Method for judging risk of suffering kidney cancer

Abstract

The invention relates to a method for judging risk of suffering kidney cancer. The method includes detecting a PSMB9 protein concentration value from a test sample. And a risk factor value is obtained after processing the PSMB9 protein concentration value with an age. When the score of the risk factor value is greater than a defined threshold value of the risk factor threshold value, then the test sample is classified as a high risk sample of a of potential kidney cancer patient.

What is claimed is: 
     
         1 . A method of judging a risk of suffering kidney cancer, includes the steps of:
 obtaining a test sample;   detecting a PSMB9 protein in the test sample to obtain a PSMB9 protein concentration value, in which the PSMB9 protein has the amino acid sequence of SEQ ID NO: 1;   the PSMB9 protein concentration value to obtain a value of a risk factor;   comparing the value of the risk factor with a threshold value of the risk factor; and   when the value of the risk factor is greater than the threshold value of the risk factor, judge that the test sample has a high risk of suffering kidney cancer;   wherein the threshold value of the risk factor is 0.037 (fmol PSMB9/μg urine protein).   
     
     
         2 . A method of judging a risk of suffering kidney cancer, includes the steps of:
 obtaining a test sample;   detecting a PSMB9 protein in the test sample to obtain a PSMB9 protein concentration value, in which the PSMB9 protein has the amino acid sequence of SEQ ID NO: 1;   multiplying the PSMB9 protein concentration value by an age to obtain a value of a first risk factor;   comparing the value of the first risk factor with a threshold value of the first risk factor; and when the value of the first risk factor is greater than the threshold value of the first risk factor, judge that the test sample has a high risk of suffering kidney cancer;   wherein the threshold value of the first risk factor is 2.687 (fmol/μg*age).   
     
     
         3 . A method of judging a risk of suffering kidney cancer, includes the steps of:
 obtain a test sample;   detecting a PSMB9 protein in the test sample to obtain a PSMB9 protein concentration value, in which the PSMB9 protein has the amino acid sequence of SEQ ID NO: 1;   dividing the PSMB9 protein concentration value by an age to obtain a value of a second risk factor;   comparing the value of the second risk factor with a threshold value of the second risk factor; and   when the value of the second risk factor is greater than the threshold value of the second risk factor, judge that the test sample has a high risk of suffering kidney cancer;   wherein the threshold value of the second risk factor is 0.00032 [(fmol/μg)/age].   
     
     
         4 . The method of judging a risk of suffering kidney cancer in  claim 2  or  3 ; in the step of obtaining a test sample, the test sample comes from the urine of a test subject. 
     
     
         5 . The method of judging the risk of suffering kidney cancer in  claim 2  or  3 ; in the step of obtaining a test sample, further includes the following steps of:
 detecting a leukocyte of the test sample to obtain a leukocyte number value; 
 comparing the leukocyte number value with a leukocyte number threshold value; and 
 when the leukocyte number value is less than the leukocyte number threshold value, continue to detect the PSMB9 protein; 
 wherein the leukocyte number threshold value is 30/HPF, and the leukocyte number value is obtained by a general precipitation method or a conventional detection method. 
 
     
     
         6 . A method of judging a risk of suffering kidney cancer; includes the steps of:
 obtaining a post-operative test sample; the post-operative test sample is taken from a test subject that has undergone a medicine treatment or a surgical treatment or a combination of both;   detecting a PSMB9 protein in the post-operative test sample to obtain a PSMB9 protein concentration value, the PSMB9 protein has the amino acid sequence of SEQ ID NO: 1;   multiplying the PSMB9 protein concentration value by an age to obtain a value of a third risk factor;   comparing the value of the third risk factor with a threshold value of the third risk factor; and   when the value of the third risk factor is greater than the threshold value of the third risk factor, judge that the post-operative test sample has a high risk of suffering kidney cancer;   wherein the threshold value of the third risk factor is 2.687 (fmol/μg*age).   
     
     
         7 . A method of judging a risk of suffering kidney cancer; include the steps of:
 obtaining a post-operative test sample, the post-operative test sample is taken from a test subject that has undergone a medicine treatment or a surgical treatment or a combination of both;   detecting a PSMB9 protein in the post-operative test sample to obtain a PSMB9 protein concentration value; the PSMB9 protein has the amino acid sequence of SEQ ID NO: 1;   dividing the PSMB9 protein concentration value by an age to obtain a value of a fourth risk factor;   comparing the value of the fourth risk factor with a threshold value of the fourth risk factor; and   when the value of the fourth risk factor is greater than the threshold value of the fourth risk factor, judge that the post-operative test sample has a high risk of suffering kidney cancer;   wherein the threshold value of the fourth risk factor is 0.00032 [(fmol/μg)/age].   
     
     
         8 . The method of judging a risk of suffering kidney cancer in  claim 6  or  7 , in the step of obtaining a post-operative test sample, the post-operative test sample is from the urine of the test subject. 
     
     
         9 . The method of judging a risk of suffering kidney cancer in  claim 6  or  7 , in the step of obtaining a post-operative test sample; the post-operative test sample is taken from a test subject that has undergone a medicine treatment or a surgical treatment or a combination of both; the medicines are selected from the group consisting of the immunotherapeutic agent, the cytotoxic agent, the growth inhibitory agent, the radiation therapy agent, and the anti-angiogenic agent and a combination thereof. 
     
     
         10 . The method of judging a risk of suffering kidney cancer in  claim 6  or  7 , in the step of obtaining a post-operative test sample; the post-operative test sample is taken from a test subject that has undergone a medicine treatment or a surgical treatment or a combination of both, further includes the following steps of:
 detecting a leukocyte of the post-operative test sample to obtain a leukocyte number value; 
 comparing the leukocyte number value with a leukocyte number threshold value; and 
 when the leukocyte number value is less than the leukocyte number threshold value, continue to detect the PSMB9 protein; 
 wherein the leukocyte number threshold value is 30/HPF, and the leukocyte number value is obtained by a general precipitation method or a conventional detection method. 
 
     
     
         11 . The method of judging a risk of suffering kidney cancer in  claim 2  or  3  or  6  or  7 , the PSMB9 protein concentration value is determined by a liquid chromatography/multiplex reaction monitoring mass spectrometer.