A quality detection method of an immunochip

Abstract

A quality detection method of an immunochip is disclosed. At least part of a surface of the immunochip is a modified surface with modifying groups. The method comprises: applying a detection solution containing a labeled antibody on the modified surface of the immunochip, the labeled antibody is an antibody connected with a labeling substance, and the labeled antibody is capable of reacting with the modifying groups and bonding to the modifying groups; removing the labeled antibody that has not reacted with the modifying groups, and detecting the content of residual labeled antibody on the modified surface; and identifying the quality of the immunochip according to the content of residual labeled antibody.

1 . A quality detection method of an immunochip, at least part of a surface of the immunochip being a modified surface with modifying groups, wherein the method comprises the following steps:
 applying a detection solution containing a labeled antibody on the modified surface of the immunochip, wherein the labeled antibody is an antibody connected with a labeling substance, and the labeled antibody is capable of reacting with the modifying groups and bonding to the modifying groups;   removing the labeled antibody that has not reacted with the modifying groups, and detecting the content of residual labeled antibody on the modified surface; and   identifying the quality of the immunochip according to the content of residual labeled antibody.   
     
     
         2 . The method according to  claim 1 , wherein, the modifying groups are carboxyl groups. 
     
     
         3 . The method according to  claim 2 , wherein, before applying the detection solution containing the labeled antibody on the modified surface of the immunochip, the method further comprises:
 performing an activation treatment on the immunochip for increasing the activity of the carboxyl groups modified on the modified surface of the immunochip.   
     
     
         4 . The method according to  claim 3 , wherein, the activation treatment on the immunochip comprises:
 placing the immunochip in an activation solution and keeping for a first predetermined time, the activation solution is a mixed solution of a 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution and a N-hydroxysuccinimide solution.   
     
     
         5 . The method according to  claim 4 , wherein, the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution and the N-hydroxysuccinimide solution are mixed in a ratio of 1:0.8 to 1:1.2. 
     
     
         6 . The method according to  claim 4 , wherein, the first predetermined time is 10 minutes to 20 minutes. 
     
     
         7 . The method according to  claim 1 , wherein, between applying the detection solution containing the labeled antibody on the modified surface of the immunochip and removing the labeled antibody that has not reacted with the modifying groups, the method further comprises:
 placing the immunochip in a predetermined environment and keeping for a second predetermined time.   
     
     
         8 . The method according to  claim 7 , wherein, the second predetermined time is greater than or equal to 8 hours. 
     
     
         9 . The method according to  claim 7 , wherein, the predetermined environment evades the light, and the predetermined environment has an environmental relative humidity of greater than 50%, and an environmental temperature of 2° C. to 6° C. 
     
     
         10 . The method according to  claim 1 , wherein:
 the labeling substance is a fluorescent substance, and the labeled antibody is a fluorescent antibody; and   identifying the quality of the immunochip according to the content of residual labeled antibody comprises: identifying the quality of the immunochip according to the fluorescence intensity of the fluorescent substance of the residual fluorescent antibody.   
     
     
         11 . The method according to  claim 10 , wherein: identifying the quality of the immunochip according to the fluorescence intensity of the fluorescent substance of the residual fluorescent antibody fluorescent antibody comprises:
 acquiring a fluorescence image of the immunochip;   randomly selecting a plurality of fluorescent regions of a predetermined area from the fluorescent image, and acquiring the fluorescence intensity value of each fluorescent region; and   acquiring an average value of the fluorescence intensity values of the plurality of fluorescent regions, and identifying the quality of the immunochip according to the average value.   
     
     
         12 . The method according to  claim 1 , wherein,
 the modified surface includes a quality control area and an experimental area;   applying the detection solution containing the labeled antibody on the modified surface of the immunochip comprises: applying the detection solution containing the labeled antibody on the quality control area.   
     
     
         13 . The method according to  claim 1 , wherein, before applying the detection solution containing the labeled antibody on the modified surface of the immunochip, the method further comprises:
 performing an activation treatment on the immunochip for increasing the activity of the carboxyl groups modified on the modified surface of the immunochip.   
     
     
         14 . The method according to  claim 4 , wherein, the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution has a concentration of 6 wt % to 8 wt %; and the N-hydroxysuccinimide solution has a concentration of 1 wt % to 3 wt %. 
     
     
         15 . The method according to  claim 12 , wherein, the quality control area and the experimental area have the same content of modifying groups. 
     
     
         16 . The method according to  claim 12 , wherein, detecting the content of residual labeled antibody on the modified surface comprising fluorescence microscopy, laser confocal microscopy, ultraviolet-visible spectroscopy, gas chromatography, or liquid chromatography.