Novel glucose oxidase compositions

Abstract

Glucose oxidase and further compositions are described. The glucose oxidase compositions have glucose oxidase and at least one ingredient selected from one or more of carbohydrate (e.g. dietary fibre or saccharide), polyol or sugar alcohol while further compositions are sweeteners, food, compositions for fortification of food, nutraceuticals and pharmaceuticals. The compositions incorporate low glycemic index nutritive compound of glycemic index less than 70, preferably less than 50, and are in the form of powders, granules, crystalline compositions, flours, pills, tablets, capsules, pellets, powder for oral suspensions, gels, liquid solutions and suspensions, and sterile preparations. The compositions regulate one or more disease/conditions, including but not limited to those associated with blood, kidney, thyroid, nerves, joints, weight, diabetes, oxidative stress, cardiovascular disease, insulin resistance, amyloid foot ulcers, cataract, glaucoma, hypertension, metabolic disorders, digestive disorders, polycystic ovarian syndrome, mastopathy, dupuytren's contracture, gingivitis, periodontitis, dental caries, mouth disorders, cognitive dysfunction, and Parkinson's disease.

1 . Glucose oxidase compositions comprising glucose oxidase from 0.01-95% and at least one more ingredient in an amount of at least 5% selected from the group of carbohydrate, polyol and sugar alcohol. 
     
     
         2 . Glucose oxidase compositions as in  claim 1  wherein said at least one more ingredient comprises at least one low glycemic index nutritive ingredient with a glycemic index of less than 70. 
     
     
         3 . Glucose oxidase compositions as in  claim 2  wherein said one low glycemic index nutritive ingredient has a glycemic index of less than 50. 
     
     
         4 . Glucose oxidase compositions as in  claim 1  wherein the said at least one more ingredient is a polyol or a sugar alcohol selected from one or more of mannitol, xylitol, maltitol, isomalt, inositol, erythritol, lactitol, glycerol (glycerine), sorbitol, arabitol, ribitol, polyglycetol, hydrogenated starch hydrolysates and its derivatives (HSH), threitol, fruitol, iditol, volemitol, lactitol, galactitol, palatinose, palatinit, propylene glycol, reduced isomalto-oligosaccharides, fructooligosaccharide, maltooligosaccharide, reduced xylo-oligosaccharides, reduced gentiooligosaccharides, reduced maltose syrup, reduced glucose syrup, reduced starch sugar and combinations thereof. 
     
     
         5 . Glucose oxidase compositions as in  claim 1  wherein the said at least one more ingredient is a carbohydrate. 
     
     
         6 . Glucose oxidase compositions as in  claim 5  wherein the said carbohydrate is selected from a dietary fibre or a saccharide or combinations thereof. 
     
     
         7 . Glucose oxidase compositions as in  claim 6  wherein said saccharide is selected from one or more of tagatose, rhamnose, dextrins including cyclodextrin such as α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin), maltodextrin (including resistant maltodextrins such as Dietary fibersol-2™), dextran, ribulose, threose, arabinose, xylose, lyxose, allose, altrose, mannose, idose, levulose, maltose, invert sugar, isotrehalose, neotrehalose, palatinose or isomaltulose, erythrose, deoxyribose, gulose, idose, talose, erythrulose, xylulose, psicose, turanose, cellulose, cellobiose, starch, pectins such as amylopectin, glucosamine, mannosamine, fucose, glucuronic acid, gluconic acid, glucono-lactone, glycogen, abequose, galactosamine, beet oligosaccharides, isomalto-oligosaccharides (isomaltose, isomaltotriose, panose and the like), xylo-oligosaccharides (xylotriose, xylobiose and the like), gentio-oligoscaccharides (gentiobiose, gentiotriose, gentiotetraose and the like), sorbose, nigero-oligosaccharides, palatinose oligosaccharides, fucose, fructo-oligosaccharides (kestose, nystose and the like), maltotetraol, maltotriol, malto-oligosaccharides (maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose and the like), lactulose, melibiose, raffinose, rhamnose, ribose, isomerized liquid sugars such as high fructose corn/starch syrup (e.g., HFCS55, HFCS42, or HFCS90), coupling sugars, soybean oligosaccharides, and glucose syrup. 
     
     
         8 . Glucose oxidase compositions as in  claim 6  wherein said dietary fibre is one or more of non-starch polysaccharides, methylcellulose, β-glucans, mucilage, waxes, cyclodextrins, celluloses, hemicelluloses, starches, dextrins, inulins, lignins, chitins, pectins, beta-glucans (from oat bran, whole oats, oatrim or rolled oats, whole grain or dry-milled barley), fibres extracted from legumes, Chitosan, natural gums, xanthan gum, guar gum, gellan gum, tara gum, gum acacia, gum arabic, beta-mannan, locust bean gum (carob), psylium seed husk gum, galactomannan, glucomannan or konjac, karaya, tragacanth, hexoses, pentoses, resistant starch, plant waxes, alginic acids (alginates), natriumalginat, kaliumalginat, ammoniumalginat, calciumalginat, propylenglycolalginat, (PGA), agar, carrageen, raffinose, xylose, polydextrose, lactulose, vegetable gums, polysaccharide, heterosaccharides, agar-agar, alginate, oligosaccharides, arabinoxylan (a hemicellulose), fructooligosaccharides (FOS, oligofructose), galacto-oligosaccharides (GOS), human milk oligosaccharides (HMO), isomalto-oligosaccharides (IMO), lactosucrose, mannan-oligosaccharides (MOS), raffinose, stachyose, verbascose and derivatives thereof. 
     
     
         9 . Further compositions comprising Glucose oxidase compositions as in  claim 1  and additional ingredients wherein said further compositions are sweetener compositions, food compositions, compositions for fortification of food, nutraceutical compositions or pharmaceutical compositions. 
     
     
         10 . A sweetener composition as in  claim 9  comprising at least 0.01% of glucose oxidase, and at least 10% of one sweetening agent. 
     
     
         11 . Sweetener composition as in  claim 10  further comprising a dietary fibre. 
     
     
         12 . Sweetener composition as in  claim 11  comprising at least 0.01% of glucose oxidase, at least 0.1% dietary fibre and up to 99% of the sweetening agent. 
     
     
         13 . Sweetener composition as in  claim 11  comprising from 0.1-40% glucose oxidase, from 0.1%-89% dietary fibre and at least 10% of the sweetening agent. 
     
     
         14 . Sweetener composition as in  claim 11  comprising from 0.1-10% glucose oxidase, from 0.1%-70% dietary fibre and at least 10% of the sweetening agent. 
     
     
         15 . Sweetener composition as in  claim 11  comprising from 0.1-10% glucose oxidase, from 1%-30% dietary fibre and at least 10% of the sweetening agent. 
     
     
         16 . Sweetener composition as in  claim 10  wherein the sweetening agent is one or more of mannitol, xylitol, maltitol, isomalt, erythritol, lactitol, glycerol, sorbitol, polyglycetol, hydrogenated starch hydrolysates (HSH) and derivatives thereof, fructose, galactose, sucrose, lactose, tagatose, high fructose corn syrup (HFCS)-42, HFCS-55, HFCS-90, caramel, golden syrup, inverted sugar, refiners syrup, maple syrup, honey, sorghum, syrup, cane juice, barley malt syrup, coconut palm sugar, brown rice syrup, agave syrup and derivatives thereof. 
     
     
         17 . Food or nutraceutical compositions comprising sweetener compositions according to  claim 10 . 
     
     
         18 . A nutraceutical composition as in  claim 9  comprising as an additional ingredient one or more of dietary fibre, digestive enzymes, antioxidants, nucleotides or nucleic acids, vitamins, minerals, essential amino acids, non-essential amino acids, branched chain amino acids (BCAA), speciality nutraceuticals selected from one or more of glutathione, Human growth hormone (HGH), S Adenosyl L-Methionine (SAMe), D-Ribose, N-acetyl cysteine, and one or more plant extract. 
     
     
         19 . The nutraceutical composition as in  claim 18  wherein said plant extract is one or more of pine bark extract,  Costus igneus  (Insulin Plant) extract, bilberry extract, cranberry extract, blueberry extract, Gooseberry Extract, Rosemary Extract, Gymnema Sylvestra, mangosteen extract, pineapple extract, and kiwi extract and combinations thereof. 
     
     
         20 . The nutraceutical composition as in  claim 18  wherein said enzyme is one or more of papain, bromelain, Lipase, amylase, glucoamylase, beta glucanase, Fructosyl Transferase and combinations thereof. 
     
     
         21 . A food composition as in  claim 9  comprising glucose oxidase and one or more of the dietary fibres selected from the group consisting of non-starch polysaccharides, methylcellulose, β-glucans, mucilage, waxes, cyclodextrins, celluloses, hemicelluloses, starches, dextrins, inulins, lignins, chitins, pectins, beta-glucans (from oat bran, whole oats, oatrim or rolled oats, whole grain or dry-milled barley), fibres extracted from legumes, Chitosan, natural gums, xanthan gum, guar gum, gellan gum, tara gum, gum acacia, gum arabic, beta-mannan, locust bean gum (carob), psylium seed husk gum, galactomannan, glucomannan or konjac, karaya, tragacanth, hexoses, pentoses, resistant starch, plant waxes, alginic acids (alginates), natriumalginat, kaliumalginat, ammoniumalginat, calciumalginat, propylenglycolalginat, (PGA), agar, carrageen, raffinose, xylose, polydextrose, lactulose, vegetable gums, polysaccharide, heterosaccharides, agar-agar, alginate, oligosaccharides, arabinoxylan (a hemicellulose), fructooligosaccharides (FOS, oligofructose), galacto-oligosaccharides (GOS), human milk oligosaccharides (HMO), isomalto-oligosaccharides (IMO), lactosucrose, mannan-oligosaccharides (MOS), raffinose, stachyose, verbascose and derivatives thereof. 
     
     
         22 . The food composition as in  claim 21  wherein the said food composition is in the form of biscuits, snacks, confectionaries, bakery products, beverages, juices, products made from fruits or vegetables, sauces, jams, jellies, ready to eat foods, ready to cook foods, sweets and deserts. 
     
     
         23 . A pharmaceutical composition as in  claim 9  comprising a pharmaceutically active ingredient, glucose oxidase and a dietary fibre. 
     
     
         24 . The pharmaceutical composition as claimed in  claim 23  comprising pharmaceutically active ingredient comprising one or more of anti-diabetics, anti-hypertensives and medicines used for treatment of elevated total cholesterol, LDL, triglycerides, and medicines used to elevate HDL cholesterol, and also medicines for Alzheimer's symptoms and Parkinson's disease. 
     
     
         25 . The pharmaceutical composition as in  claim 23  wherein at least one dietary fibre is one or more of non-starch polysaccharides, methylcellulose, β-glucans, mucilage, waxes, cyclodextrins, celluloses, hemicelluloses, starches, dextrins, inulins, lignins, chitins, pectins, beta-glucans (from oat bran, whole oats, oatrim or rolled oats, whole grain or dry-milled barley), fibres extracted from legumes, Chitosan, natural gums, xanthan gum, guar gum, gellan gum, tara gum, gum acacia, gum arabic, beta-mannan, locust bean gum (carob), psylium seed husk gum, galactomannan, glucomannan or konjac, karaya, tragacanth, hexoses, pentoses, resistant starch, plant waxes, alginic acids (alginates), natriumalginat, kaliumalginat, ammoniumalginat, calciumalginat, propylenglycolalginat, (PGA), agar, carrageen, raffinose, xylose, polydextrose, lactulose, vegetable gums, polysaccharide, heterosaccharides, agar-agar, alginate, oligosaccharides, arabinoxylan (a hemicellulose), fructooligosaccharides (FOS, oligofructose), galacto-oligosaccharides (GOS), human milk oligosaccharides (HMO), isomalto-oligosaccharides (IMO), lactosucrose, mannan-oligosaccharides (MOS), raffinose, stachyose, verbascose and derivatives thereof. 
     
     
         26 . The pharmaceutical composition as in  claim 25  wherein dietary fibre is one or more of inulin, pectin, beta glucan and galactomannan. 
     
     
         27 . The pharmaceutical composition of  claim 23  wherein the composition is effective for controlling one or more of hyperglycemia, hypoglycemia, weight management challenges, obesity, oxidative stress, hyperuricemia, ketoacidosis, nonketotic hyperosmolar coma, cardiovascular disease, heart stroke, nephropathy, microalbuminuria, insulin resistance, amyloid foot ulcers, diabetic retinopathy, nonproliferative diabetic retinopathy, proliferative diabetic retinopathy, diabetic macular edema, cataract, glaucoma, High blood cholesterol (HDL) and LDL, triglycerides, atherosclerosis, hypertension, metabolic disorders, constipation, inflammatory bowel disease, ulcerative colitis, Crohn's disease, diverticulitis, neuropathy, coeliac disease, hypothyroidism, hyperthyroidism, polycystic ovarian syndrome, diabetes insipidus, necrobiosis lipoidica diabeticorum, mastopathy, limited joint mobility, frozen shoulder, dupuytren's contracture, trigger finger, carpal tunnel syndrome, gingivitis, periodontitis, dental caries, xerostomia, oral thrush, cognitive dysfunction, Alzheimer's symptoms and Parkinson's disease. 
     
     
         28 . Further compositions of  claim 9  wherein said further compositions are in the form of Powder composition, granule compositions, crystalline compositions, pellet, pill, tablet, hard and soft capsule compositions, powder for oral suspensions, elixirs, gels, liquid solutions and suspensions and sterile preparation. 
     
     
         29 . Further compositions as in  claim 28  in the form of capsule. 
     
     
         30 . Further compositions as in  claim 28  in the form of sachet. 
     
     
         31 . Multi-component kit comprising glucose oxidase as one component and dietary fibre as another component wherein the contents of the said components are combined with each other or with third ingredient before consumption. 
     
     
         32 . Multi-component kit comprising glucose oxidase as one component and dietary fibre as another component wherein the contents of the said components are consumed at an interval of not more than 12 hrs. 
     
     
         33 . The method according to  claim 32 , wherein the contents of the said components are consumed at an interval of not more than 6 hrs. 
     
     
         34 . Method of maintaining one or more of energy levels, body weight, blood sugar in a healthy individual by administering one or more compositions of  claim 9 . 
     
     
         35 . The method according to  claim 34 , wherein the compositions are administered 1-2 times a day. 
     
     
         36 . Method of treating an individual suffering from one or more of hyperglycemia, hypoglycemia, weight management challenges, obesity, oxidative stress, hyperuricemia, ketoacidosis, non-ketotic hyperosmolar coma, cardiovascular disease, heart stroke, nephropathy, microalbuminuria, insulin resistance, amyloid foot ulcers, diabetic retinopathy, non-proliferative diabetic retinopathy, proliferative diabetic retinopathy, diabetic macular edema, cataract, glaucoma, High blood cholesterol (HDL) and LDL, triglycerides, atherosclerosis, hypertension, metabolic disorders, constipation, inflammatory bowel disease, ulcerative colitis, Crohn's disease, diverticulitis, neuropathy, coeliac disease, hypothyroidism, hyperthyroidism, polycystic ovarian syndrome, diabetes insipidus, necrobiosis lipoidica diabeticorum, mastopathy, limited joint mobility, frozen shoulder, dupuytren's contracture, trigger finger, carpal tunnel syndrome, gingivitis, periodontitis, dental caries, xerostomia, oral thrush, cognitive dysfunction, Alzheimer's and Parkinson's disease by administering one or more compositions of  claim 9 . 
     
     
         37 . The method according to  claim 36 , wherein the compositions are administered 2-4 times a day. 
     
     
         38 . Method of regulating one or more of blood sugar level, hypertension, total cholesterol, LDL cholesterol, triglyceride, Alzheimer's symptoms and Parkinson's disease by consuming a sweetener composition according to  claim 10 . 
     
     
         39 . The method of  claim 36 , wherein from around 5-100 gm of the sweetener composition is consumed per day. 
     
     
         40 . The pharmaceutical composition as claimed in  claim 23  comprising pharmaceutically active ingredient comprising one or more of anti-diabetics, anti-hypertensives and medicines used for treatment of elevated total cholesterol, LDL, triglycerides, and medicines used to elevate HDL cholesterol, and also medicines for Alzheimer's symptoms and Parkinson's disease. 
     
     
         41 . Modified sugar comprising a sweetener composition according to  claim 10 . 
     
     
         42 . Modified sugar of  claim 41  comprising at least 50% of the sweetening agent.