Method for preparing liquid medium composition, and preparation device and kit therefor

Abstract

The present invention provides a method capable of easily mixing any liquid containing a linking substance such as a divalent metal cation and the like with a liquid containing a particular compound at a high concentration, and capable of producing a liquid medium composition comprising fine structures dispersed therein, and a production device therefor and a kit therefor. The first liquid containing a particular compound is passed through a through-hole having a given cross-sectional area formed in a nozzle part at a given flow rate and injected into the second liquid at a given flow rate. By this simple operation, a structure in which the particular compound is bonded via the linking substance is formed, and the structure is preferably dispersed in a mixture of the both liquids.

1 . A kit for carrying out a production method of liquid medium composition, which kit comprises at least a first container, a syringe, and a second container, wherein
 the first container contains a first liquid comprising a particular compound of a particular compound which is a polymer compound having an anionic functional group, and capable of forming a structure by linking via a divalent metal cation, which structure being capable of suspending a cell or a tissue,   the syringe functions as a supply device for feeding out the first liquid, and   the second container contains a second liquid comprising a linking substance which is a divalent metal cation, and   the second container comprises a body and a lid, wherein said body or lid is provided with a nozzle part having a through-hole communicating the outside of the container and the inside of the container, said through-hole has a cross-sectional area of 0.01 mm 2 -5.00 mm 2 , the nozzle part is provided on the lid of the container, a tubular component for fitting a syringe tip protrudes from an outer surface of the lid at a container external side of the nozzle par.   
     
     
         2 . The kit according to  claim 1 , wherein the syringe is further equipped with a tubular component to be inserted in the first container and used to suck the first liquid, said tubular component comprising
 a thin-tube part having an outer diameter permitting insertion into the first container, and a length enabling suction of the first liquid from the first container, and   a connecting part at one end of the thin-tube part, mountable on the tip of the cylinder of the syringe.   
     
     
         3 . The kit according to  claim 1 , wherein the second container is further equipped with a sealing lid free of a nozzle part and configured to be able to seal the inside of the body of the container, and the lid of the second container and the sealing lid can be compatibly mounted on an opening part of the body of the container. 
     
     
         4 . The kit according to  claim 3 , wherein the syringe is further equipped with a tubular component to be inserted in the first container and used to suck the first liquid, said tubular component comprising
 a thin-tube part having an outer diameter permitting insertion into the first container, and a length enabling suction of the first liquid from the first container, and   a connecting part at one end of the thin-tube part, mountable on the tip of the cylinder of the syringe.