Immunochromatography analysis device for detecting dengue virus

Abstract

The invention relates to an immunochromatography analysis device which enables simple and rapid diagnosis of dengue virus infection, and an object thereof is to provide an immunochromatography analysis device which can reduce a cross-reaction with a virus belonging to Flaviviridae other than dengue virus and which can specifically detect dengue virus. The invention relates to an immunochromatography analysis device for detecting dengue virus including a sample application part, a labeling substance-holding part, a chromatography medium part having a detection part and an absorption part, wherein the labeling substance-holding part contains a first antibody which recognizes the amino acid sequence of SEQ ID NO: 2 that is present in the whole amino acid sequence of dengue virus NS1 of SEQ ID NO: 1, and the detection part contains a second antibody which recognizes the three-dimensional structure of dengue virus NS1.

1 . An immunochromatography analysis device for detecting dengue virus in an analyte, including a sample application part, a labeling substance-holding part, a chromatography medium part having a detection part and an absorption part,
 wherein the labeling substance-holding part contains a first antibody which recognizes the amino acid sequence of SEQ ID NO: 2 that is present in the whole amino acid sequence of dengue virus NS1 of SEQ ID NO: 1, and   the detection part contains a second antibody which recognizes the three-dimensional structure of dengue virus NS1.   
     
     
         2 . The immunochromatography analysis device according to  claim 1 , wherein the detection part further contains an antibody which recognizes the amino acid sequence of SEQ ID NO: 2 that is present in the whole amino acid sequence of dengue virus NS1 of SEQ ID NO: 1. 
     
     
         3 . An immunochromatography analysis kit including the immunochromatography analysis device according to  claim 1  and an analyte dilution solution for diluting and developing the analyte. 
     
     
         4 . An immunochromatography analysis method for detecting dengue virus in an analyte using the immunochromatography analysis kit according to  claim 3 , wherein the immunochromatography analysis method includes the following steps (1) to (4),
 (1) a step of applying an analyte-containing solution obtained by diluting the analyte with the analyte dilution solution as a sample to the sample application part,   (2) a step of causing the first antibody which recognizes the amino acid sequence of SEQ ID NO: 2 that is present in the whole amino acid sequence of dengue virus NS1 of SEQ ID NO: 1 and which is held in the labeling substance-holding part to recognize dengue virus in the analyte,   (3) a step of developing the analyte and the first antibody as a mobile phase on the chromatography medium part, and   (4) a step of detecting the dengue virus in the developed mobile phase with the second antibody which recognizes the three-dimensional structure of dengue virus NS1 contained in the detection part.