Methods for Detecting Symmetrical Dimethylarginine

Abstract

Method of detecting Symmetrical dimethyl arginine (SDMA) in biological samples. SDMA analogs for generating anti-SDMA antibodies having little or no cross-reactivity with asymmetrical dimethyl arginine, arginine, and monomethylarginine. The analogs have a protected or free thiol (—SH) group or hydroxyl (—OH) group that allow them to be linked to a suitable conjugation target which can be, for example, a protein containing molecule of a label. The anti-SDMA antibodies can be used in diagnostic immunoassay for the diagnosis of SDMA associated disorders and/or diseases.

1 . A device for determining the presence of free symmetrical dimethylarginine (SDMA) in a sample, the device comprising an SDMA analog attached to a solid phase. 
     
     
         2 . The device of  claim 1 , further comprising unbound free SDMA from a patient sample on the solid phase. 
     
     
         3 . The device of  claim 2 , wherein the SDMA analog is attached to the solid phase through a carboxyl group (—CO(OH)) on SDMA. 
     
     
         4 . The device of  claim 3 , wherein a hydroxyl (OH) group of the carboxyl group (—CO(OH)) on SDMA is converted to an amide bond (—CO(NH—)) when conjugated. 
     
     
         5 . The device of  claim 1 , wherein the SDMA analog is selected from the group consisting of: 
       
         
           
           
               
               
           
         
       
       wherein x and y are integers ranging from 1 to 5.