Reaction container and biochemical analysis method

Abstract

A reaction container includes a transparent base including a resin material and having recessed portions formed in one or more regions of the transparent base such that the recessed portions are recessed from a surface of the transparent base, and a cover member including a thermoplastic resin material and positioned on the transparent base such that the cover member forms a flow path including a gap extending over the recessed portions between the cover member and the surface of the transparent base and has a spacer portion welded by laser irradiation to the transparent base outside the one or more regions of the transparent base. The cover member absorbs infrared light and transmits light having a wavelength within a range of visible light such that the cover member has light transmittance in the range of from 480 nm to 570 nm.

What is claimed is: 
     
         1 . A reaction container, comprising:
 a transparent base comprising a resin material and having a plurality of recessed portions formed in at least one region of the transparent base such that the plurality of recessed portions is recessed from a surface of the transparent base; and   a cover member comprising a thermoplastic resin material and positioned on the transparent base such that the cover member is configured to form a flow path comprising a gap extending over the plurality of recessed portions between the cover member and the surface of the transparent base and has a spacer portion welded by laser irradiation to the transparent base outside the at least one region of the transparent base,   wherein the thermoplastic resin material of the cover member absorbs infrared light and transmits light having a wavelength within a range of visible light such that the cover member has light transmittance in a range of from 480 nm to 570 nm.   
     
     
         2 . The reaction container of  claim 1 , wherein the cover member has light transmittance of 25% or more in the range of from 480 nm to 570 nm. 
     
     
         3 . The reaction container of  claim 1 , wherein the cover member has a light transmittance gradient in a thickness direction of the cover member and is configured to absorb the infrared light such that a highest infrared absorbance is on an inner side of the cover member facing the transparent base in a direction perpendicular to the first surface of the transparent base. 
     
     
         4 . The reaction container of  claim 1 , further comprising:
 a plurality of detection electrodes positioned inside the recessed portions.   
     
     
         5 . The reaction container of  claim 1 , wherein the transparent base is formed such that the at least one region is a plurality of regions such that each of the regions has a perimeter welded to the cover member and that the regions form a plurality of reaction zones independent of each other. 
     
     
         6 . The reaction container of  claim 1 , wherein the cover member has a total light transmittance in a range of 0.01 to 60%. 
     
     
         7 . The reaction container of  claim 1 , wherein the transparent base is configured such that bright-field observation is performed on a liquid sample inside the recessed portions by the light having the wavelength within the range of visible light and that fluorescence emitted by the liquid sample is observed in response to an excitation light applied to the liquid sample inside the recessed portions through the transparent base. 
     
     
         8 . The reaction container of  claim 1 , wherein the transparent base is configured such that a signal amplification reaction is performed inside the recessed portions before observation of the fluorescence. 
     
     
         9 . The reaction container of  claim 8 , wherein the signal amplification reaction is an enzymatic reaction. 
     
     
         10 . The reaction container of  claim 9 , wherein the enzymatic reaction is an isothermal reaction. 
     
     
         11 . The reaction container of  claim 9 , wherein the enzymatic reaction is an Invader reaction. 
     
     
         12 . The reaction container of  claim 7 , wherein the liquid sample includes a detection target substance and a labeling substance specific to the detection target substance, and the detection target substance is DNA, RNA, miRNA, mRNA, or protein. 
     
     
         13 . The reaction container of  claim 12 , wherein the detection target substance is a nucleic acid, and the labeling substance is one of an enzyme, a particle, an antibody, a liposome, and a nucleic acid different from a nucleic acid of the detection target sub stance. 
     
     
         14 . The reaction container of  claim 7 , wherein the oil-based sealant liquid includes at least one of a fluorine-based oil and a silicone-based oil. 
     
     
         15 . The reaction container of  claim 1 , wherein the thermoplastic resin material of the cover member absorbs infrared light and transmits light having a wavelength within a range of visible light such that the cover member has light transmittance in a range of from 480 nm to 632 nm. 
     
     
         16 . The reaction container of  claim 15 , wherein the cover member has light transmittance of 25% or more in the range of from 480 nm to 632 nm. 
     
     
         17 . The reaction container of  claim 15 , wherein the cover member has a light transmittance gradient in a thickness direction of the cover member and is configured to absorb the infrared light such that a highest infrared absorbance is on an inner side of the cover member facing the transparent base in a direction perpendicular to the first surface of the transparent base. 
     
     
         18 . The reaction container of  claim 15 , further comprising:
 a plurality of detection electrodes positioned inside the recessed portions.   
     
     
         19 . The reaction container of  claim 15 , wherein the transparent base is formed such that the at least one region is a plurality of regions such that each of the regions has a perimeter welded to the cover member and that the regions form a plurality of reaction zones independent of each other. 
     
     
         20 . The reaction container of  claim 15 , wherein the cover member has a total light transmittance in a range of 0.01 to 60%.