Xanthomonas resistant brassica oleracea plants

Abstract

The present disclosure provides Brassica oleracea plants exhibiting broad spectrum resistance to Xanthomonas campestris pv. campestris. Such plants may comprise novel introgressed genomic regions associated with disease resistance from Brassica oleracea var. capitata. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.

What is claimed is: 
     
         1 . A cultivated variety of a  Brassica oleracea  plant comprising an introgression from  Brassica oleracea  var. capitata chromosome 3 that confers broad-spectrum resistance to  Xanthomonas campestris  pv. campestris relative to a plant lacking said introgression, and wherein said resistance is co-dominant and additive. 
     
     
         2 . A broccoli or cauliflower plant comprising an introgression from  Brassica oleracea  var. capitata chromosome 3 that confers broad-spectrum resistance to  Xanthomonas campestris  pv. campestris relative to a plant lacking said introgression, and wherein said resistance is co-dominant and additive. 
     
     
         3 . The plant of  claim 1 , wherein said introgression lacks an allele genetically linked thereto conferring retarded plant growth. 
     
     
         4 . The plant of  claim 1 , wherein said introgression comprises a chromosomal segment from  Brassica oleracea  var. capitata flanked by marker NH0265157 (SEQ ID NO:5) and marker NH0266951 (SEQ ID NO:12) in said plant. 
     
     
         5 . The plant of  claim 1 , wherein said introgression comprises a chromosomal segment from  Brassica oleracea  var. capitata at marker NH0265597 (SEQ ID NO:11) in said plant. 
     
     
         6 . The plant of  claim 1 , wherein said introgression is about 2 cM or less in size. 
     
     
         7 . The plant of  claim 1 , wherein said broad-spectrum resistance comprises resistance to a plurality of  Xanthomonas campestris  pv. campestris pathovars. 
     
     
         8 . The plant of  claim 7 , wherein said broad-spectrum resistance comprises resistance to  Xanthomonas campestris  pv. campestris pathovars 1 and 4. 
     
     
         9 . The plant of  claim 7 , wherein said broad-spectrum resistance comprises resistance to  Xanthomonas campestris  pv. campestris pathovars 1, 4, 6 and 9. 
     
     
         10 . The plant of  claim 1 , wherein said plant is a broccoli, cauliflower, sprouting broccoli, Brussels sprouts, white cabbage, red cabbage, savoy cabbage, curly kale cabbage, turnip cabbage or Portuguese cabbage plant. 
     
     
         11 . The plant of  claim 1 , wherein said plant is not a white cabbage plant. 
     
     
         12 . The plant of  claim 1 , wherein the plant is homozygous for said introgression. 
     
     
         13 . The plant of  claim 1 , wherein a sample of seed comprising said introgression was deposited under ATCC Accession Number PTA-123409. 
     
     
         14 . A seed that produces the plant of  claim 1 . 
     
     
         15 . A plant part of the plant of  claim 1 . 
     
     
         16 . The plant part of  claim 15 , wherein the plant part is a cell, a seed, a root, a stem, a leaf, a head, a flower, or pollen. 
     
     
         17 . An introgression fragment comprising a chromosomal segment from  Brassica oleracea  var. capitata flanked by marker NH0265157 (SEQ ID NO:5) and marker NH0266951 (SEQ ID NO:12). 
     
     
         18 . An introgression fragment comprising a chromosomal segment from  Brassica oleracea  var. capitata at marker NH0265597 (SEQ ID NO:11). 
     
     
         19 . An introgression fragment comprising a chromosomal segment from  Brassica oleracea  var. capitata wherein said fragment confers broad-spectrum resistance to  Xanthomonas campestris  pv. campestris and lacks an allele genetically linked thereto conferring retarded plant growth. 
     
     
         20 . An introgression fragment comprising a chromosomal segment from  Brassica oleracea  var. capitata wherein said fragment confers broad-spectrum resistance to  Xanthomonas campestris  pv. campestris and lacks an allele genetically linked thereto conferring retarded plant growth, wherein a sample of seed comprising said introgression was deposited under ATCC Accession Number PTA-123409. 
     
     
         21 . A method for producing a cultivated variety of a  Brassica oleracea  plant with improved resistance to  Xanthomonas campestris  pv. campestris, comprising introgressing into said plant variety a chromosomal segment from  Brassica oleracea  var. capitata chromosome 3 that confers broad-spectrum resistance to  Xanthomonas campestris  pv. campestris relative to a plant lacking said introgression, and wherein said resistance is co-dominant and additive. 
     
     
         22 . The method of  claim 21 , wherein said introgressing comprises:
 a) crossing a plant comprising said chromosomal segment with itself or with a second  Brassica oleracea  plant of a different genotype to produce one or more progeny plants; and   b) selecting a progeny plant comprising said chromosomal segment.   
     
     
         23 . The method of  claim 22 , wherein selecting a progeny plant comprises detecting at least one allele flanked by marker NH0265157 (SEQ ID NO:5) and marker NH0266951 (SEQ ID NO:12). 
     
     
         24 . The method of  claim 22 , wherein selecting a progeny plant comprises detecting at least one allele at marker NH0265597 (SEQ ID NO:11). 
     
     
         25 . The method of  claim 21 , wherein said plant variety is a broccoli, cauliflower, sprouting broccoli, Brussels sprouts, white cabbage, red cabbage, savoy cabbage, curly kale cabbage, turnip cabbage or Portuguese cabbage plant variety. 
     
     
         26 . The method of  claim 21 , wherein said resistance comprises resistance to a plurality of  Xanthomonas campestris  pv. campestris pathovars. 
     
     
         27 . The method of  claim 26 , wherein said resistance comprises resistance to  Xanthomonas    campestris  pv. campestris pathovars 1 and 4. 
     
     
         28 . The method of  claim 26 , wherein said resistance comprises resistance to  Xanthomonas    campestris  pv. campestris pathovars 1, 4, 6 and 9. 
     
     
         29 . The method of  claim 22 , wherein the progeny plant is an F2-F6 progeny plant. 
     
     
         30 . The method of  claim 22 , wherein said crossing comprises backcrossing. 
     
     
         31 . The method of  claim 30 , wherein said backcrossing comprises from 2-7 generations of backcrosses. 
     
     
         32 . The method of  claim 21 , wherein a sample of seed comprising said chromosomal segment was deposited under ATCC Accession Number PTA-123409. 
     
     
         33 . A  Brassica oleracea  plant produced by the method of  claim 20 . 
     
     
         34 . A method of producing food or feed comprising obtaining a plant according to  claim 1  or  33 , or a part thereof, and producing said food or feed from said plant or part thereof.