Reagent kit for lc-ms analysis of aromatic compounds

Abstract

A method for analyzing aromatic compounds, and a reagent kit for LC-MS analysis of aromatic compounds. The method includes preparing a diazonium reagent, contacting aromatic compounds in a sample with the diazonium reagent to form an analyte; and measuring an amount or ratio of the analyte. The reagent kit includes a diazonium reagent, wherein the diazonium reagent includes (i) a diazonium salt that contains a diazonium ion; (ii) an amine and nitrous acid; and/or (iii) a nitrite and an acid.

What is claimed is: 
     
         1 . A reagent kit for LC-MS analysis of aromatic compounds, comprising a diazonium reagent, wherein the diazonium reagent is contacted with the aromatic compounds in a sample to form a first analyte, then by measuring an amount or ratio of the first analyte to the sample that has been formed by liquid chromatography-mass spectrometry analyzing, presence or quantity of the aromatic compounds in the sample is extrapolated;
 wherein the diazonium reagent contains one or more functional groups that can be negatively or positively charged during liquid chromatography-mass spectrometry analyzing; the functional groups can be negatively charged in the diazonium reagent comprise one or more of sulfonate group (—SO 3   − ), carboxylate group (—COO − ), sulfonic acid group (—SO 3 H) and carboxylic acid group (—COOH), and the functional groups that can be positively charged in the diazonium reagent include one or more of quaternary ammonium group, amino group and thiol group;   wherein the diazonium reagent is p-sulfanilic acid diazonium salt solution, Fast Red RC Diazonium Salt or Fast Red TR Diazonium Salt;   wherein when the diazonium reagent is p-sulfanilic acid diazonium salt solution, the aromatic compounds in the sample is Estriol or Estradiol; and   wherein when the diazonium reagent is Fast Red RC Diazonium Salt or Fast Red TR Diazonium Salt, the aromatic compounds in the sample is cannabidiol compounds, Estriol or Estradiol.   
     
     
         2 . The reagent kit of  claim 1 , wherein the diazonium reagent contains one or more aromatic functional groups. 
     
     
         3 . The reagent kit of  claim 1 , wherein the diazonium reagent contains one or more phenyl groups. 
     
     
         4 . The reagent kit of  claim 1 , further comprising one or more items selected from the group consisting of sample extraction solvent, sample reconstitution solvent, sample diluent, sample precipitant, sample elution solvent, internal standards, calibrators, and quencher to quench unreacted diazonium reagent. 
     
     
         5 . The reagent kit of  claim 1 , further comprising a quencher for quenching the unreacted diazonium reagent by reacting with —N 2+ , and wherein the quencher is an aromatic compound containing one or more electron-donating groups. 
     
     
         6 . The reagent kit of  claim 5 , wherein the quencher includes one or more material selected from the group consisting of ascorbic acid, organic halides, and aliphatic compounds with active methylene groups. 
     
     
         7 . The reagent kit of  claim 5 , wherein the quencher includes one or more material selected from the group consisting of phenol, phenol derivatives, naphthol, naphthol derivatives, and aniline derivatives. 
     
     
         8 . The reagent kit of  claim 5 , wherein the quencher is phenol. 
     
     
         9 . The reagent kit of  claim 1 , wherein the diazonium reagent is p-sulfanilic acid diazonium salt solution. 
     
     
         10 . The reagent kit of  claim 1 , wherein the diazonium reagent is Fast Red RC Diazonium Salt or Fast Red TR Diazonium Salt.