Feed additive formulation and methods of making and using the same

Abstract

The presently disclosed subject matter relates to feed additive formulations for monogastric animal feed. Particularly, the disclosed formulations comprise an isolated xylanase enzyme and a B. licheniformis strain PWD-1. The feed additive formulations may further include B. amyloliquefaciens strain Ba-BPD1. The disclosed formulations are useful for addition to feeds for monogastric animals to synergistically improve the performance of the animals.

What is claimed is: 
     
         1 . A feed additive formulation for monogastric animal feed, the formulation comprising an isolated xylanase enzyme and a biologically pure culture of a  Bacillus licheniformis  strain PWD-1 (Accession No. 53757), or a mutant thereof having all the identifying characteristics thereof. 
     
     
         2 . The feed additive formulation of  claim 1 , further comprising a biologically pure culture of a  Bacillus amyloliquefaciens  strain Ba-BPD1 (Accession No. DSM 21836), or a mutant thereof having all the identifying characteristics thereof. 
     
     
         3 . The feed additive formulation of  claim 1 , wherein the xylanase is an endo-1,4-beta-xylanase. 
     
     
         4 . The feed additive formulation of  claim 1 , wherein the formulation is a solid, a powder, a suspension concentrate, a liquid, or a granule. 
     
     
         5 . The feed additive formulation of  claim 1 , further comprising a carrier. 
     
     
         6 . The feed additive formulation of  claim 5 , wherein the carrier comprises limestone. 
     
     
         7 . The feed additive formulation of  claim 1 , wherein the xylanase ranges from 10,000-200,000 units/gram and the  Bacillus licheniformis  ranges from 10 8 -10 12  CFU/gram. 
     
     
         8 . The feed additive formulation of  claim 2 , wherein the xylanase ranges from 10,000-200,000 units/gram, the  Bacillus licheniformis  ranges from 10 8 -10 12  CFU/gram, and the  Bacillus amyloliquefaciens  ranges from 10 8 -10 12  CFU/gram. 
     
     
         9 . A feed composition for monogastric animals comprising the feed additive formulation of  claim 1 . 
     
     
         10 . The feed composition of  claim 9 , further comprising a biologically pure culture of a  Bacillus amyloliquefaciens  strain Ba-BPD1 (Accession No. DSM 21836), or a mutant thereof having all the identifying characteristics thereof. 
     
     
         11 . The feed composition of  claim 9 , wherein the xylanase ranges from 5-30 units/gram feed and the  Bacillus licheniformis  ranges from at least 5×10 4  CFU/gram feed. 
     
     
         12 . The feed composition of  claim 10 , wherein the xylanase ranges from 5-30 units/gram feed and each of the  Bacillus licheniformis  and the  Bacillus amyloliquefaciens  ranges from at least 10 5  CFU/gram feed for a total of at least 1×10 5  CFU/gram feed. 
     
     
         13 . A method for increasing the performance of a monogastric animal comprising:
 administering to the monogastric animal an effective amount of a feed composition comprising a xylanase enzyme and a biologically pure culture of a  Bacillus licheniformis  strain PWD-1 (Accession No. 53757), or a mutant thereof having all the identifying characteristics thereof.   
     
     
         14 . The method of  claim 13 , wherein the feed composition further comprises a biologically pure culture of a  Bacillus amyloliquefaciens  strain Ba-BPD1 (Accession No. DSM 21836), or a mutant thereof having all the identifying characteristics thereof. 
     
     
         15 . The method of  claim 13 , wherein the xylanase is an endo-1,4-beta-xylanase. 
     
     
         16 . The method of  claim 13 , wherein the animal is poultry. 
     
     
         17 . The method of  claim 16 , wherein the animal is a broiler. 
     
     
         18 . The method of  claim 13 , wherein the animal is swine. 
     
     
         19 . The method of  claim 13 , wherein the increase in performance comprises one or a combination of: increased average daily weight gain, increased total weight gain, improved feed conversion rate, reduced lesion score, reduced mortality, reduced disease, or decreased pathogen incidence. 
     
     
         20 . The method of  claim 19 , wherein one or both of the disease and lesion score is a result of one or a combination of  Clostridium perfringens, Eimeria acervulina, Eimeria maxima, Eimeria tenella  or Coccidiosis-inducing parasites. 
     
     
         21 . The method of  claim 19 , wherein the animal is poultry and the increase in performance comprises reduced lesion score resulting from one or a combination of  Clostridium perfringens  or  Eimeria  spc. 
     
     
         22 . The method of  claim 19 , wherein the animal is poultry and the decreased pathogen incidence is a decreased  Salmonella  incidence. 
     
     
         23 . A method of preparing a feed composition for monogastric animals, comprising adding to the feed composition a formulation comprising a xylanase enzyme and a biologically pure culture of a  Bacillus licheniformis  strain PWD-1 (Accession No. 53757), or a mutant thereof having all the identifying characteristics thereof. 
     
     
         24 . The method of  claim 23 , wherein the formulation further comprises a biologically pure culture of a  Bacillus amyloliquefaciens  strain Ba-BPD1 (Accession No. DSM 21836), or a mutant thereof having all the identifying characteristics thereof. 
     
     
         25 . The method of  claim 23 , wherein the xylanase is an endo-1,4-beta-xylanase. 
     
     
         26 . The method of  claim 23 , wherein the formulation is a solid, a powder, a suspension concentrate, a liquid, or a granule. 
     
     
         27 . The method of  claim 23 , wherein the xylanase ranges from 5-30 units/gram feed and the  Bacillus licheniformis  ranges from at least 5×10 4  CFU/gram feed. 
     
     
         28 . The method of  claim 24 , wherein the xylanase ranges from 5-30 units/gram feed and each of the  Bacillus licheniformis  and the  Bacillus amyloliquefaciens  ranges from at least 10 5  CFU/gram feed for a total of at least 1×10 5  CFU/gram feed.