Compositions of hetero- and homofermentative lactic acid bacterial species for dual purpose silage preservation

Abstract

The present invention relates to a silage inoculant consisting essentially of a) at least one obligatory heterofermentative lactic acid bacterial species or strain and b) at least one homofermentative bacterial species or strain which (i) does not reduce growth of a), and (ii) reduces pH fast without producing an excess amount of lactic acid. Further, the invention relates to a method for producing a fermented feed product, said method comprising inoculating a plant material with the silage inoculant according to the invention. It has surprisingly been found that the silage inoculant is effective even if the silage has only been incubated for a period of up to 2 or up to 4 days.

1 . A silage inoculant consisting essentially of:
 (a) at least one obligatory heterofermentative lactic acid bacterial strain selected from at least one of  Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus fermentum , and  Lactobacillus reuteri , and   (b) at least one homofermentative bacterial or strain which reduces pH fast without producing an excess amount of lactic acid and is selected from at least one  Enterococcus faecium  and  Lactococcus lactis , wherein the silage inoculant does not include  Lactobacillus plantarum.      
     
     
         2 . (canceled) 
     
     
         3 . The silage inoculant according to  claim 1 , wherein the at least one obligatory heterofermentative lactic acid bacterial species comprises a  Lactobacillus buchneri  strain. 
     
     
         4 . The silage inoculant according to  claim 3 , wherein the  Lactobacillus buchneri  strain comprises the  Lactobacillus buchneri  strain deposited with the Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ) as DSM 22501. 
     
     
         5 . The silage inoculant according to  claim 1 , wherein the at least one homofermentative bacterial strain comprises an  Enterococcus faecium  strain. 
     
     
         6 . (canceled) 
     
     
         7 . The silage inoculant according to  claim 1 , wherein the at least one homofermentative bacterial strain comprises a  Lactococcus lactis  strain. 
     
     
         8 . (canceled) 
     
     
         9 . The silage inoculant according to  claim 7  wherein the  Lactococcus lactis  strain comprises the  Lactococcus lactis  strain deposited at DSMZ as DSM 11037. 
     
     
         10 . The silage inoculant according to  claim 1 , consisting essentially of a  Lactobacillus buchneri  strain and a  Lactococcus lactis  strain. 
     
     
         11 - 15 . (canceled) 
     
     
         16 . The silage inoculant according to  claim 1 , consisting essentially of the  Lactobacillus buchneri  strain deposited at DSMZ as DSM 22501 and the  Lactococcus lactis  strain deposited at DSMZ as DSM 11037. 
     
     
         17 . The silage inoculant according to  claim 1 , wherein the silage inoculant consists essentially of a  Lactobacillus buchneri  strain, a  Lactococcus lactis  strain and an  Enterococcus faecium  strain. 
     
     
         18 . The silage inoculant according to  claim 1 , wherein the at least one obligatory heterofermentative lactic acid bacterial strain comprises a  Lactobacillus brevis  strain. 
     
     
         19 . The silage inoculant according to  claim 1 , wherein the silage inoculant consists essentially of a  Lactobacillus brevis  strain and an  Enterococcus faecium  strain. 
     
     
         20 . The silage inoculant according to  claim 1 , wherein the silage inoculant consists essentially of a  Lactobacillus brevis  strain and a  Lactococcus lactis  strain. 
     
     
         21 . The silage inoculant according to  claim 1 , wherein the silage inoculant consists essentially of a  Lactobacillus brevis  strain, a  Lactococcus lactis  strain, and an  Enterococcus faecium  strain. 
     
     
         22 . The silage inoculant according to  claim 1 , wherein the at least one obligatory heterofermentative lactic acid bacterial strain comprises a  Lactobacillus fermentum  strain. 
     
     
         23 . The silage inoculant according to  claim 1 , wherein the silage inoculant consists essentially of a  Lactobacillus fermentum  strain and an  Enterococcus faecium  strain. 
     
     
         24 . The silage inoculant according to  claim 1 , wherein the silage inoculant consists essentially of a  Lactobacillus fermentum  strain and a  Lactococcus lactis  strain. 
     
     
         25 . The silage inoculant according to  claim 1 , wherein the silage inoculant consists essentially of a  Lactobacillus fermentum  strain, a  Lactococcus lactis  strain, and an  Enterococcus faecium  strain. 
     
     
         26 . The silage inoculant according to  claim 1 , wherein the at least one obligatory heterofermentative lactic acid bacterial strain comprises a  Lactobacillus reuteri  strain. 
     
     
         27 . The silage inoculant according to  claim 1 , wherein the silage inoculant consists essentially of a  Lactobacillus reuteri  strain and an  Enterococcus faecium  strain. 
     
     
         28 . The silage inoculant according to  claim 1 , wherein the silage inoculant consists essentially of a  Lactobacillus reuteri  strain and a  Lactococcus lactis  strain. 
     
     
         29 . The silage inoculant according to  claim 1 , wherein the silage inoculant consists essentially of a  Lactobacillus reuteri  strain, a  Lactococcus lactis  strain, and an  Enterococcus faecium  strain. 
     
     
         30 . The silage inoculant according to  claim 1 , wherein the homofermentative strain does not produce nisin. 
     
     
         31 . The silage inoculant according to  claim 1 , wherein the homofermentative strain inhibits growth of the obligatory heterofermentative strain by less than 5 mm when the homofermentative strain and the obligatory heterofermentative strain are streaked onto the same Mann-Rogosa-Sharpe (MRS) agar plate and incubated overnight under anaerobic conditions at 37° C. 
     
     
         32 . The silage inoculant according to  claim 1 , wherein the homofermentative strain produces not more than 3 mg/ml lactic acid after 24 hours inoculation at 150,000 CFU/ml into 10 ml sterile silage medium contained in a tube held in a water bath at 30° C., wherein the sterile silage medium is produced by mixing 5 g/L yeast extract, 5 g/L peptone soya neutralized, 0.8 g/L soluble starch, 0.08 g/L manganese(II) sulfate dihydrate, 0.037 g/L succinic acid, 0.069 g/L citric acid monohydrate, and 0.14 L-malic acid in 900 mL water until dissolved, adjusting the pH to 6.3, autoclaving in bottles at 121° C. for 15 minutes, and then adding 100 ml of sterile filtered sugar solution containing 56 g/L D(−) fructose, 32 g/L D(+) glucose monohydrate, 20 g/L D(+) xylose, 20 g/L L(+) arabinose, and 32 g/L sucrose.