Monovalent vaccine formulation and a method for preparation thereof

Abstract

The present invention discloses a vaccine formulation in accordance with an illustrative embodiment. The formulation including a live attenuated cholera vaccine strain VCUSM14P; a vaccine medium having starch, cellulose, dextrose, and yeast extract; and a phosphate buffer saline.

1 . A method for preparing a monovalent cholera vaccine against 0139, the method comprising:
 culturing of live attenuated bacterial cells of strain in a vaccine growth medium;   recovering the bacterial cells from the culture; and   suspending the recovered bacterial cells in phosphate buffer saline.   
     
     
         2 . The method of  claim 1 , wherein the wherein the vaccine medium comprising starch, cellulose, dextrose, and yeast extract. 
     
     
         3 . The method of  claim 1 , wherein the vaccine medium comprising having starch (3.25-6.75%), cellulose (2.50-4%), dextrose (18.25-24.50%), yeast extract (0.01-0.05%), and NaCl (0.05-0.15%) in a 1000 mL of phosphate buffer saline at pH 7.2+0.2