Maize event dp-915635-4 and methods for detection thereof

Abstract

Embodiments disclosed herein relate to the field of plant molecular biology, specifically to DNA constructs for conferring insect resistance to a plant. Embodiments disclosed herein relate to insect resistant corn plant containing event DP-915635-4, and to assays for detecting the presence of event DP-915635-4 in samples and compositions thereof

What is claimed is: 
     
         1 . A corn plant comprising the genotype of the corn event DP-915635-4, wherein said genotype comprises a nucleotide sequence as set forth in SEQ ID NO: 26 and SEQ ID NO: 29. 
     
     
         2 . The corn plant of  claim 1 , wherein said genotype comprises the nucleotide sequence set forth in SEQ ID NO: 27 and SEQ ID NO: 30. 
     
     
         3 . The corn plant of  claim 1 , wherein said genotype comprises the nucleotide sequence set forth in SEQ ID NO: 28 and SEQ ID NO: 31. 
     
     
         4 . A DNA construct comprising an operably linked first and second expression cassette, wherein said first expression cassette comprises:
 1) an sb-RCc3 Enhancer   2) a zm-PCOa Promoter;   3) a zm-HPLV9 Intron;   4) an ipd079Ea; and   5) an sb-SCI-1B Terminator.   
     
     
         5 . A plant comprising the DNA construct of  claim 4 . 
     
     
         6 . The plant of  claim 5 , wherein said plant is a corn plant. 
     
     
         7 . A plant comprising the sequence set forth in SEQ ID NO: 21. 
     
     
         8 . A corn event DP-915635-4, wherein a representative sample of seed of said corn event has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746. 
     
     
         9 . Plant parts of the corn event of  claim 8 . 
     
     
         10 . Seed comprising corn event DP-915635-4, wherein said seed comprises a DNA molecule chosen from SEQ ID NO: 26 and SEQ ID NO: 29, wherein a representative sample of the corn event DP-915635-4 seed of has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746. 
     
     
         11 . A corn plant, or part thereof, grown from the seed of  claim 10 . 
     
     
         12 . A transgenic seed produced from the corn plant of  claim 8 . 
     
     
         13 . A transgenic corn plant, or part thereof, grown from the seed of  claim 12 . 
     
     
         14 . An isolated nucleic acid molecule comprising a nucleotide sequence chosen from SEQ ID NOs: 21, and 26-31, and full length complements thereof. 
     
     
         15 . (canceled) 
     
     
         16 . A biological sample derived from corn event DP-915635-4 plant, tissue, or seed, wherein said sample comprises a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 26 and SEQ ID NO: 29, wherein said nucleotide sequence is detectable in said sample using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said corn event DP-915635-4 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746. 
     
     
         17 . The biological sample of  claim 16 , wherein said biological sample comprises plant, plant tissue, or seed of transgenic corn event DP-915635-4 . 
     
     
         18 . The biological sample of  claim 17 , wherein said biological sample is a DNA sample extracted from the transgenic corn plant event DP-915635-4, and wherein said DNA sample comprises one or more of the nucleotide sequences chosen from SEQ ID NOs:
 21-31, and the complement thereof   
     
     
         19 . The biological sample of  claim 16 , wherein said biological sample is chosen from corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products. 
     
     
         20 . An extract derived from corn event DP-915635-4 plant, tissue, or seed and comprising a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 26 and SEQ ID NO: 29, wherein a representative sample of said corn event DP-915635-4 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746. 
     
     
         21 . The extract of  claim 20 , wherein said nucleotide sequence is detectable in said extract using a nucleic acid amplification or nucleic acid hybridization method. 
     
     
         22 . The extract of  claim 21 , wherein said extract comprises plant, plant tissue, or seed of transgenic corn plant event DP-915635-4. 
     
     
         23 . The extract of  claim 22 , wherein the extract is a composition chosen from corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products, wherein said composition comprises a detectable amount of said nucleotide sequence. 
     
     
         24 . A method of producing hybrid corn seeds comprising:
 A) sexually crossing a first inbred corn line comprising a nucleotide chosen from SEQ ID NOs: 21-31 and a second inbred line having a different genotype;   B) growing progeny from said crossing; and   C) harvesting the hybrid seed produced thereby.   
     
     
         25 . The method according to  claim 24 , wherein the first inbred corn line is a female parent. 
     
     
         26 . The method according to  claim 24 , wherein the first inbred corn line is a male parent. 
     
     
         27 . A method for producing a corn plant resistant to coleopteran pests comprising:
 A) sexually crossing a first parent corn plant with a second parent corn plant, wherein said first or second parent corn plant comprises event DP-915635-4 thereby producing a plurality of first generation progeny plants;   B) selfing the first generation progeny plant, thereby producing a plurality of second generation progeny plants; and   C) selecting from the second generation progeny plants that comprise the event DP-915635-4 and are resistant to a coleopteran pest.   
     
     
         28 . A method of producing hybrid corn seeds comprising:
 A) sexually crossing a first inbred corn line comprising the DNA construct of  claim 1  with a second inbred line not comprising the DNA construct of  claim 1 ; and   B) harvesting the hybrid seed produced thereby.   
     
     
         29 . The method of  claim 28 , further comprising the step of backcrossing a second generation progeny plant that comprises corn event DP-915635-4 to the parent plant that lacks the corn event DP-915635-4 DNA, thereby producing a backcross progeny plant that is resistant to a coleopteran pest. 
     
     
         30 . A method for producing a corn plant resistant to a corn rootworm, said method comprising:
 A) crossing a first parent corn plant with a second parent corn plant, wherein said first or second parent corn plant comprises event DP-915635-4, thereby producing a plurality of first generation progeny plants;   B) selecting a first generation progeny plant that comprises the event DP-915635-4;   C) backcrossing the first generation progeny plant of step (b) with a parent plant that lacks the corn event DP-915635-4 DNA, thereby producing a plurality of backcross progeny plants; and   D) selecting from the backcross progeny plants, a plant that comprises the event DP-915635-4;   
       wherein the selected backcross progeny plant of step (d) comprises SEQ ID NO: 21, 26, or 29. 
     
     
         31 . The method according to  claim 30 , wherein the plants of the first parent corn line are the female parents or male parents. 
     
     
         32 . Hybrid seed produced by the method of  claim 30 . 
     
     
         33 . (canceled) 
     
     
         34 . (canceled) 
     
     
         35 . (canceled) 
     
     
         36 . (canceled) 
     
     
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         38 . (canceled) 
     
     
         39 . (canceled) 
     
     
         40 . (canceled) 
     
     
         41 . (canceled) 
     
     
         42 . (canceled) 
     
     
         43 . (canceled) 
     
     
         44 . (canceled) 
     
     
         45 . (canceled) 
     
     
         46 . (canceled) 
     
     
         47 . (canceled) 
     
     
         48 . (canceled) 
     
     
         49 . (canceled) 
     
     
         50 . A corn plant comprising the genotype of the corn event DP-915635-4, wherein said genotype comprises a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 26 and SEQ ID NO: 29. 
     
     
         51 . The corn plant of  claim 50 , wherein said genotype comprises a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 27 and SEQ ID NO: 30. 
     
     
         52 . The corn plant of  claim 50 , wherein said genotype comprises a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 28 and SEQ ID NO: 31. 
     
     
         53 . The corn plant of  claim 50 , wherein the genotype comprises a nucleotide sequence having 1, 2, 3, 4, or 5 nucleotide changes in one of SEQ ID NO: 26 or SEQ ID NO: 27. 
     
     
         54 . A corn plant expressing an insecticidal protein from a fern and exhibiting a mean node-injury root rating of at least about 0.15 against corn root worm in combination with a bacterial protein exhibiting insecticidal activity against corn root worm and optionally expressing a dsRNA that targets an endogenous gene of corn rootworm, wherein the insecticidal protein, the bacterial insecticidal protein and the dsRNA display different mode of actions. 
     
     
         55 . A corn plant expressing three distinct modes of actions against western corn rootworm, wherein at least two modes are due to insecticidal proteins not derived from a bacteria.