Digital polymerase chain reaction (dpcr) platforms on next generation sequencer patterned flow cell technology

Abstract

A method of detection of a target nucleic acid is provided. The method performs a digital polymerase chain reaction (dPCR) that extends the functionality of the next-generation sequencer on Patterned Flow Cell based technology. The method further includes partitioning a sample into the patterned flow cell, detecting the target nucleic acid, and counting the target nucleic acid.

1 . A method for performing digital PCR on next generation sequencer patterned flow cell comprising:
 (a). attaching the adapters to each end of the individual DNA molecules derived from biological samples;   (b) distributing the individual DNA molecules on the reaction area of the patterned flow cell;   (c) amplifying the DNA molecules on a reaction area of the patterned flow cell to form clusters;   (d) contacting the individual DNA sequences in the DNA clusters with a set of probes with fluorescent dyes for each of the different target nucleic acid sequences of interest;   (e) generating a signal in each reaction area containing the probes with fluorescent dyes; and   (f) imaging the patterned flow cell.   
     
     
         2 . The method of  claim 1 , where the step of contacting the DNA molecules bind the suitable specific target probe set with attached fluorescent dye.