US2022389475A1PendingUtilityA1

Nad(p)- dependent responsive enzymes, electrodes and sensors, and methods for making and using the same

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Assignee: ABBOTT DIABETES CARE INCPriority: Mar 4, 2016Filed: Aug 10, 2022Published: Dec 8, 2022
Est. expiryMar 4, 2036(~9.6 yrs left)· nominal 20-yr term from priority
Inventors:Tianmei OuyangBenjamin J. Feldman
A61B 5/14865C12Y 106/05002G01N 27/3275G01N 33/66C12Q 1/004C12Q 1/006G01N 27/27C12Q 1/001C12N 11/082C12Q 1/26C12Q 1/005G01N 33/5735
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Claims

Abstract

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P) + ) or derivative thereof; an NAD(P) + -dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . An analyte sensor comprising an insertion tip configured to penetrate skin, the insertion tip comprising an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein, the enzyme composition comprising:
 a) nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof;   b) an NAD(P)+-dependent dehydrogenase;   c) an NAD(P)H oxidoreductase; and   d) an electron transfer agent comprising a transition metal complex;   wherein the enzyme composition is deposited on a surface of the electrode in an area of 100 mm 2  or less.   
     
     
         2 . The analyte sensor of  claim 1 , wherein the area is 75 mm 2  or less. 
     
     
         3 . The analyte sensor of  claim 1 , wherein the area is 50 mm 2  or less. 
     
     
         4 . The analyte sensor of  claim 1 , wherein the area is 25 mm 2  or less. 
     
     
         5 . The analyte sensor of  claim 1 , wherein the area is 10 mm 2  or less. 
     
     
         6 . The analyte sensor of  claim 1 , wherein the area is 5 mm 2  or less. 
     
     
         7 . The analyte sensor of  claim 1 , wherein the area is 2 mm 2  or less. 
     
     
         8 . The analyte sensor of  claim 1 , wherein the area is 1 mm 2  or less. 
     
     
         9 . The analyte sensor of  claim 1 , wherein the enzyme composition deposited on the surface of the electrode is deposited in a circular, triangular, square, rectangular, polygonal, elliptical, half circle, or crescent shape. 
     
     
         10 . The analyte sensor of  claim 1 , wherein the NAD(P)+-dependent dehydrogenase is D-3-hydroxybutyrate dehydrogenase. 
     
     
         11 . The analyte sensor of  claim 1 , wherein the NAD(P)H oxidoreductase is diaphorase. 
     
     
         12 . An analyte sensor comprising an insertion tip configured to penetrate skin, the insertion tip comprising an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an array comprising a plurality of enzyme compositions spaced apart from each other, at least one enzyme composition in the array comprising:
 a) nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof;   b) an NAD(P)+-dependent dehydrogenase;   c) an NAD(P)H oxidoreductase; and   d) an electron transfer agent comprising a transition metal complex.   
     
     
         13 . The analyte sensor of  claim 12 , wherein the plurality of enzyme compositions in the array are the same or different. 
     
     
         14 . The analyte sensor of  claim 12 , wherein the array comprises at least two enzyme compositions. 
     
     
         15 . The analyte sensor of  claim 12 , wherein the array comprises at least five enzyme compositions. 
     
     
         16 . The analyte sensor of  claim 12 , wherein the array comprises at least ten enzyme compositions. 
     
     
         17 . The analyte sensor of  claim 12 , wherein the array comprises at least twenty five enzyme compositions. 
     
     
         18 . The analyte sensor of  claim 12 , wherein the array comprises at least fifty enzyme compositions. 
     
     
         19 . The analyte sensor of  claim 12 , wherein the array comprises at least one hundred enzyme compositions. 
     
     
         20 . The analyte sensor of  claim 12 , wherein the NAD(P)+-dependent dehydrogenase is D-3-hydroxybutyrate dehydrogenase. 
     
     
         21 . The analyte sensor of  claim 12 , wherein the NAD(P)H oxidoreductase is diaphorase. 
     
     
         22 . An analyte sensor comprising an insertion tip configured to penetrate skin, the insertion tip comprising an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein, the enzyme composition comprising:
 a) nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof;   b) an NAD(P)+-dependent dehydrogenase;   c) an NAD(P)H oxidoreductase; and   d) an electron transfer agent comprising a transition metal complex; wherein at least 5% of a surface of the electrode is covered by the enzyme composition.   
     
     
         23 . The analyte sensor of  claim 22 , wherein at least 25% of the surface of the electrode is covered by the enzyme composition. 
     
     
         24 . The analyte sensor of  claim 22 , wherein at least 50% of the surface of the electrode is covered by the enzyme composition. 
     
     
         25 . The analyte sensor of  claim 22 , wherein at least 75% of the surface of the electrode is covered by the enzyme composition. 
     
     
         26 . The analyte sensor of  claim 22 , wherein at least 90% of the surface of the electrode is covered by the enzyme composition. 
     
     
         27 . The analyte sensor of  claim 22 , wherein 100% of the surface of the electrode is covered by the enzyme composition. 
     
     
         28 . The analyte sensor of  claim 22 , wherein the NAD(P)+-dependent dehydrogenase is D-3-hydroxybutyrate dehydrogenase. 
     
     
         29 . The analyte sensor of  claim 22 , wherein the NAD(P)H oxidoreductase is diaphorase.

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