Novel eukaryotic cells and methods for recombinantly expressing a product of interest

Abstract

The disclosure pertains to novel eukaryotic cell suitable for recombinant production of a product of interest, wherein the effect of the expression product of an endogenous gene C12orf35 is impaired in said cell, thereby increasing their productivity when recombinantly expressing a polypeptide of interest. Furthermore, the present disclosure provides associated technologies wherein such host cells are used in recombinant production technologies.

1 - 29 . (canceled) 
     
     
         30 . A method for analyzing eukaryotic cells for their suitability as host cells for recombinant expression of a product of interest, comprising analyzing directly or indirectly whether the effect of the expression product of endogenous gene C12orf35 is impaired in said cells. 
     
     
         31 . The method according to  claim 30 , wherein directly analyzing comprises analyzing whether the functional expression of gene C12orf35 is reduced or eliminated in said cells. 
     
     
         32 . The method according to  claim 30 , wherein prior to analysis, the eukaryotic cells are treated with an agent that induces chromosome breakage and wherein the analysis comprises analyzing whether treatment with said agent resulted in a deletion of a portion of a chromosome which includes gene C12orf35. 
     
     
         33 . The method according to  claim 30 , wherein the eukaryotic cells are hamster cells and the method comprises analyzing whether expression of one or more genes located in the telomeric region of chromosome 8 and being selected from the group consisting of the Tmtc1 gene and genes located telomeric of the Tmtc1 gene is eliminated or reduced, thereby analyzing whether the expression of gene C12orf35 is reduced or eliminated in said cells. 
     
     
         34 . The method according to  claim 30 , comprising additionally analyzing directly or indirectly whether the effect of FAM60A is impaired in said cells. 
     
     
         35 . The method according to  claim 30 , wherein prior to analysis, the eukaryotic cells are transfected with a heterologous polynucleotide encoding a product of interest and a heterologous polynucleotide encoding a selectable marker, and wherein prior to analysis at least one selection step is performed to identify successfully transfected host cells. 
     
     
         36 . The method according to  claim 30 , wherein a plurality of cell clones is analyzed for discriminating between stable and unstable and/or between high and low producing cell clones and wherein one or more cell clones are selected as production clone wherein the expression of gene C12orf35 and optionally FAM60A is reduced or eliminated.