Selection and use of cold-tolerant bacillus strains as biological phytostimulators

Abstract

The invention relates to a biological means for improving the yield of cultivated plants. The areas of application of the invention are agriculture, horticulture and plant protection.

1 - 11 . (canceled) 
     
     
         12 . A composition for stimulating the growth of cultivated plants, comprising a cold-tolerant  Bacillus  strain, wherein the cold-tolerant  Bacillus  strain comprises a  Bacillus atrophaeus  species or a  Bacillus simplex  species or a mixture thereof, and wherein the cold-tolerant  Bacillus simplex  species comprises ABI02S1 DSM 32020 or ABI12 DSM 32283, or a mixture thereof. 
     
     
         13 . The composition according to  claim 12 , wherein the cold-tolerant  Bacillus  strain comprises a  Bacillus atrophaeus  species, and wherein the  Bacillus atrophaeus  species comprises ABI02A1 DSM 32019, ABI03 DSM 32285, or ABI05 DSM 29418, or a mixture thereof. 
     
     
         14 . The composition according to  claim 12 , wherein the cold-tolerant  Bacillus  strain comprises a cold-tolerant  Bacillus  strain comprising  Bacillus atrophaeus  ABIO2A1 DSM 32019, ABIO3 DSM 32285, or ABIOS DSM 29418, or a mixture thereof; and a cold-tolerant  Bacillus  strain comprising  Bacillus simplex  ABIO2S1 DSM 32020 or ABI12 DSM 32283, or a mixture thereof. 
     
     
         15 . The composition according to  claim 12 , wherein the cold-tolerant  Bacillus  strain is formulated as a fluid spore suspension. 
     
     
         16 . The composition according to  claim 12 , wherein the cold-tolerant  Bacillus  strain is used for treating seeds during planting or treating seeds after planting. 
     
     
         17 . The composition according to  claim 12 , wherein the cold-tolerant  Bacillus  strain is formulated as a dry preparation (“dry stain”). 
     
     
         18 . The composition according to  claim 12 , further comprising humic acid. 
     
     
         19 . The composition according to  claim 12 , further comprising a mesophile plant growth promoting bacteria of the Bacillales group. 
     
     
         20 . The composition according to  claim 12 , further comprising a plant growth promoting fungus  Trichoderma  sp. 
     
     
         21 . The composition according to  claim 15 , wherein the fluid spore suspension comprises at least 2×10 9  spores/ml. 
     
     
         22 . The composition according to  claim 15 , wherein the fluid spore suspension comprises at least 1×10 10  spores/ml. 
     
     
         23 . The composition according to  claim 16 , wherein said treating is by pour administration or by spray administration. 
     
     
         24 . The composition according to  claim 17 , wherein the cold-tolerant  Bacillus  strain is formulated with at least 5×10 9  spores/ml. 
     
     
         25 . The composition according to  claim 17 , wherein the cold-tolerant  Bacillus  strain is formulated with at least 2×10 10  spores/ml. 
     
     
         26 . The composition according to  claim 19 , wherein said mesophile plant growth promoting bacteria of the Bacillales group comprises a  Bacillus  bacteria or a  Paenibacillus  bacteria. 
     
     
         27 . The composition according to  claim 26 , wherein said  Bacillus  bacteria comprises a  Bacillus amyloliquefaciens  ssp.  plantarum.