Dna vaccine against amyloid-beta and tau

Abstract

An object of the present invention is to provide a vaccine that can simultaneously reduce Aβ deposition and tau deposition in the brain by means of a single molecule. The present invention provides a recombinant vector comprising DNA encoding amyloid-β, DNA encoding an immunoglobulin Fc sequence, and DNA encoding tau.

1 . A polypeptide comprising a fusion protein having a region consisting of (i)-(vi): (i) four (4) repeats of Aβ1-42 or Aβ1-43 sequence, (ii) an immunoglobulin Fc, and (iii) four (4) repeats of tau sequence, (iv) optionally an Ig leader sequence, (v) optionally peptide linker/s, and (vi) optionally amino acid sequence/s encoded by restriction enzyme site/s, wherein the four (4) repeats of tau sequence comprises four (4) repeats of the amino acid sequences as set forth in SEQ ID NO: 22. 
     
     
         2 . The polypeptide according to  claim 1 , wherein the amyloid 13 is Aβ1-42. 
     
     
         3 . The polypeptide according to  claim 1 , wherein the polypeptide does not comprise a portion containing IL-4. 
     
     
         4 . The polypeptide according to  claim 1 , wherein the repeats of Aβ1-42 or Aβ1-43, an immunoglobulin Fc sequence, and repeats of tau sequence of the fusion protein are contained in said order in the fusion protein. 
     
     
         5 . The polypeptide according to  claim 1 , wherein each repeat of Aβ1-42 comprises the amino acid sequence of SEQ ID NO: 8. 
     
     
         6 . The polypeptide according to  claim 1 , wherein the four (4) repeats of tau sequence comprises the amino acid sequence as set forth in SEQ ID NO: 24. 
     
     
         7 . A vaccine for prevention or treatment of Alzheimer's disease, comprising the polypeptide according to  claim 1 . 
     
     
         8 . A vaccine for reducing brain Aβ and brain tau, comprising the polypeptide according to  claim 1 . 
     
     
         9 . An inducer of anti-Aβ antibody and anti-tau antibody, comprising the polypeptide according  claim 1 . 
     
     
         10 . A recombinant vector comprising DNA having a region consisting of (i)-(vi): (i) DNA encoding four (4) repeats of Aβ1-42 or Aβ1-43 sequence, (ii) DNA encoding an immunoglobulin Fc sequence, (iii) DNA encoding four (4) repeats of tau sequence, (iv) optionally DNA encoding an Ig leader sequence, (v) optionally DNA encoding peptide linker/s, and (vi) optionally restriction enzyme sites, wherein the four (4) repeats of tau sequence comprises four (4) repeats of the amino acid sequences as set forth in SEQ ID NO: 22. 
     
     
         11 . A DNA vaccine for prevention or treatment of Alzheimer's disease, comprising the recombinant vector according to  claim 10 . 
     
     
         12 . A DNA vaccine for reducing brain Aβ and brain tau, comprising the recombinant vector according to  claim 10 . 
     
     
         13 . An inducer of anti-Aβ antibody and anti-tau antibody, comprising the recombinant vector according to  claim 10 .