
         
       
       wherein R 1  to R 3 , U 1  and U 2  each independently represent a hydrogen atom or an alkyl group having 1 to 5 carbon atoms, X 1  and X 2  each independently represent an alkylene group having 1 to 5 carbon atoms, and n1 represents an integer of 1 to 10, and
 a solvent 
 
       to a substrate having an ability to suppress adhesion of a biological substance, and then, a step of drying the same. 
     
     
         10 . A method for producing a cell aggregate which comprises a step of providing a base film for cell culture comprising a block copolymer having unit structures represented by the formula (1) and the formula (2): 
       
         
           
           
               
               
           
         
         wherein R 1  to R 3 , U 1  and U 2  each independently represent a hydrogen atom or an alkyl group having 1 to 5 carbon atoms, X 1  and X 2  each independently represent an alkylene group having 1 to 5 carbon atoms, and n1 represents an integer of 1 to 10, 
       
       onto at least a part of a surface of a substrate, and a step of seeding cells on the base film.