S-layer protein 2d lattice coupled detergent-free gpcr bioelectronic interfaces, devices, and methods for use thereof

Abstract

The invention includes a bioelectronic interface comprising a self-assembling unit, wherein the self-assembling unit comprises a variant GPCR fusion protein bound to an S-layer fusion protein. The invention also encompasses a biosensor or device comprising the bioelectronic interface and methods of screening for a ligand of a GPCR.

1 - 48 . (canceled) 
     
     
         49 . A GPCR variant fusion protein comprising a variant GPCR fused to an Fc region, wherein the variant GPCR is a water-soluble variant of a native GPCR wherein a plurality of amino acid residues Leucine (L), isoleucine (I), valine (V), and phenylalanine (F) within the seven-transmembrane α-helical domain of the native GPCR are replaced with glutamine (Q), threonine (T), threonine (T), and tyrosine (Y), respectively; and wherein the binding moiety is fused to the C-terminus of the variant GPCR. 
     
     
         50 . (canceled) 
     
     
         51 . The fusion protein of  claim 49 , wherein the Fc regions is a human IgG1 Fc region. 
     
     
         52 . (canceled)