Mrna functionalization method

Abstract

The present invention provides a functionalized mRNA including mRNA and double-stranded RNA including at least one RNA oligomer hybridized with mRNA. Functionalized mRNA is provided according to this configuration.

1 . A method for stabilizing a carrier, which comprises:
 allowing a carrier to load therein an mRNA encoding a target gene and at least one RNA oligomer hybridizing with the mRNA, wherein   the RNA oligomer comprises:   (a) an RNA sequence consisting of a sequence of 12 to 40 nucleotides complementary to the sequence of the mRNA, or   (b) an RNA sequence having an identity of 90% or more to a sequence of 12 to 40 nucleotides complementary to the sequence of the mRNA, and hybridizing with the mRNA,   and the RNA oligomer is chemically unmodified or is chemically modified.   
     
     
         2 . The method of  claim 1 , wherein the RNA sequence consists of a sequence of 15 to 23 nucleotides. 
     
     
         3 . The method of  claim 1 , wherein the RNA sequence consists of a sequence of 17 nucleotides. 
     
     
         4 . The method of  claim 1 , wherein the RNA oligomer comprises said RNA sequence (a) or (b) that is chemically modified. 
     
     
         5 . The method of  claim 4 , wherein the chemical modification is performed on the 5′-terminus or 3′-terminus of the sequence of the RNA oligomer via an overhang sequence consisting of 1 to 5 nucleotides. 
     
     
         6 . The method of  claim 5 , wherein the overhang sequence is a sequence consisting of 2 nucleotides. 
     
     
         7 . The method of  claim 4 , wherein the chemical modification is modification with a hydrophobic group. 
     
     
         8 . The method of  claim 4 , wherein the modification with a hydrophobic group is cholesterol modification. 
     
     
         9 . The method of  claim 4 , wherein the chemical modification is polyethylene glycol modification. 
     
     
         10 . The method of  claim 1 , wherein the carrier is a polymeric micelle or a lipidic mRNA carrier.