Detection component for blood group antigens

Abstract

A detection component for blood group antigens comprising a test strip and a sample addition device, and a sample is transported to a testing section of the test strip by a sampling ring of the sample addition device. Immunochromatography technology is used to identify human blood group antigens with existing blood group antibodies. The antibodies are pre-coated with and solidified on the reaction membrane to define testing points and then assembled to the flushing pad, and the water absorption paper and the pad to define the test strip.

What is claimed is: 
     
         1 . A detection component for blood group antigens, comprising:
 a test strip comprising a base board, wherein:
 a reaction membrane, a flushing pad, a water absorption pad, and a sealing pad are disposed on the base board, 
 the reaction membrane is disposed on a middle portion of the base board, 
 one end of the flushing pad overlaps one end of the reaction membrane, 
 one end of the water absorption pad overlaps another end of the reaction membrane, 
 one part of the sealing pad is stuck to the reaction membrane, 
 another part of sealing pad is stuck to the flushing pad, 
 a section of the reaction membrane disposed between one end of the sealing pad and the one end of the water absorption pad defines a testing section, 
 an A testing point, a B testing point, a D testing point, and a Ctl testing point are disposed on the testing section, 
 the A testing point is coated with a solidified anti-A monoclonal antibody, 
 the B testing point is coated with a solidified anti-B monoclonal antibody, 
 the D testing point is coated with a solidified anti-D monoclonal antibody, and 
 the Ctl testing point is coated with a solidified anti-RBC monoclonal antibody; and 
   a sample addition device comprising a handle and a sampling ring, wherein:
 the sampling ring is disposed on one end of the handle, 
 a length of the sampling ring is less than a width of the reaction membrane and is sufficient to cover the A testing point, the B testing point, the D testing point, and the Ctl testing point, 
 an inner side wall of the sampling ring comprises a plurality of protrusions equally spaced along a circumference of the inner side wall of the sampling ring to increase a surface tension force of the sampling ring and to ensure a sampling volume of 10-20 μL, and 
 a sample is transported to the testing section of the test strip by the sampling ring of the sample addition device. 
   
     
     
         2 . The detection component for the blood group antigens according to  claim 1 , wherein a tag is disposed on the water absorption pad. 
     
     
         3 . The detection component for the blood group antigens according to  claim 1 , wherein the A testing point, the B testing point, the D testing point, and the Ctl testing point are linearly arrayed or staggered up and down to be shaped as an “N” along a width direction of the testing section. 
     
     
         4 . The detection component for the blood group antigens according to  claim 1 , wherein the sampling ring is shaped as an ellipse or a rectangle. 
     
     
         5 . The detection component for the blood group antigens according to  claim 1 , wherein the sampling ring is shaped as a rectangle having a circular angle. 
     
     
         6 . The detection component for the blood group antigens according to  claim 1 , wherein the reaction membrane is a nitrocellulose membrane, a cellulose acetate membrane, a polyethylene membrane, or a nylon membrane. 
     
     
         7 . The detection component for the blood group antigens according to  claim 6 , wherein the reaction membrane is a nitrocellulose membrane having a back support, having a capillary flow rate of less than 90 seconds, and a width of 4 cm. 
     
     
         8 . A detection kit for blood group antigens, comprising:
 a test strip comprising a base board, wherein:
 a reaction membrane, a flushing pad, a water absorption pad, and a sealing pad are disposed on the base board, 
 the reaction membrane is disposed on a middle portion of the base board, 
 the flushing pad is disposed on a side of the reaction membrane, 
 one end of the flushing pad overlaps the reaction membrane, 
 the water absorption pad is disposed on another side of the reaction membrane, 
 one end of the water absorption pad overlaps another end of the reaction membrane, 
 one part of the sealing pad is stuck to the reaction membrane, 
 another part of sealing pad is stuck to the flushing pad, 
 a section of the reaction membrane disposed between one end of the sealing pad and the one end of the water absorption pad defines a testing section, 
 an A testing point, a B testing point, a D testing point, and a Ctl testing point are disposed on the testing section, 
 the A testing point is coated with a solidified anti-A monoclonal antibody, 
 the B testing point is coated with a solidified anti-B monoclonal antibody, 
 the D testing point is coated with a solidified anti-D monoclonal antibody, 
 the Ctl testing point is coated with a solidified anti-RBC monoclonal antibody; and 
   a sample addition device comprising a sampling ring, wherein the sampling ring is configure to add a sample to the A testing point, the B testing point, the D testing point, and the Ctl testing point at a same time.   
     
     
         9 . The detection kit for the blood group antigens according to  claim 8 , wherein the sampling ring is disposed with a handle and a plurality of protrusions equally spaced and configured to respectively correspond to the A testing point, the B testing point, the D testing point, and the Ctl testing point on the reaction membrane. 
     
     
         10 . The detection kit for the blood group antigens according to  claim 8 , wherein a tag is disposed on the water absorption pad. 
     
     
         11 . The detection kit for the blood group antigens according to  claim 8 , wherein the A testing point, the B testing point, the D testing point, and the Ctl testing point are linearly arrayed or staggered up and down to be shaped as an “N” along a width direction of the testing section. 
     
     
         12 . The detection kit for the blood group antigens according to  claim 9 , wherein a tag is disposed on the water absorption pad. 
     
     
         13 . The detection kit for the blood group antigens according to  claim 9 , wherein the A testing point, the B testing point, the D testing point, and the Ctl testing point are linearly arrayed or staggered up and down to be shaped as an “N” along a width direction of the testing section.