Carrier-binding agent compositions and methods of making and using the same

Abstract

Described herein are compositions of binding agents and carrier proteins, and optionally at least one therapeutic agent, and methods of making and using the same, in particular, as a cancer therapeutic. Also described are lyophilized compositions of binding agents and carrier proteins, and optionally at least one therapeutic agent, and methods of making and using the same, in particular, as a cancer therapeutic.

1 - 38 . (canceled) 
     
     
         39 . A lyophilized nanoparticle composition comprising nanoparticle complexes having an outer surface, wherein each of the nanoparticle complexes comprises:
 a) between about 100 to about 1000 binding agents arranged on the outer surface, wherein each binding agent comprises an anti-VEGF antibody, or an antigen-binding portion thereof;   b) a therapeutically effective amount of paclitaxel; and   c) albumin, wherein the albumin is non-covalently bound to paclitaxel and the binding agents;   wherein each of the nanoparticle complexes is configured such that in response to reconstitution with an aqueous solution the binding agent remains arranged on the outer surface and the anti-VEGF antibody, or antigen-binding portion thereof, of said binding agent remains capable of binding to VEGF in vivo, and wherein fewer than about 50% of said nanoparticle complexes are oligomeric.   
     
     
         40 . The lyophilized nanoparticle composition of  claim 39 , wherein the composition is stable at about 20° C. to about 25° C. for up to about 12 months or longer. 
     
     
         41 . The lyophilized nanoparticle composition of  claim 39 , wherein less than 40% of the nanoparticle complexes present in said composition are oligomerized. 
     
     
         42 . The lyophilized nanoparticle composition of  claim 39 , wherein less than 30% of the nanoparticle complexes present in said composition are oligomerized. 
     
     
         43 . The lyophilized nanoparticle composition of  claim 39 , wherein less than 20% of the nanoparticle complexes present in said composition are oligomerized. 
     
     
         44 . The lyophilized nanoparticle composition of  claim 39 , wherein less than 10% of the nanoparticle complexes present in said composition are oligomerized. 
     
     
         45 . The lyophilized nanoparticle composition of  claim 39 , wherein less than 5% of the nanoparticle complexes present in said composition are oligomerized. 
     
     
         46 . The lyophilized nanoparticle composition of  claim 39 , wherein the average size of the nanoparticle complexes is between 130 nm and 800 nm. 
     
     
         47 . The lyophilized nanoparticle composition of  claim 39 , wherein said nanoparticle complexes have an average size of approximately 160 nm. 
     
     
         48 . The lyophilized nanoparticle composition of  claim 39 , wherein the binding agent comprises bevacizumab. 
     
     
         49 . The lyophilized nanoparticle composition of  claim 39 , wherein the albumin is human serum albumin. 
     
     
         50 . The lyophilized nanoparticle composition of  claim 39 , wherein the albumin is recombinant human serum albumin. 
     
     
         51 . The lyophilized nanoparticle composition of  claim 39 , wherein upon reconstitution the composition is formulated for intravenous delivery. 
     
     
         52 . The lyophilized nanoparticle composition of  claim 51 , wherein upon reconstitution, the composition is formulated for direct injection or perfusion into a tumor. 
     
     
         53 . The lyophilized nanoparticle composition of  claim 47 , wherein a dissociation constant of albumin-bound paclitaxel and binding agent is between about 1×10 −11  M and about 1×10 −9  M. 
     
     
         54 . A method for killing viable cancer cells in a population of cancer cells, the method comprising reconstituting the nanoparticle composition of  claim 39  with an aqueous solution, and contacting the cells with a therapeutically effective amount of the reconstituted nanoparticle composition. 
     
     
         55 . The method of  claim 54 , wherein the therapeutically effective amount of the nanoparticle composition comprises about 75 mg/m 2  to about 175 mg/m 2  paclitaxel. 
     
     
         56 . The method of  claim 54 , wherein the therapeutically effective amount of the nanoparticle composition comprises about 30 mg/m 2  to about 70 mg/m 2  bevacizumab.