System and method for organoid culture

Abstract

The present disclosure provides a system, including methods and apparatus, for culturing, monitoring, and/or analyzing organoids. In an exemplary method of organoid culture, the method may comprise disposing a scaffold in a receptacle having an open side. A sealing member may be bonded to the open side of the receptacle to create a chamber. An organoid may be formed in the chamber using the scaffold. Fluid and/or at least one substance may be introduced into the chamber from an overlying reservoir for contact with the organoid.

1 - 29 . (canceled) 
     
     
         30 . A device for culture of an organized multi-cellular structure, the device comprising:
 (a) a body defining a receptacle and at least two reservoirs, the at least two reservoirs overlying the receptacle and being in separate fluid communication with the receptacle via respective channels; and   (b) a sealing member bonded or bondable to the body at an open side of the receptacle, to create a chamber for the organized multi-cellular structure.   
     
     
         31 . The device of  claim 30 , further comprising a scaffold attached to a wall of the receptacle. 
     
     
         32 . The device of  claim 31 , wherein the scaffold is included in a hydrogel. 
     
     
         33 . The device of  claim 30 , wherein the receptacle and the at least two reservoirs are formed integrally with one another. 
     
     
         34 . The device of  claim 30 , wherein the body is injection-molded as a single piece. 
     
     
         35 . The device of  claim 30 , wherein each reservoir of the at least two reservoirs shares a wall with the receptacle. 
     
     
         36 . The device of  claim 30 , wherein each channel extends through a wall that is located intermediate the corresponding reservoir and the receptacle. 
     
     
         37 . The device of  claim 30 , further comprising a removable lid configured to cover an open top of each reservoir of the at least two reservoirs. 
     
     
         38 . The device of  claim 37 , wherein the lid comprises a flange configured to restrict lateral movement of the lid. 
     
     
         39 . The device of  claim 37 , wherein the lid comprises a fluid-tight seal with the at least two reservoirs. 
     
     
         40 . The device of  claim 30 , wherein the device is in a sterilized condition. 
     
     
         41 . The device of  claim 30 , wherein the sealing member is bonded to the body. 
     
     
         42 . The device of  claim 41 , wherein the sealing member comprises at least one of a glass or a polymer. 
     
     
         43 . The device of  claim 30 , wherein the body defines at least four reservoirs each disposed in fluid communication with the receptacle. 
     
     
         44 . The device of  claim 30 , wherein the sealing member comprises a bottom window for imaging a contents of an interior of the chamber. 
     
     
         45 . The device of  claim 30 , wherein the body comprises a pair of lateral windows to permit illumination of an interior of chamber via either of the lateral windows. 
     
     
         46 . The device of  claim 45 , wherein the pair of lateral windows are disposed opposite each other. 
     
     
         47 . The device of  claim 30 , wherein the body defines an access tube having an open top end and closed bottom end, and wherein a shared wall separates the chamber from the bottom end of the tube. 
     
     
         48 . The device of  claim 47 , wherein the access tube comprises a barrier at one end of the access tube. 
     
     
         49 . The device of  claim 30 , further comprising a linear array of substantially identical units connected to one another, wherein one of the units comprises the receptacle and the at least two reservoirs.