Methods of treatment using titanium and silver nanoparticles made with an intracellular extract of fomes fomentarious

Abstract

Silver and titanium oxide nanoparticles produced with Fomes fomentarius or Fomitopsis pinicola aqueous extracts and methods for treating bacterial infections or cancer using them.

1 .  Fomes fomentarius -TiO 2  nanoparticles that comprise a rutile phase  or Fomes fomentarius -Ag nanoparticles comprising a fcc structure;
 wherein said  Fomes fomentarius -TiO 2  nanoparticles exhibit by Fourier transform infrared (FT-IR) spectroscopy absorption peaks between 766-1630 cm −1  corresponding to Ti—O, aliphatic C—N, and aromatic C═N bands; and   wherein said  Fomes fomentarius -Ag nanoparticles exhibit by Fourier transform infrared (FT-IR) spectroscopy a broad adsorption peak at about 3290 cm −1  corresponding to hydroxyl (—OH) and N—H stretching of primary amines.   
     
     
         2 . A composition comprising a pharmaceutically acceptable carrier or excipient and at least one nanoparticle of  claim 1 . 
     
     
         3 . A composition comprising a soap, surfactant, bleach, hydrogen peroxide, or other cleaner and at least one nanoparticle of  claim 1 . 
     
     
         4 . A method for making  Fomes fomentarius -Ag nanoparticles or  Fomes fomentarius -TiO 2  nanoparticles of  claim 1  comprising:
 combining an aqueous extract of  Fomes fomentarius  with silver nitrate under conditions suitable for producing  Fomes fomentarius -Ag nanoparticles; or 
 combining an aqueous extract of  Fomes fomentarius  with titanium isopropoxide under conditions suitable for producing  Fomes fomentarius -TiO 2  nanoparticles. 
 
     
     
         5 . A method for treating a microbial infection or cancer, comprising:
 administering to a subject in need thereof at least one of  Fomes fomentarius -TiO 2  nanoparticles or  Fomes fomentarius -Ag nanoparticles of  claim 1  in an amount sufficient to inhibit growth or viability of a microorganism or proliferation or viability of the cancer.   
     
     
         6 . The method of  claim 5 , wherein  Fomes fomentarius -Ag nanoparticles having an average diameter ranging from 10 to 30 nm are administered. 
     
     
         7 . The method of  claim 5 , wherein  Fomes fomentarius -TiO 2  nanoparticles having an average diameter ranging from 80 to 120 nm are administered. 
     
     
         8 . The method of  claim 5 , wherein the  Fomes fomentarius -TiO 2  nanoparticles are made using an aqueous extract of  Fomes fomentarius  and titanium isopropoxide and the  Fomes fomentarius -Ag nanoparticles are made using an aqueous extract of  Fomes fomentarius  and silver nitrate. 
     
     
         9 . The method of  claim 5 , wherein said subject is in need of treatment of a bacterial infection. 
     
     
         10 . The method of  claim 5 , wherein said subject is in need of treatment for cancer 
     
     
         11 .  Fomitopsis pinicola -TiO 2  nanoparticles that comprise a rutile phase or  Fomitopsis pinicola -Ag nanoparticles comprising a fcc structure;
 wherein said  Fomitopsis pinicola -TiO 2  nanoparticles exhibit by Fourier transform infrared (FT-IR) spectroscopy adsorption at 1000, 1414, and 1644 cm −1  respectively corresponding to C═N, C—N and (TiO 2 , OH and C═O); and   wherein said  Fomitopsis pinicola -Ag nanoparticles exhibit by Fourier transform infrared (FT-IR) spectroscopy peak absorption of linear aliphatic amines (C—N) at 1000-1040 cm −1 .   
     
     
         12 . A composition comprising a pharmaceutically acceptable carrier or excipient and at least one nanoparticle of  claim 11 . 
     
     
         13 . A composition comprising a soap, surfactant, bleach, hydrogen peroxide, or other cleaner and at least one nanoparticle of  claim 11 . 
     
     
         14 . A method for making  Fomitopsis pinicola -Ag nanoparticles or  Fomitopsis pinicola -TiO 2  nanoparticles of  claim 11  comprising:
 combining an aqueous extract of  Fomitopsis pinicola  with silver nitrate under conditions suitable for producing  Fomitopsis pinicola -Ag nanoparticles; or 
 combining an aqueous extract of  Fomitopsis pinicola  with titanium isopropoxide under conditions suitable for producing  Fomitopsis pinicola -TiO 2  nanoparticles. 
 
     
     
         15 . A method for treating a microbial infection or cancer, comprising:
 administering to a subject in need thereof at least one of  Fomitopsis pinicola -TiO 2  nanoparticles or  Fomitopsis pinicola -Ag nanoparticles of  claim 11  in an amount sufficient to inhibit growth or viability of a microorganism or proliferation or viability of the cancer.   
     
     
         16 . The method of  claim 15 , wherein  Fomitopsis pinicola -Ag nanoparticles having an average diameter ranging from 10 to 30 nm are administered. 
     
     
         17 . The method of  claim 15 , wherein  Fomitopsis pinicola -TiO 2  nanoparticles having an average diameter ranging from 80 to 120 nm are administered 
     
     
         18 . The method of  claim 15 , wherein the  Fomitopsis pinicola -TiO 2  nanoparticles are made using an aqueous extract of  Fomitopsis pinicola  and titanium isopropoxide and the  Fomitopsis pinicola -Ag nanoparticles are made using an aqueous extract  of Fomitopsis pinicola  and silver nitrate. 
     
     
         19 . The method of  claim 15 , wherein said subject is in need of treatment of a bacterial infection. 
     
     
         20 . The method of  claim 15 , wherein said subject is in need of treatment for cancer.