Peptides and combination thereof for use in the immunotherapy against cancers

Abstract

The present invention relates to peptides, proteins, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated T-cell peptide epitopes, alone or in combination with other tumor-associated peptides that can for example serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses, or to stimulate T cells ex vivo and transfer into patients. Peptides bound to molecules of the major histocompatibility complex (MHC), or peptides as such, can also be targets of antibodies, soluble T-cell receptors, and other binding molecules.

What is claimed: 
     
         1 . A method of treating a patient who has cancer that presents a peptide consisting of the amino acid sequence of AEGGKVPIKW (SEQ ID NO: 218) in a complex with an MHC class I molecule on the surface of cancer cells, comprising administering to said patient a composition comprising a population of activated CD8+ cytotoxic T cells that bind a peptide consisting of the amino acid sequence of AEGGKVPIKW (SEQ ID NO: 218 in a complex with an MHC class I molecule,
 wherein said cancer is selected from the group consisting of brain cancer, renal cell carcinoma, head and neck squamous cell carcinoma, non-small cell lung cancer adenocarcinoma, prostate cancer and benign prostate hyperplasia, and uterine cancer.   
     
     
         2 . The method of  claim 1 , wherein the composition further comprises an adjuvant selected from imiquimod, GM-CSF, poly-(ICLC), interleukin (IL)-1, IL-2, IL-4, IL-7, IL-12, IL-13, IL-15, IL-21, and IL-23. 
     
     
         3 . The method of  claim 1 , wherein the activated CD8+ cytotoxic T cells are produced by contacting CD8+ cytotoxic T cells with an antigen presenting cell that expresses the peptide in a complex with an MHC class I molecule on the surface of the antigen presenting cell, for a period of time sufficient to activate said CD8+ cytotoxic T cells. 
     
     
         4 . The method of  claim 1 , wherein the cancer is brain cancer. 
     
     
         5 . The method of  claim 1 , wherein the cancer is renal cell carcinoma. 
     
     
         6 . The method of  claim 1 , wherein the cancer is head and neck squamous cell carcinoma. 
     
     
         7 . The method of  claim 1 , wherein the cancer is non-small cell lung cancer adenocarcinoma. 
     
     
         8 . The method of  claim 1 , wherein the cancer is prostate cancer and benign prostate hyperplasia. 
     
     
         9 . The method of  claim 1 , wherein the cancer is uterine cancer. 
     
     
         10 . The method of  claim 2 , wherein the adjuvant is IL-15. 
     
     
         11 . A method of eliciting an immune response in a patient who has cancer that presents a peptide consisting of the amino acid sequence of AEGGKVPIKW (SEQ ID NO: 218) in a complex with an MHC class I molecule on the surface of cancer cells, comprising administering to said patient a composition comprising a population of activated CD8+ cytotoxic T cells that bind a peptide consisting of the amino acid sequence of AEGGKVPIKW (SEQ ID NO: 218) in a complex with an MHC class I molecule, wherein said cancer is selected from the group consisting of brain cancer, renal cell carcinoma, head and neck squamous cell carcinoma, non-small cell lung cancer adenocarcinoma, prostate cancer and benign prostate hyperplasia, and uterine cancer. 
     
     
         12 . The method of  claim 11 , wherein the composition further comprises an adjuvant selected from imiquimod, GM-CSF, poly-(ICLC), interleukin (IL)-1, IL-2, IL-4, IL-7, IL-12, IL-13, IL-15, IL-21, and IL-23. 
     
     
         13 . The method of  claim 11 , wherein the activated CD8+ cytotoxic T cells are produced by contacting CD8+ cytotoxic T cells with an antigen presenting cell that expresses the peptide in a complex with an MHC class I molecule on the surface of the antigen presenting cell, for a period of time sufficient to activate said CD8+ cytotoxic T cells. 
     
     
         14 . The method of  claim 11 , wherein the cancer is brain cancer. 
     
     
         15 . The method of  claim 11 , wherein the cancer is renal cell carcinoma. 
     
     
         16 . The method of  claim 11 , wherein the cancer is head and neck squamous cell carcinoma. 
     
     
         17 . The method of  claim 11 , wherein the cancer is non-small cell lung cancer adenocarcinoma. 
     
     
         18 . The method of  claim 11 , wherein the cancer is prostate cancer and benign prostate hyperplasia. 
     
     
         19 . The method of  claim 11 , wherein the cancer is uterine cancer. 
     
     
         20 . The method of  claim 12 , wherein the adjuvant is IL-15.