Compositions and methods for stabilizing coelenterazine and analogs and derivatives thereof

Abstract

Provided herein are compositions and methods for stabilizing coelenterazine and analogs or derivatives thereof, and for improving the solubility and reconstitution efficiency of coelenterazine and analogs and derivatives thereof.

1 .- 23 . (canceled) 
     
     
         24 . A composition comprising:
 a compound selected from coelenterazine and an analog or derivative thereof; and   a surface selected from a paper or fiber matrix, a plastic, a glass, or a metal.   
     
     
         25 . The composition of  claim 24 , wherein the compound is selected from coelenterazine, coelenterazine-h, coelenterazine-h-h, furimazine, JRW-0238, JRW-1743, and JRW-1744. 
     
     
         26 . The composition of  claim 24 , wherein the compound is furimazine. 
     
     
         27 . The composition of  claim 24 , wherein the composition further comprises a polymer. 
     
     
         28 . The composition of  claim 27 , wherein the polymer is a naturally-occurring biopolymer selected from pullulan, trehalose, maltose, cellulose, dextran, and a combination of any thereof. 
     
     
         29 . (canceled) 
     
     
         30 . The composition of  claim 28 , wherein the naturally-occurring biopolymer is pullulan. 
     
     
         31 . The composition of  claim 27 , wherein the polymer is a cyclic saccharide polymer or a derivative thereof, selected from α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, and hydroxypropyl β-cyclodextrin. 
     
     
         32 . The composition of  claim 31 , wherein the polymer is hydroxypropyl β-cyclodextrin. 
     
     
         33 .- 36 . (canceled) 
     
     
         37 . The composition of  claim 24 , wherein the composition further comprises a buffer, a surfactant, a reducing agent, a salt, a radical scavenger, a protein or any combination thereof. 
     
     
         38 . The composition of  claim 37 , wherein the composition further comprises a buffer selected from a phosphate buffer, tricine, and 2-(N-morpholino)ethanesulfonic acid. 
     
     
         39 . The composition of  claim 37 , wherein the composition further comprises a surfactant selected from polysorbate 20, polysorbate 40, and polysorbate 80. 
     
     
         40 . The composition of  claim 37 , wherein the composition comprises a reducing agent selected from thiourea and 6-aza-2-thiothymine. 
     
     
         41 . The composition of  claim 37 , wherein the composition further comprises a salt selected from sodium chloride and sodium phosphate. 
     
     
         42 . The composition of  claim 37 , wherein the composition further comprises a radical scavenger agent selected from ascorbic acid and sodium ascorbate. 
     
     
         43 . The composition of  claim 37 , wherein the composition further comprises a chelating agent, and the chelating agent is selected from citric acid and trans-1,2-diaminocyclohexane-tetraacetic acid. 
     
     
         44 . The composition of  claim 37 , wherein the composition further comprises a protein selected from bovine serum albumin, gelatin, and a polypeptide fraction of purified dermal collagen of porcine origin. 
     
     
         45 . The composition of  claim 24 , wherein the surface is selected from a cellulose paper, a nitrocellulose paper, a nylon paper, a cotton paper, a polyester paper, sodium carboxymethyl cellulose, a porous or polymeric membrane, a high purity cotton fiber, a cotton/rayon blended high purity cotton, and a glass microfiber. 
     
     
         46 . A method of stabilizing a compound selected from coelenterazine and an analog or derivative thereof, comprising contacting the coelenterazine compound or the analog or derivative thereof with an effective amount of a paper or fiber matrix to form a composition. 
     
     
         47 . (canceled) 
     
     
         48 . A method of improving the solubility of a compound selected from coelenterazine and an analog or derivative thereof, comprising contacting the coelenterazine compound or the analog or derivative thereof with an effective amount of a paper or fiber matrix to form a composition. 
     
     
         49 . (canceled) 
     
     
         50 . A method of improving the reconstitution rate of a compound selected from coelenterazine and an analog or derivative thereof comprising contacting the coelenterazine compound or the analog or derivative thereof with an effective amount of a paper or fiber matrix to form a composition,
 wherein the reconstitution rate for the compound is improved compared to a compound that has not been contacted with the polymer or the paper or fiber matrix.   
     
     
         51 .- 82 . (canceled) 
     
     
         83 . A kit comprising the composition of  claim 24 , wherein the composition is included in one or more containers. 
     
     
         84 .- 86 . (canceled)