Method for the Stimulation of Human Immune Cells

Abstract

Provided herein is a substance for use in a therapeutic method for stimulating cells in the human immune system, a method for producing this substance, and a method of use of the substance for stimulating cells of the human immune system in treating various diseases involving immune system disorders.

1 . A method for stimulating cells in the human immune system of a patient, comprising administering to the patient a substance prepared according to a method comprising:
 leaching homogenate of  Laminaria Japonica  and  Laminaria Angustata  algae with a mixture of an aqueous solution and an isobutanolic solution, with a 1 g:2.5 ml:2.5 ml mix ratio, to obtain a homogeneous mixture;   heating the homogenous mixture at 60° C. for one hour;   incubating the homogenous mixture for 10-15 hours at room temperature to obtain a supernatant;   withdrawing the supernatant from the homogenous mixture and centrifuging the supernatant at 3000 rpm and +4° C. for 30 minutes to obtain a centrifuged supernatant;   purging the centrifuged supernatant with argon to obtain a purged,   centrifuged supernatant and vacuum drying the purged, centrifuged supernatant for one hour at room temperature, followed by vacuum drying at 60° C. until a liquid fraction of the supernatant evaporates, to obtain a sediment;   diluting the sediment with water to produce a 5% solution and then ultracentrifuging the 5% solution at 100,000 rpm for one hour to obtain a second supernatant;   withdrawing the second supernatant and centrifuging the second supernatant in 10 kD filter tubes for one hour at 5000 rpm to obtain a lower percolated fraction;   withdrawing the lower percolated fraction and centrifuging the lower percolated fraction in 5 kD filter tubes for one hour at 5000 rpm to obtain an upper fraction detained by a filter in the filter tubes; and   withdrawing the upper fraction from the filter and diluting the upper fraction in 500 ml of NaCl saline to obtain a substance with a final concentration of 10 mg/ml.   
     
     
         2 . The method according to  claim 1 , wherein the method for preparing the substance administered to the patient further comprises, after centrifuging the supernatant and prior to purging the centrifuged supernatant, ultracentrifuging the centrifuged supernatant at 100,000 rpm for one hour. 
     
     
         3 . The method of  claim 1 , wherein the substance is administered orally to the patient for a fortnight. 
     
     
         4 . The method of  claim 3 , wherein the substance is administered in 1 ml dosage once daily for a fortnight. 
     
     
         5 . The method of  claim 3 , wherein the substance is administered before meals.