Thermosporothrix hazakensis-derived antibacterially active substance

Abstract

This invention provides a novel compound having an antibacterial activity, and a method for producing the same. The compound is represented by Formula (I): or a salt or ester thereof.

1 . A compound represented by Formula (I): 
       
         
           
           
               
               
           
         
       
       or a salt or ester thereof. 
     
     
         2 . A compound having an absorption maximum at 340 nm in its UV spectrum, and a molar absorption coefficient of 30630;
 having a molecular formula of C 74 H 58 Cl 8 O 26  as determined by analyses of an ESI/TOF/MS spectrum and a high-resolution mass spectrum;   showing signals at δ11.49, 10.92, 6.67, 6.41, 6.21, 6.00, 5.91, 4.95, 4.26, 3.96, 3.95, 3.79, 3.71, 3.70, 2.69, 2.19, and 0.90 in a  1 H NMR spectrum (DMSO-d 6 ); and   showing signals at δ188.61, 184.49, 162.20, 159.96, 158.11, 155.76, 155.25, 150.91, 140.73, 140.25, 140.25, 138.20, 137.79, 137.26, 134.60, 133.53, 132.77, 125.42, 121.51, 120.24, 119.19, 114.71, 111.89, 109.54, 102.99, 99.78, 98.55, 77.15, 72.75, 70.21, 67.00, 61.20, 60.68, 56.94, 17.83, 15.90, and 14.96 in a  13 C NMR spectrum (DMSO-d 6 );   or a salt or ester thereof.   
     
     
         3 . The compound, or a salt or ester thereof according to  claim 2 , obtained by culturing  Thermosporothrix hazakensis  in a culture medium, and recovering the compound, or a salt or ester thereof from the culture product. 
     
     
         4 . The compound, or a salt or ester thereof according to  claim 3 , wherein the compound, or a salt or ester thereof is recovered by treating an ethyl acetate extract with a silica gel column, the ethyl acetate extract being obtained by partitioning an aqueous acetone solution extract of the culture product between ethyl acetate and water. 
     
     
         5 . The compound, or a salt or ester thereof according to  claim 3 , wherein  Thermosporothrix hazakensis  is a  Thermosporothrix hazakensis  SK20-1 T  strain. 
     
     
         6 . A method of producing the compound, or a salt or ester thereof according to  claim 1 , comprising a step of culturing  Thermosporothrix hazakensis  in a culture medium, and recovering the compound, or a salt or ester thereof from the culture product. 
     
     
         7 . The method according to  claim 6 , wherein the compound, or a salt or ester thereof is recovered by treating an ethyl acetate extract with a silica gel column, the ethyl acetate extract being obtained by partitioning an aqueous acetone solution extract of the culture product between ethyl acetate and water. 
     
     
         8 . The method according to  claim 6 , wherein  Thermosporothrix hazakensis  is a  Thermosporothrix hazakensis  SK20-1 T  strain. 
     
     
         9 . An antibacterial agent comprising the compound, or a salt or ester thereof according to  claim 1 , as an active ingredient. 
     
     
         10 . An antibacterial agent comprising the compound, or a salt or ester thereof according to  claim 2 , as an active ingredient. 
     
     
         11 . A method of inhibiting bacterial growth, comprising contacting a bacteria with an effective amount of the compound, or a salt or ester thereof of  claim 1 . 
     
     
         12 . A method of inhibiting bacterial growth, comprising contacting a bacteria with an effective amount of the compound, or a salt or ester thereof of  claim 2 . 
     
     
         13 . The method of  claim 11 , wherein the bacteria is selected from the group consisting of:  Geobacillus stearothermophilus, Bacillus subtilis, Streptomyces griseus, Escherichia coli, Thermosporothrix hazakensis, Thermosporothrix narukonensis , and  Streptomyces  sp. 
     
     
         14 . The method of  claim 12 , wherein the bacteria is selected from the group consisting of:  Geobacillus stearothermophilus, Bacillus subtilis, Streptomyces griseus, Escherichia coli, Thermosporothrix hazakensis, Thermosporothrix narukonensis , and  Streptomyces  sp.