Method for Processing Blood Sample

Abstract

A method for processing a blood sample is provided that can improve the recovery rate of deformable rare cells that would easily pass through a filter and small rare cells while reducing the filtration area of the filter, and that can recover the rare cells alive. In one aspect, the present disclosure relates to a method for separating or detecting rare cells in a blood sample using a filter including holes with a hole density of at least 200 holes/mm 2 to 40000 holes/mm 2 , wherein the holes are in the form of an ellipse with a minor axis diameter of 3.0 μm to 15 μm and a major axis diameter of 1.1 times to 3 times as long as the minor axis diameter, or the holes have a shape that contains the ellipse and is in contact with the ellipse on at least two points including both ends of the major axis of the ellipse. The method includes separating or detecting rare cells by filtering the blood sample in a volume of 6 μl/hole or less with respect to a hole of the filter.

1 - 24 . (canceled) 
     
     
         25 . A filter for separating or detecting rare cells in a blood sample comprising holes with a hole density of 200 holes/mm 2  to 40000 holes/mm 2 , wherein the holes are in the form of an ellipse with a minor axis diameter of 3.0 μm to 15 μm and a major axis diameter of 1.1 times to 3 times as long as the minor axis diameter, or the holes have a shape that contains the ellipse and is in contact with the ellipse on at least two points including both ends of the major axis of the ellipse. 
     
     
         26 . The filter according to  claim 25 , comprising holes with a hole density of at least 200 holes/mm 2  to 40000 holes/mm 2 , wherein the holes are in the form of an ellipse with a minor axis diameter of 3.0 μm to 15 μm and a major axis diameter of 1.2 times to 3 times as long as the minor axis diameter, or the holes have a shape that is in contact with the ellipse on at least two points including both ends of the major axis of the ellipse. 
     
     
         27 . The filter according to  claim 25 , comprising more than 10000 holes, wherein the filter allows 1 ml to 5 ml of blood to be processed. 
     
     
         28 . The filter according to  claim 25 , comprising 29000 holes or more, wherein the filter allows 5 ml to 10 ml of blood to be processed. 
     
     
         29 . The filter according to  claim 25 , comprising 60000 holes or more, wherein the filter allows more than 10 ml of blood to be processed. 
     
     
         30 . A device for capturing rare cells that captures rare cells in a sample, comprising: an inlet; an outlet; and a flow path connecting the inlet and the outlet,
 wherein a separation portion that includes the filter according to  claim 25  and a filter holder is provided in a position corresponding to a part of the flow path.