Method

Abstract

The present invention relates generally methods and kits for detecting binding interactions, in particular protein-protein interactions, and particularly to high throughput methods for labelling, analysing, detecting and measuring protein-protein interactions.

1 - 31 . (canceled) 
     
     
         32 . A kit for carrying out the method for determining a binding interaction between a binding agent and a target, said kit comprising
 a) A binding agent library wherein each member of said binding agent library has predetermined protein binding characteristics and is associated with a known unique nucleotide sequence; and   b) a set of at least two pairs of primers for linking the nucleotide sequences associated with the binding agent; and optionally instructions for use.   
     
     
         33 . (canceled) 
     
     
         34 . The kit as claimed in  claim 32  wherein said binding agent library comprises an antibody library. 
     
     
         35 . The kit of  claim 34  wherein said antibody library comprises an antibody display library or a library of antibodies wherein each antibody is labelled with said unique nucleotide sequence. 
     
     
         36 . The kit of  claim 32  further comprising a target associated with a unique nucleotide sequence. 
     
     
         37 . The kit of  claim 36  wherein said target comprises a protein. 
     
     
         38 . The kit of  claim 32  further comprising a protein display library wherein each member of said library is associated with a unique nucleotide sequence. 
     
     
         39 . The kit of  claim 38 , wherein said protein display library is a cDNA phage display library. 
     
     
         40 . The kit of  claim 37 , wherein said protein is within a protein mixture. 
     
     
         41 . The kit of  claim 40 , wherein said protein mixture is an enriched protein mixture. 
     
     
         42 . The kit of  claim 40 , wherein said protein mixture is enriched for phosphoproteins, membrane proteins, and/or naturally or artificially modified proteins. 
     
     
         43 . The kit of  claim 32  wherein said kit does not comprise any binding agents which bind to other binding agents in the binding agent library. 
     
     
         44 . The kit of  claim 32  wherein the binding agent library is an enriched binding agent library. 
     
     
         45 . The kit of  claim 37  wherein said protein is cross-linked to other proteins within a protein mixture. 
     
     
         46 . The kit of  claim 35 , wherein the unique nucleotide sequence associated with each member of the antibody phage library is the nucleotide sequence within the phage encoding the CDR of said antibody. 
     
     
         47 . The kit of  claim 39  wherein the unique nucleotide sequence associated with each member of the cDNA phage display library is the sequence within the phage encoding the displayed protein.