US2022396820A1PendingUtilityA1

Nad(p)- dependent responsive enzymes, electrodes and sensors, and methods for making and using the same

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Assignee: ABBOTT DIABETES CARE INCPriority: Mar 4, 2016Filed: Aug 10, 2022Published: Dec 15, 2022
Est. expiryMar 4, 2036(~9.6 yrs left)· nominal 20-yr term from priority
Inventors:Tianmei OuyangBenjamin J. Feldman
C12Q 1/006C12Q 1/004C12Q 1/001G01N 33/5735G01N 27/3275A61B 5/14865C12Y 106/05002C12Q 1/26C12N 11/082G01N 33/66C12Q 1/005G01N 27/27
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Claims

Abstract

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . An analyte sensor comprising an insertion tip configured to penetrate skin, the insertion tip comprising an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein, the enzyme composition comprising:
 a) nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof;   b) an NAD(P)+-dependent dehydrogenase;   c) an NAD(P)H oxidoreductase; and   d) an electron transfer agent comprising a transition metal complex;   wherein the NAD(P)+-dependent dehydrogenase and NAD(P)H oxidoreductase are present in a weight ratio ranging from 1 to 10.   
     
     
         2 . The analyte sensor of  claim 1 , wherein the weight ratio of NAD(P)+-dependent dehydrogenase to NAD(P)H oxidoreductase is from 1 to 8. 
     
     
         3 . The analyte sensor of  claim 1 , wherein the weight ratio of NAD(P)+-dependent dehydrogenase to NAD(P)H oxidoreductase is from 1 to 5. 
     
     
         4 . The analyte sensor of  claim 1 , wherein the weight ratio of NAD(P)+-dependent dehydrogenase to NAD(P)H oxidoreductase is from 1 to 2. 
     
     
         5 . The analyte sensor of  claim 1 , wherein the weight ratio of NAD(P)+-dependent dehydrogenase to NAD(P)H oxidoreductase is from 1 to 1. 
     
     
         6 . The analyte sensor of  claim 1 , wherein the NAD(P)+-dependent dehydrogenase is present in the enzyme composition in an amount from 0.01 μg to 10 μg. 
     
     
         7 . The analyte sensor of  claim 1 , wherein the NAD(P)+-dependent dehydrogenase is present in the enzyme composition in an amount from 0.05 μg to 5 μg. 
     
     
         8 . The analyte sensor of  claim 1 , wherein the NAD(P)+-dependent dehydrogenase is present in the enzyme composition in an amount from 0.5 μg to 2 μg. 
     
     
         9 . The analyte sensor of  claim 1 , wherein the amount of NAD(P)+-dependent dehydrogenase comprises from 0.01% to 10% by weight of the enzyme composition. 
     
     
         10 . The analyte sensor of  claim 1 , wherein the amount of NAD(P)+-dependent dehydrogenase comprises from 2% to 7% by weight of the enzyme composition. 
     
     
         11 . The analyte sensor of  claim 1 , wherein the NAD(P)H oxidoreductase is present in the enzyme composition in an amount from 0.01 μg to 10 μg. 
     
     
         12 . The analyte sensor of  claim 1 , wherein the NAD(P)H oxidoreductase is present in the enzyme composition in an amount from 0.05 μg to 5 μg 
     
     
         13 . The analyte sensor of  claim 1 , wherein the NAD(P)H oxidoreductase is present in the enzyme composition in an amount from 0.5 μg to 2 μg. 
     
     
         14 . The analyte sensor of  claim 1 , wherein the amount of NAD(P)H oxidoreductase comprises from 0.01% to 10% by weight of the enzyme composition. 
     
     
         15 . The analyte sensor of  claim 1 , wherein the amount of NAD(P)H oxidoreductase comprises from 2% to 7% by weight of the enzyme composition. 
     
     
         16 . The analyte sensor of  claim 1 , wherein the NAD(P)+-dependent dehydrogenase, the NAD(P)H oxidoreductase, and the electron transfer agent are present in a weight ratio of about 1:1:1. 
     
     
         17 . An analyte sensor comprising an insertion tip configured to penetrate skin, the insertion tip comprising an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein, the enzyme composition comprising:
 a. nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof;   b. an NAD(P)+-dependent dehydrogenase;   c. an NAD(P)H oxidoreductase; and   d. an electron transfer agent comprising a transition metal complex;   wherein the NAD(P)+-dependent dehydrogenase and NAD(P)H oxidoreductase are present in a weight ratio ranging from 10 to 1.   
     
     
         18 . The analyte sensor of  claim 17 , wherein the weight ratio of NAD(P)+-dependent dehydrogenase to NAD(P)H oxidoreductase ranges from 8 to 1. 
     
     
         19 . The analyte sensor of  claim 17 , wherein the weight ratio of NAD(P)+-dependent dehydrogenase to NAD(P)H oxidoreductase ranges from 8 to 1. 
     
     
         20 . The analyte sensor of  claim 17 , wherein the weight ratio of NAD(P)+-dependent dehydrogenase to NAD(P)H oxidoreductase ranges from 2 to 1. 
     
     
         21 . The analyte sensor of  claim 17 , wherein the weight ratio of NAD(P)+-dependent dehydrogenase to NAD(P)H oxidoreductase ranges from 1 to 1. 
     
     
         22 . The analyte sensor of  claim 17 , wherein the NAD(P)+-dependent dehydrogenase is present in the enzyme composition in an amount from 0.01 μg to 10 μg. 
     
     
         23 . The analyte sensor of  claim 17 , wherein the NAD(P)+-dependent dehydrogenase is present in the enzyme composition in an amount from 0.05 μg to 5 μg. 
     
     
         24 . The analyte sensor of  claim 17 , wherein the NAD(P)+-dependent dehydrogenase is present in the enzyme composition in an amount from 0.5 μg to 2 μg. 
     
     
         25 . The analyte sensor of  claim 17 , wherein the amount of NAD(P)+-dependent dehydrogenase comprises from 0.01% to 10% by weight of the enzyme composition. 
     
     
         26 . The analyte sensor of  claim 17 , wherein the amount of NAD(P)+-dependent dehydrogenase comprises from 2% to 7% by weight of the enzyme composition. 
     
     
         27 . The analyte sensor of  claim 17 , wherein the NAD(P)H oxidoreductase is present in the enzyme composition in an amount from 0.01 μg to 10 μg. 
     
     
         28 . The analyte sensor of  claim 17 , wherein the NAD(P)H oxidoreductase is present in the enzyme composition in an amount from 0.05 μg to 5 μg 
     
     
         29 . The analyte sensor of  claim 17 , wherein the NAD(P)H oxidoreductase is present in the enzyme composition in an amount from 0.5 μg to 2 μg. 
     
     
         30 . The analyte sensor of  claim 17 , wherein the amount of NAD(P)H oxidoreductase comprises from 0.01% to 10% by weight of the enzyme composition. 
     
     
         31 . The analyte sensor of  claim 17 , wherein the amount of NAD(P)H oxidoreductase comprises from 2% to 7% by weight of the enzyme composition. 
     
     
         32 . The analyte sensor of  claim 1 , wherein the NAD(P)+-dependent dehydrogenase, the NAD(P)H oxidoreductase, and the electron transfer agent are present in a weight ratio of about 1:1:1.

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