Cosmetic appearance of skin

Abstract

The present invention is directed to targeted delivery of a cold slurry underneath a patient's skin to improve the cosmetic appearance of the skin. The “target” tissue can be muscle tissue that when cooled cannot contract and cause wrinkles/lines in the skin to appear. The target tissue can also be adipose tissue that causes overlying tissue to sag and form wrinkles/lines. The cold slurry induces apoptosis, and reduces the size of the tissue, and causes the connective tissue supporting the skin to thicken and tighten, thereby reducing wrinkles/lines. Skin can also be tightened with cold slurry injected into the dermis layer of the skin or within the layers of the oral mucosa making a cold slurry treatment ideal for reducing the appearance of baggy skin after a patient loses weight and or for treating obstructive sleep apnea. Cold slurry can also be used to treat cellulite and acne.

1 - 20 . (canceled) 
     
     
         21 . A method of treating acne, comprising:
 delivering a cold slurry from a delivery device to a target tissue underneath a subject's skin thereby cooling the target tissue;   wherein the target tissue is selected from a group consisting of a sebaceous gland, a nerve that innervates a sebaceous gland, and a combination of a sebaceous gland and a nerve that innervates the sebaceous gland.   
     
     
         22 . The method of  claim 21 , wherein the cold slurry disrupts or destroys the activity of the sebaceous gland. 
     
     
         23 . The method of  claim 21 , wherein the cold slurry disrupts the sebocyte cell membrane. 
     
     
         24 . The method of  claim 21 , wherein the cold slurry reduces sebocyte lipid content. 
     
     
         25 . The method of  claim 21 , wherein the cold slurry disrupts alkaline phosphatase activity. 
     
     
         26 . The method of  claim 21 , wherein the sebaceous gland comprises a new sebaceous gland. 
     
     
         27 . The method of  claim 21 , wherein the sebaceous gland comprises an old sebaceous gland. 
     
     
         28 . The method of  claim 21 , further comprising delivering a cold slurry having a first temperature to disrupt a new sebaceous gland, and optionally delivering a cold slurry having a second temperature to disrupt an old sebaceous gland. 
     
     
         29 . The method of  claim 21 , further comprising maintaining activity of a sebaceous gland. 
     
     
         30 . The method of  claim 21 , further comprising cooling any one of connective tissue, adipose tissue and a combination thereof with the cold slurry, thereby inducing a response that causes the skin to tighten so as to reduce the appearance of wrinkles and lines in the skin. 
     
     
         31 . The method of  claim 21 , further comprising cooling any one of a muscle, a nerve that innervates a muscle, and a combination of a muscle and a nerve that innervates the muscle with the cold slurry temporarily, thereby inhibiting the muscle from contracting so as to reduce the appearance of wrinkles and lines in the skin, which are associated with the muscle contraction. 
     
     
         32 . The method of  claim 21 , further comprising mechanically disrupting fibrous tissue strands in cellulite in the subject to improve a cosmetic appearance of the subject's skin which has a dimpled appearance, wherein the cold slurry comprises ice particles, and wherein the ice particles mechanically disrupt the fibrous tissue strands, thereby reducing the dimpled appearance and improving the appearance of the subject's skin. 
     
     
         33 . The method of  claim 32 , further comprising cooling adipose tissue in cellulite, which has a dimpled appearance, with the cold slurry, thereby inducing apoptosis and reducing the dimpled appearance. 
     
     
         34 . The method of  claim 21 , further comprising delivering the cold slurry to tissue adjacent to the target tissue. 
     
     
         35 . The method of  claim 21 , wherein delivering the cold slurry includes inserting a cannula through the subject's skin, advancing the cannula to the target tissue, and delivering the cold slurry to the target tissue. 
     
     
         36 . The method of  claim 21 , wherein the cold slurry comprises about 1% to about 75% of ice particles by weight. 
     
     
         37 . The method of  claim 21 , wherein the cold slurry comprises water, salt and glycerol. 
     
     
         38 . The method of  claim 21 , wherein the cold slurry comprises 0.1% to about 20% glycerol and 0.1% to about 20% salt. 
     
     
         39 . The method of  claim 21 , wherein the cold slurry has a mean temperature selected from the group consisting of: about +10° C., about +7° C., about +5° C., about +4° C., about +3° C., about +2° C., about +1° C., about 0° C., about −1° C., about −2° C., about −3° C., about −4° C., about −5° C., about −10° C., about −15° C., about −20° C., about −30° C., about −40° C., and about −50° C. 
     
     
         40 . The method of  claim 21 , wherein the cold slurry comprises spherical or round ice particles with a diameter of about 1 mm to about 0.01 mm. 
     
     
         41 . The method of  claim 21 , further comprising introducing a fluid into a lumen of the delivery device; agitating the fluid within the lumen with an integrated agitation device; and cooling the fluid with a cooling device integrated in the delivery device to generate the cold slurry, wherein delivering the cold slurry includes injecting the cold slurry from the delivery device using a plunger at least partially disposed within the lumen. 
     
     
         42 . The method of  claim 21 , wherein the delivery device comprises a cannula; and wherein delivering the cold slurry includes inserting the cannula through the subject's skin, advancing the cannula to the target tissue, and delivering the cold slurry to the target tissue. 
     
     
         43 . The method of  claim 41 , wherein the fluid is agitated while cooling the fluid. 
     
     
         44 . The method of  claim 41 , wherein the cooling device at least partially surrounds the lumen.