Culture medium composition and method of culturing cell or tissue using thereof

Abstract

The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, thus affording an effect of preventing sedimentation thereof, and the like

1 . A medium composition comprising (i) deacylated gellan gum or a salt thereof, and (ii) a polysaccharide other than deacylated gellan gum or a salt thereof, wherein the polysaccharide other than deacylated gellan gum or a salt thereof is at least one kind selected from the group consisting of xanthan gum, alginic acid, carageenan, diutan gum, and a salt thereof. 
     
     
         2 . The medium composition according to  claim 1 , wherein the polysaccharide other than deacylated gellan gum or a salt thereof is alginic acid or a salt thereof. 
     
     
         3 . The medium composition according to  claim 1 , wherein the deacylated gellan gum or salt thereof and the polysaccharide other than deacylated gellan gum or a salt thereof are present at a concentration which allows cells or a tissue to be cultured in suspension standing culture. 
     
     
         4 . The medium composition according to  claim 1 , wherein the composition has a viscosity of not more than 8 mPa·s at 37° C. 
     
     
         5 . The medium composition according to  claim 1 , wherein the polysaccharide other than deacylated gellan gum or a salt thereof is at least one kind selected from the group consisting of methylcellulose, locust bean gum, and a salt thereof. 
     
     
         6 . The medium composition according to  claim 5 , wherein the polysaccharide other than deacylated gellan gum or a salt thereof is methylcellulose or a salt thereof. 
     
     
         7 . The medium composition according to  claim 5 , wherein the deacylated gellan gum or salt thereof and the polysaccharide other than deacylated gellan gum or a salt thereof are present at a concentration which allows cells or a tissue to be cultured in suspension standing culture. 
     
     
         8 . A cell or tissue culture comprising the medium composition according to  claim 1  and cells or tissues. 
     
     
         9 . A method of culturing a cell or tissue, comprising cultivating the cell or tissue in the medium composition according to  claim 1 .