Methods for adhering tissue surfaces and materials and biomedical uses thereof

Abstract

The present invention relates to methods for adhering tissue surfaces and materials and biomedical uses thereof. In particular the present invention relates to a method for adhering a first tissue surface to a second tissue surface in a subject in need thereof, comprising the steps of adsorbing a layer of nanoparticles on at least one of the tissue surfaces, and approximating the surfaces for a time sufficient for allowing the surfaces to adhere to each other. The present invention also relates to a method for adhering a material to a biological tissue in a subject in need thereof, comprising the steps of adsorbing a layer of nanoparticles on the surface of the material and/or the biological tissue and approximating the material and the biological tissue for a time sufficient for allowing the material and the biological tissue to adhere to each other.

1 . A method for adhering a biological tissue surface to a second surface in a subject in need thereof comprising the steps of
 adsorbing a layer of nanoparticles on at least one of the biological tissue surface and the second surface and   bringing the biological tissue surface and the second surface into contact for a time sufficient for the biological tissue surface and the second surface to adhere to each other.   
     
     
         2 . The method of  claim 1 , wherein the biological tissue is selected from the group consisting of skin tissue, hair tissue, nail tissue, corneal tissue, tongue tissue, oral cavity tissue, esophageal tissue, anal tissue, urethral tissue, vaginal tissue, urinary epithelial tissue, salivary gland tissue, mammary gland tissue, lacrimal gland tissue, sweat gland tissue, prostate gland tissue, bulbourethral gland tissue, Bartholin's gland tissue, uterine tissue, respiratory and gastrointestinal tract goblet cell tissue, gastric mucosal tissue, gastric gland tissue, pancreatic tissue, spleen tissue, pulmonary tissue, pituitary gland tissue, thyroid gland tissue, parathyroid gland tissue, testicular tissue, ovarian tissue, respiratory gland tissue, gastrointestinal gland tissue, adrenal gland tissue, renal tissue, liver tissue, adipose tissue, duct cell tissue, gall bladder tissue, epidydimal tissue, vas deferens tissue, blood vessel tissue, lymph gland tissue, lymphatic duct tissue, synovial tissue, serosal tissue, squamous tissue, cochlear tissue, choroid plexus tissue, ependymal tissue, dural tissue, pia-arachnoid tissue, sclera tissue, retinal tissue, iris tissue, ciliary tissue, dental tissue, otic tissue, ligament tissue, tendon tissue, elastic cartilage tissue, fibrocartilage tissue, hyaline cartilage tissue, bone marrow tissue, intervertebral disc tissue, compact bone tissue, cancellous bone tissue, skeletal muscle tissue, cardiac muscle tissue, smooth muscle tissue, cardiac valve tissue, pericardial tissue, pleural tissue, peritoneal tissue, blood cell tissue, neuronal tissue, glial tissue, sensory transducer cell tissue, pain sensitive tissue, autonomic neuron tissue,
 peripheral nervous system tissue, cranial nerve tissue, ocular lens tissue, germ cell tissue, thymus tissue, placental tissue, fetal membrane tissue, umbilical tissue, stem cell tissue, mesodermal tissue, ectodermal tissue, endodermal tissue, autologous tissue, and allograft tissue or a combination thereof.   
     
     
         3 . The method of  claim 1 , wherein the second surface is a material to be adsorbed to the biological tissue surface. 
     
     
         4 . The method of  claim 3 , wherein the material is selected form the group consisting of membranes, scaffold materials, films, sheets, tapes, patches, meshes and medical devices. 
     
     
         5 . The method of  claim 3 , wherein the material is made of metal, glass or polymers. 
     
     
         6 . The method of  claim 3 , wherein the material is a hydrogel. 
     
     
         7 . The method of  claim 3 , wherein the material comprises a biologically active agent, a pharmaceutical agent or a radiosensitizer. 
     
     
         8 . The method of  claim 3 , wherein the material is loaded with a plurality of cells. 
     
     
         9 . The method according to  claim 1 , wherein the nanoparticles are metal oxides, alumina, silica, kaolin, hydroxyapatite, calcium carbonate, silicates such as micas quartz, zeolites or clays such as hectorite, laponite, montmorillonite, bentonite, or smectite. 
     
     
         10 . The method according to  claim 1 , wherein the nanoparticles are metal particles. 
     
     
         11 . The method according to  claim 1 , wherein the nanoparticles are made of organic polymers. 
     
     
         12 . The method according to  claim 1 , wherein the nanoparticles are made of polysaccharides. 
     
     
         13 . The method according to  claim 1 , wherein the nanoparticles are applied as an aqueous suspension of nanoparticles. 
     
     
         14 . The method according to  claim 1 , wherein the nanoparticles are applied as a suspension containing a solvent. 
     
     
         15 . The method according to  claim 1 , wherein the nanoparticles are applied as a powder. 
     
     
         16 . The method according to  claim 1 , wherein the nanoparticles are applied with a technique selected from the group consisting of coating, dipping, spraying, spreading and solvent casting. 
     
     
         17 . The method according to  claim 1 , wherein the nanoparticles are deposited on the tissue with a means selected from the group consisting of a patch, a dressing, an elastoplast and a band-aid having a plurality of capsules able to release the nanoparticles when contacted by tissue. 
     
     
         18 . The method according to  claim 3 , wherein the nanoparticles are previously adsorbed on the surface of the material. 
     
     
         19 . The method according to  claim 1 , wherein the second surface is a biological tissue and the biological tissue surface and the second surface are connected by sutures, staples, mechanical fixators, or mesh. 
     
     
         20 . The method according to  claim 1 , wherein one or both of said nanoparticles and said second surface includes a drug to control bleeding, treat infection or malignancy, or promote tissue regeneration.