ANTI-Eva1 PROTEIN ANTIBODY

Abstract

In order to provide an antibody having high therapeutic and prophylactic effects against cancer and the like, three types of mouse monoclonal antibodies were prepared which exhibit high affinities for a human-derived Eva1 protein. Moreover, constant regions of these antibodies were substituted with human-derived constant regions to also prepare chimeric antibodies. Further, these mouse antibodies and chimeric antibodies were found to have high ADCC and/or CDC activities. Furthermore, it was also revealed that administering these antibodies to mice having been subjected to melanoma cell administration suppresses the metastasis and the like of the cells to the lungs.

1 . An anti-Eva1 antibody molecule comprising a light chain variable region comprising the same complementarity-determining regions (CDRs) 1-3 as the light chain variable region set forth in SEQ ID NO: 3; and further comprising a heavy chain variable region comprising the same CDRs 1-3 as the heavy chain variable region set forth in SEQ ID NO: 9. 
     
     
         2 . The antibody molecule according to  claim 1 , wherein said antibody molecule comprises a human constant region. 
     
     
         3 . The antibody molecule according to  claim 1 , wherein said antibody molecule is a humanized antibody. 
     
     
         4 . The antibody molecule according to  claim 1 , wherein said antibody molecule has at least one cytotoxicity activity selected from the group consisting of antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). 
     
     
         5 . A pharmaceutical composition comprising the antibody molecule according to  claim 1  as an active ingredient, and further comprising a pharmaceutically acceptable carrier or a pharmaceutically acceptable medium.