Functionalized support material and methods of making and using functionalized support material

Abstract

Methods of making functionalized support material am disclosed. Functionalized support material suitable for use in chromatography columns or cartridges, such as in a high pressure liquid chromatography (HPLC) column or a fast protein liquid chromatography (FPLC) column, is also disclosed. Chromatography columns or cartridges containing the functionalized support material, and methods of using functionalized support material, such as a media (e.g., chromatographic material) in a chromatography column or cartridge, are also disclosed.

1 - 55 . (canceled) 
     
     
         56 . A chromatographic material comprising:
 silica support material and polymer chains extending from a surface of the silica support material, the polymer chains being covalently bonded to the surface through a divalent sulfur bond —S—;   wherein:
 the polymer chains comprise the reaction product of a thiol-functionalized silica support and monomers comprising:
 2-acrylamido-2-methyl-1-propanesulfonic acid, vinylsulfonic acid, or a combination thereof; or 
 2-acrylamido-2-methyl-1-propanesulfonic acid, methacrylic acid, acrylic acid, N,N-diethylaminoethyl acrylate, (3-acrylamidopropyl)-trimethylammonium chloride, diallyldimethylammonium chloride, or a combination of two or more thereof, and 
 
 the thio-functionalized silica support is the reaction product of silica particles and (A) an epoxy silane and 1,2-ethanedithiol, or (B) 3-mercaptopropyl-trimethoxysilane. 
   
     
     
         57 . The chromatographic material of  claim 56 , wherein the support material further comprises at least one bifunctional second compound extending from the surface of the support material, each of the at least one bifunctional compound comprising (i) one or more functional groups capable of bonding the bifunctional compound to the surface, and (ii) one or more hydrophilic groups, and/or wherein the functionalized support material comprises a covalent bond linkage between the support material and each of the polymer chains, wherein the covalent bond linkage consists of one or more atoms selected from the group consisting of C, O, Si and S, and/or wherein the support material comprises particulate support material. 
     
     
         58 . The chromatographic material of  claim 56 , wherein the silica support material comprises particulates, fibers plates, membranes, monoliths, or a combination of any two or more thereof. 
     
     
         59 . The chromatographic material of  claim 56 , wherein the silica support material comprises particulate materials.