Universal blood product and methods of preparing and using same

Abstract

A method of preparing a universal blood product comprising obtaining a blood product; contacting the blood product with (i) hydroxyapatite; (ii) a carbonaceous material comprising at least a mixture of a first carbon particle having macroporous size α and a second carbon particle having macroporous size β; and (iii) at least one support matrix chemically associated with an antigenic determinant.to form a cleansed product; and recovering the cleansed product.

What is claimed is: 
     
         1 . A method of preparing a universal blood product comprising:
 obtaining a blood product wherein the blood product comprises whole blood or plasma;   contacting the blood product with (i) hydroxyapatite; (ii) a carbonaceous material comprising at least a mixture of a first carbon particle having macroporous size α and a second carbon particle having macroporous size β; and (iii) at least one support matrix chemically associated with an antigenic determinant.to form a cleansed product; and   recovering the cleansed product.   
     
     
         2 . The method of  claim 1  wherein the hydroxyapatite is naturally-occurring, synthetic, or combinations thereof. 
     
     
         3 . The method of  claim 1  wherein the hydroxyapatite has a Brunauer Emmett Teller surface area of from about 200 m 2 /g to about 3000 m 2 /g. 
     
     
         4 . The method of  claim 1  wherein the hydroxyapatite is in the form of beads having a particle size of from about 300 μm to about 1000 μm. 
     
     
         5 . The method of  claim 1  wherein the hydroxyapatite has a unimodal pore size distribution with a pore size ranging from about 2 nm to greater than about 1000 nm. 
     
     
         6 . The method of  claim 1  wherein macroporous size α ranges from about 125 nm to about 250 nm. 
     
     
         7 . The method of  claim 1  wherein macroporous size β ranges from about 250 nm to about 500 nm. 
     
     
         8 . The method of  claim 1  wherein the carbonaceous material further comprises micropores having a pore size range of from about 2 nm to about 50 nm. 
     
     
         9 . The method of  claim 1  wherein the immobilized antigenic determinant comprises a Blood Group A determinant, a Blood Group B determinant, or combinations thereof. 
     
     
         10 . The method of  claim 9  wherein the Blood Group A determinant comprises A-trisaccharides α-D-GalNAc-(1→3)-(α-L-Fuc-[1→2])-D-Gal. 
     
     
         11 . The method of  claim 9  wherein the Blood Group B determinant comprises B-trisaccharides α-D-Gal-(1→3)-(α-L-Fuc-[1→2])-D-Gal. 
     
     
         12 . The method of  claim 1  wherein the support matrix with chemically associated antigenic determinant is immobilized on a support matrix selected from the group consisting of polyethylene (PE), sepharose; silica; polyoxymethylene (POM), polypropylene (PP), polyvinylchloride (PVC), polyvinyl acetate (PVA), polyvinylidene chloride (PVDC), polystyrene (PS), polytetrafluoroethylene (PTFE), polyacrylate, poly(methyl methacrylate) (PMMA), polyacrylamide, polyglycidyl methacrylate (PGMA), acrylonitrile butadiene styrene (ABS), polyacrylonitrile (PAN), polyester, polycarbonate, polyethylene terephthalate (PET), polyamide, polyaramide, polyethylene glycol (PEG), polyvinylpyrrolidone (PVP), polysulfone (PS), polyethersulfone (PES), polyarylethersulfone (PEAS), ethylene vinyl acetate (EVA), ethylene vinyl alcohol (EVOH), polyamide-imide, polyaryletherketone (PAEK), polybutadiene (PBD), polybutylene (PB), polybutylene terephthalate (PBT), polycaprolactone (PCL), polyhydroxyalkanoate, polyether ether ketone (PEEK), polyether ketone ketone (PEKK), polyether imide (PEI), polyimide, polylactic acid (PLA), polymethyl pentene (PMP), poly(p-phenylene ether) (PPE), polyurethane (PU), styrene acrylonitrile (SAN), polybutenoic acid, poly(4-allyl-benzoic acid), poly(glycidyl acrylate), polyglycidyl methacrylate (PGMA), poly(allyl glycidyl ether), poly(vinyl glycidyl ether), poly(vinyl glycidyl urethane), polyallylamine, polyvinylamine, copolymers of said polymers; derivatives of said polymers and combinations thereof. 
     
     
         13 . The method of  claim 12  wherein the support matrix comprise a polysaccharide selected from the group consisting of cellulose, nitrocellulose, chitosan, collagen, starch, cross-linked polysaccharide gels car combinations thereof. 
     
     
         14 . The method of  claim 12  wherein the support matrix is in the form of a bead, a sheet, or a hollow fiber membrane. 
     
     
         15 . The method of  claim 1  further comprising a linker group coupled with both the support matrix and the support matrix with chemically associated antigenic determinant. 
     
     
         16 . The method of  claim 15  wherein the linker group comprises an amino acid. 
     
     
         17 . The method of  claim 1  wherein the cleansed product has an amount of at least one molecule selected from the group consisting of TNF-α, IL-1β, IL-4, IL-6, IL-8, IL-10, IFN-γ, and TGF-β that is reduced by from about 10% to about 50% when compared to the amount present in the blood product. 
     
     
         18 . The method of  claim 1  wherein the blood product is obtained from at least two subjects. 
     
     
         19 . A hand held device comprising (i) hydroxyapatite; (ii) a carbonaceous material and (iii) a hollow fiber membrane containing at least one support matrix with chemically associated antigenic determinant. 
     
     
         20 . A method of preparing a universal blood product comprising:
 obtaining a blood product wherein the blood product comprises whole blood or plasma;   contacting the blood product with (i) hydroxyapatite; (ii) a carbonaceous material comprising at least a mixture of a first carbon particle having macroporous size α and a second carbon particle having macroporous size β; and (iii) at least one support matrix chemically associated with an antigenic determinant.to form a cleansed product; wherein at least one of the hydroxyapatite, carbonaceous material and support matrix is functionalized.