Methods for promoting production of female embryos in eggs

Abstract

The present invention relates to a method for promoting production of female embryos in fertilized eggs, comprising steps of :incubating said eggs; generating at least one type of energy with a predetermined energy profile; and simultaneously generating a pre-determined profile of a non-energy parameter by means of an energy generation device, applying both energy profile and the non-energy parameter to the eggs; and, collecting the eggs; wherein said method is non-toxic to the embryos, the application of both the energy profile and the non-energy parameter increases the fraction of females in the hatched chicks.

1 . A method for promoting production of female embryos of fertilized eggs, said method comprising steps of:
 a. incubating said eggs;   b. by means of an acoustic waves generation device, generating a predetermined acoustic waves profile;   c. applying said acoustic waves profile to said eggs; and   d. harvesting said eggs;   wherein said method is non-toxic to the embryos, further wherein application of said acoustic profile increases a fraction of females in chicks hatched from the harvested eggs, wherein said energy profile is characterized by altering sound frequency between about 0 Hz to about 1,200 Hz.   
     
     
         2 . The method of  claim 1 , wherein said energy profile is characterized by:
 a. for days 3-15, alternating sound frequency (±50 Hz) wherein said sound frequency equals=0.0032*day 6 −0.1428*day 5 +2.5087*day 4 −22.139*day 3 +104.69*day 2 −255.88*day+866.22; and   b. alternating sound intensity in a range of 19 to 42 dB.   
     
     
         3 . The method of  claim 1 , wherein said energy profile is characterized by:
 a. for days 3-13, alternating sound frequency (±50 Hz) wherein said sound frequency equals−0.0554day 6 +2.6917day 5 −52.609day 4 +527.75day 3 −2850.3day 2+ 7794.1day −7792.3; and   b. alternating sound intensity in a range of 19 to 42 dB.   
     
     
         4 . The method of  claim 1 , wherein at least one parameter of said acoustic waves profile is selected from a group consisting of intensity, duration, frequency of pulses of said acoustic waves, duty cycle, shape of said pulses of said acoustic waves, number of pulses per a group, number of group of pulses, duration of said group, and any combination thereof. 
     
     
         5 . The method of  claim 1 , wherein said acoustic waves profile is continuous or interrupted. 
     
     
         6 . The method of  claim 5 , wherein said shape of said pulse is at least one selected from a group consisting of a sinusoidal wave, a square wave, a saw-tooth wave, sinusoid-like wave, a triangle wave, a descending wave, an elevating wave, a multivariable wave, propagating longitudinal waves comprising alternating compressions and rarefactions and any combination thereof. 
     
     
         7 . The method of  claim 5 , wherein said shape of said group of pulses is at least one selected from a group consisting of a sinusoidal wave, a square wave, a saw-tooth wave, sinusoid-like form, a triangle wave, a decreasing intensity wave, an increasing intensity wave, a multivariable wave and any combination thereof. 
     
     
         8 . The method of  claim 4 , wherein said duration of said group is in a range of 0 hours to 48 hours. 
     
     
         9 . The method of  claim 4 , wherein all of said parameter of said acoustic waves are applied on a day in a range of day 1 to day 14 of incubation. 
     
     
         10 . The method of  claim 1 , wherein an intensity of said acoustic waves is in a range of 0 dB to 40 dB. 
     
     
         11 . The method of  claim 1 , wherein incubating said eggs is conducted at a humidity range of 55% to 85%. 
     
     
         12 . The method of  claim 1 , wherein incubating said eggs is conducted at a temperature in a range of 35.9° C. to 37° C. 
     
     
         13 . The method of  claim 1 , wherein incubating said eggs further comprises tilting the eggs at about 45 degrees once per hour to once per two hours.