Dual antigen-induced bipartite functional complementation

Abstract

The present invention relates to a set of polypeptides and its uses. In particular, the present invention relates to a set of polypeptides whereby this set comprises two polypeptides each of which comprises a targeting moiety “T” binding to an antigen “A” and a fragment of “F” of a functional domain, wherein said two polypeptides are not associated with each other in absence of a substrate that has “A” at (on) its surface and wherein, upon dimerization of “F”, the resulting dimer becomes functional. Furthermore, medical and diagnostic uses of said set are described. Moreover, the present invention relates to nucleic acid molecule(s) encoding said set of polypeptides. The present invention also relates to a vector comprising the nucleotide sequence of nucleic acid molecule(s) encoding said set of polypeptides. Furthermore, the present invention relates to pharmaceutical compositions comprising said set of polypeptides. Moreover, the present invention relates to a kit comprising said set of polypeptides.

1 . A set of polypeptides comprising:
 a first polypeptide P1 comprising
 (i) a targeting moiety T1,
 wherein said targeting moiety T1 specifically binds to an antigen A1, and 
 
 (ii) a fragment F1 of a functional domain F, 
   wherein neither said fragment F1 by itself nor said polypeptide P1 by itself is functional with respect to the function of said domain F,   and   a second polypeptide P2 comprising
 (i) a targeting moiety T2,
 wherein said targeting moiety T2 specifically binds to an antigen A2, and 
 
 (ii) a fragment F2 of said functional domain F, 
   wherein neither said fragment F2 by itself nor said polypeptide P2 by itself is functional with respect to the function of said domain F,   wherein said antigen A1 is different from said antigen A2,   wherein said polypeptide P1 and said polypeptide P2 are not associated with each other in the absence of a substrate that has both antigens A1 and A2 at its surface, and   wherein, upon dimerization of said fragment F1 of said polypeptide P1 with said fragment F2 of said polypeptide P2, the resulting dimer is functional with respect to the function of said domain F.   
     
     
         2 .- 46 . (canceled)