Compositions comprising hmos, their production and use for the prevention and/or treatment of viral and/or bacterial infections

Abstract

A mixture of human milk oligosaccharides that comprises or consists essentially of a) LNTri II, LNT, pLNH II and optionally lactose, or b) LNTri II, LNnT, pLNnH and optionally lactose, and is useful for preventing or treating viral or bacterial, intestinal and respiratory infections and for modulating the human gastrointestinal microbiota to increase Bifidobacterium abundance. A process for preparing the mixture and methods of crystallizing LNnT and LNT is also described.

1 . A synthetic mixture comprising,
 GlcNAcβ1-3Galβ1-4Glc (LNTri II),   a component A which is Galβ1-3GlcNAcβ1-3Galβ1-4Glc (LNT) or Galβ1-4GlcNAcβ1-3Galβ1-4Glc (LNnT),   a component B which is:
 Galβ1-3GlcNAcβ1-3 Galβ1-3GlcNAcβ1-3Galβ1-4Glc (pLNH II) when component A is LNT, or 
 Galβ1-4GlcNAcβ1-3 Galβ1-4GlcNAcβ1-3Galβ1-4Glc (pLNnH) when component A is LNnT 
   and optionally lactose.   
     
     
         2 . The mixture of  claim 1 , which contains a predominant amount of LNT or LNnT relative to the other components in the mixture. 
     
     
         3 . The mixture of  claim 1  consisting essentially of
 LNTri II, 
 a component A which is LNT or LNnT, 
 a component B which is:
 pLNH II when component A is LNT, or 
 pLNnH when component A is LNnT. 
 
 
     
     
         4 . The mixture of  claim 1  consisting essentially of
 LNTri II, 
 a component A which is LNT or LNnT, 
 a component B which is:
 pLNH II when component A is LNT, or 
 pLNnH when component A is LNnT, and 
 
 lactose. 
 
     
     
         5 . The mixture of  claim 1 , which essentially consists of LNTri II, LNnT, pLNnH and optionally lactose, and wherein the weight ratios of LNTri II, pLNnH and optionally lactose relative to LNnT are:
 LNTri II:LNnT is not more than 0.1;   pLNnH:LNnT is not more than 0.4; and   lactose:LNnT is 0 to not more than 0.8.   
     
     
         6 . The mixture of  claim 1 , which essentially consists of LNTri II, LNT, pLNH II and optionally lactose, wherein the weight ratios of LNTri II, pLNH II and optionally lactose relative to LNT are:
 LNTri II:LNT is not more than 0.2;   pLNH II:LNT is not more than 0.05; and   lactose:LNT is 0 to not more than 0.6.   
     
     
         7 . The mixture of  claim 4 , which essentially consists of LNTri II, LNnT, pLNnH and lactose, and wherein the weight ratios of lactose, LNTri II and pLNnH relative to LNnT are:
 lactose:LNnT=0.2 to 0.8, LNTri II:LNnT=0.01 to 0.05 and pLNnH:LNnT=0.1 to 0.2.   
     
     
         8 . The mixture of  claim 4 , which essentially consists of LNTri II, LNT, pLNH II and lactose, and wherein the weight ratios of lactose, LNTri II and pLNH II relative to LNT are:
 lactose:LNT=0.15 to 0.20, LNTri II:LNT=0.05 to 0.12 and pLNH II:LNT=0.005 to 0.03.   
     
     
         9 .- 17 . (canceled) 
     
     
         18 . The mixture of  claim 5 , wherein pLNnH:LNnT is not more than 0.03. 
     
     
         19 . The mixture of  claim 5 , wherein lactose:LNnT is not more than 0.2. 
     
     
         20 . A nutritional or pharmaceutical composition comprising the mixture of  claim 1 . 
     
     
         21 . A nutritional or pharmaceutical composition comprising the mixture of  claim 2 . 
     
     
         22 . A nutritional or pharmaceutical composition comprising the mixture of  claim 3 . 
     
     
         23 . A nutritional or pharmaceutical composition comprising the mixture of  claim 4 . 
     
     
         24 . A nutritional or pharmaceutical composition comprising the mixture of  claim 5 . 
     
     
         25 . A nutritional or pharmaceutical composition comprising the mixture of  claim 6 . 
     
     
         26 . A nutritional or pharmaceutical composition comprising the mixture of  claim 7 . 
     
     
         27 . A nutritional or pharmaceutical composition comprising the mixture of  claim 8 . 
     
     
         28 . A nutritional or pharmaceutical composition comprising the mixture of  claim 18 . 
     
     
         29 . A nutritional or pharmaceutical composition comprising the mixture of  claim 19 .