Biomarkers for the Diagnosis of Lung Cancers

Abstract

Embodiments of the invention include a system and method of using biomarkers in the diagnosis of lung cancer. A subject can be screened for lung cancer based on expression of specific mRNAs, miRNAs or the detection of a nucleic acid, protein, peptide or other biological molecule in blood, serum or plasma. Embodiments include 29 specific miRNAs for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer, along with 81 additional nucleic acids, proteins or peptides for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer. Levels of more than one of the mRNAs, miRNAs or proteins can be scored and compared to one or more threshold values to diagnose or determine the prognosis of lung cancer. Embodiments also include a kit for screening healthy subjects from subjects affected with the disease.

1 . A method of diagnosing a lung cancer or determining a prognosis of a subject with a lung cancer, comprising the steps of:
 a) measuring the expression level of at least one biomarker in a test sample from the subject,   b) receiving the expression level of the at least one biomarker in the test sample by a computer and   c) comparing the expression level of the at least one biomarker in the test sample to a level in a base sample for the same at least one nucleic acid, protein or peptide, and   d) receiving a result comparing the expression levels of the at least one biomarker in the test sample measured in a) and the base sample measured in c), and   e) diagnosing or determining the prognosis of lung cancer based on altered expression of least one biomarker in the test sample as compared to the base sample as determined in a computer.   f) treating the subject for lung cancer based on the diagnosis or prognosis.   
     
     
         2 . The method of  claim 1 , further comprising a step of identifying a subtype of lung cancer,
 wherein the subtype of lung cancer is squamous cell lung cancer, non-small cell lung cancer, large cell lung cancer, small cell lung cancer and/or adenocarcinoma lung cancer.   
     
     
         3 . The method of  claim 1 , wherein the at least one biomarker is comprised of hsa-miR-1204, hsa-miR-141-3p, hsa-miR-1827, hsa-miR-938, hsa-miR-125b-5p, hsa-miR-297, hsa-miR-10a-5p, hsa-miR-145-5p, hsa-miR-217, hsa-miR-3185, hsa-miR-21-5p, hsa-miR-363-3p, hsa-miR-631, hsa-miR-655, hsa-miR-1245b-5p, hsa-miR-369-3p, hsa-miR-875-3p, hsa-miR-105-5p, hsa-miR-1253, hsa-miR-1285-3p, hsa-miR-512-5p, hsa-miR-550b-3p, hsa-miR-571 and hsa-miR-935. 
     
     
         4 . The method of  claim 1 , wherein the at least one biomarker is comprised of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 and/or 24 biomarkers. 
     
     
         5 . The method of  claim 1 , wherein the at least one biomarker is comprised of no more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 and/or 24 biomarkers. 
     
     
         6 . The method of  claim 1 , wherein the at least one biomarker is comprised of mRNA encoded by the genes TCTN3, DENND1A, FOS, MFSD11, PRPS1L1, F13A1, KLHL24, SSRP1, DDX24, KIF1B, RRP7A, MICALL1, C9orf16, SEPHS1, DMAC2L, ITGA2B, PURA, PAFAH1B3, PDXK, ARAF, TBCD, UBA1, EED, PARVB, RCN2, PGAP3, REX1BD (aka 619orf60), MED27, PIK3IP1, YTHDF3, BHMT2, ASF1A, ANXA8, ETFA, NMT1, EPHB3, KIF3C, LOH11CR2A (aka VWASA), SLC48A1, MAPKAPK5-AS1, PLA2G4B, CALHM2, SENP5, SIDT2, R3HDM4, MARK4, SSH3, ATOH1, AXIN2, TAS2R13, PCDHB1, VWA7, TRIM49, CNTD2, TSHZ2, CDHR5, KIF26B, PADI4, TRIM36, LGI2, KCNMB4, TTTY14, ELAVL3, PAGE4, PER2, ZNF142, CD4, CCS, NELL2, RNF44, KLHL21, DNAJB12, CDC123, GNAI3, TRADD and/or THRA. 
     
     
         7 . The method of  claim 1 , wherein the at least one biomarker is comprised of Tectonic-3, DENN domain-containing protein 1A, Proto-oncogene c-Fos, UNC93-like protein MFSD11, Ribose-phosphate pyrophosphokinase 3, Coagulation factor XIII A chain, Kelch-like protein 24, FACT complex subunit SSRP1, ATP-dependent RNA helicase DDX24, Kinesin-like protein KIF1B, Ribosomal RNA-processing protein 7 homolog A, MICAL-like protein 1, UPF0184 protein C9orf16, Selenide, water dikinase 1, ATP synthase subunit s, mitochondrial, Integrin alpha-11b, Transcriptional activator protein Pur-alpha, Platelet-activating factor acetylhydrolase IB subunit gamma, Pyridoxal kinase, Serine/threonine-protein kinase A-Raf, Tubulin-specific chaperone D, Ubiquitin-like modifier-activating enzyme 1, Polycomb protein EED, Beta-parvin, Reticulocalbin-2, Post-GPI attachment to proteins factor 3, Required for excision 1-B domain-containing protein, Mediator of RNA polymerase II transcription subunit 27, Phosphoinositide-3-kinase-interacting protein 1, YTH domain-containing family protein 3, S-methylmethionine—homocysteine S-methyltransferase BHMT2, Histone chaperone ASF1A, Annexin A8, Electron transfer flavoprotein subunit alpha mitochondrial, Glycylpeptide N-tetradecanoyltransferase 1, Ephrin type-B receptor 3, Kinesin-like protein KIF3C, Loss of heterozygosity, 11, chromosomal region 2, gene A, isoform CRA_b, Heme transporter HRG1, Putative uncharacterized protein encoded by MAPKAPK5-AS1, Cytosolic phospholipase A2 beta, Calcium homeostasis modulator protein 2, Sentrin-specific protease 5, SID1 transmembrane family member 2, R3H domain-containing protein 4, MAP/microtubule affinity-regulating kinase 4, CYSRT1 gene, Protein phosphatase Slingshot homolog 3, Protein atonal homolog 1, Axin-2, Taste receptor type 2 member 13, Protocadherin beta-1, von Willebrand factor A domain-containing protein 7, Tripartite motif-containing protein 49, Cyclin N-terminal domain-containing protein 2, Teashirt homolog 2, hypothetical protein FLJ20251, Cadherin-related family member 5, hypothetical protein FLJ11457, Kinesin-like protein KIF26B, Protein-arginine deiminase type-4, E3 ubiquitin-protein ligase TRIM36, Leucine-rich repeat LGI family member 2, Calcium-activated potassium channel subunit beta-4, ELAV-like protein 3, P antigen family member 4, Period circadian protein homolog 2, Zinc finger protein 142 (Clone pHZ-49), isoform CRA_b, U47924 CD4 molecule, Copper chaperone for superoxide dismutase, Protein kinase C-binding protein NELL2, RING finger protein 44, Kelch-like protein 21, DnaJ homolog subfamily B member 12, Cell division cycle protein 123 homolog, Guanine nucleotide-binding protein G(i) subunit alpha, Tumor necrosis factor receptor type 1-associated DEATH domain protein and Thyroid hormone receptor alpha. 
     
     
         8 . The method of  claim 1 , wherein the at least one biomarker is comprised of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123. 124. 125, 126 and/or 127 different nucleic acids, proteins or peptides. 
     
     
         9 . The method of  claim 1 , wherein the at least one biomarker is comprised of no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123. 124. 125, 126 and/or 127 different nucleic acids, proteins or peptides. 
     
     
         10 .- 14 . (canceled) 
     
     
         15 . A method of diagnosing a lung cancer or determining a prognosis of a subject with a lung cancer, the method comprising the steps of:
 a) measuring expression levels of at least two biomarkers in a test sample from the subject,   b) receiving the expression levels with a computer,   c) compiling the expression levels to yield a score,   d) comparing the score to one or more threshold values to diagnose or determine the prognosis of lung cancer, and   e) treating the subject for lung cancer based on the diagnosis or prognosis.   
     
     
         16 . The method of  claim 15 , further comprising a step of identifying a subtype of lung cancer,
 wherein the subtype of lung cancer is squamous cell lung cancer, non-small cell lung cancer, large cell lung cancer, small cell lung cancer and/or adenocarcinoma lung cancer.   
     
     
         17 . The method of  claim 15 , wherein the at least two biomarkers are mRNAs encoded by the genes TCTN3, DENND1A, FOS, MFSD11, PRPS1L1, F13A1, KLHL24, SSRP1, DDX24, KIF1B, RRP7A, MICALL1, C9orf16, SEPHS1, DMAC2L, ITGA2B, PURA, PAFAH1B3, PDXK, ARAF, TBCD, UBA1, EED, PARVB, RCN2, PGAP3, REX1BD (aka 619orf60), MED27, PIK3IP1, YTHDF3, BHMT2, ASF1A, ANXA8, ETFA, NMT1, EPHB3, KIF3C, LOH11CR2A (aka VWASA), SLC48A1, MAPKAPK5-AS1, PLA2G4B, CALHM2, SENP5, SIDT2, R3HDM4, MARK4, SSH3, ATOH1, AXIN2, TAS2R13, PCDHB1, VWA7, TRIM49, CNTD2, TSHZ2, CDHR5, KIF26B, PADI4, TRIM36, LGI2, KCNMB4, TTTY14, ELAVL3, PAGE4, PER2, ZNF142, CD4, CCS, NELL2, RNF44, KLHL21, DNAJB12, CDC123, GNAI3, TRADD and/or THRA. 
     
     
         18 . The method of  claim 15 , wherein the at least two biomarkers are proteins comprised of Tectonic-3, DENN domain-containing protein 1A, Proto-oncogene c-Fos, UNC93-like protein MFSD11, Ribose-phosphate pyrophosphokinase 3, Coagulation factor XIII A chain, Kelch-like protein 24, FACT complex subunit SSRP1, ATP-dependent RNA helicase DDX24, Kinesin-like protein KIF1B, Ribosomal RNA-processing protein 7 homolog A, MICAL-like protein 1, UPF0184 protein C9orf16, Selenide, water dikinase 1, ATP synthase subunit s, mitochondrial, Integrin alpha-11b, Transcriptional activator protein Pur-alpha, Platelet-activating factor acetylhydrolase IB subunit gamma, Pyridoxal kinase, Serine/threonine-protein kinase A-Raf, Tubulin-specific chaperone D, Ubiquitin-like modifier-activating enzyme 1, Polycomb protein EED, Beta-parvin, Reticulocalbin-2, Post-GPI attachment to proteins factor 3, Required for excision 1-B domain-containing protein, Mediator of RNA polymerase II transcription subunit 27, Phosphoinositide-3-kinase-interacting protein 1, YTH domain-containing family protein 3, S-methylmethionine—homocysteine S-methyltransferase BHMT2, Histone chaperone ASF1A, Annexin A8, Electron transfer flavoprotein subunit alpha mitochondrial, Glycylpeptide N-tetradecanoyltransferase 1, Ephrin type-B receptor 3, Kinesin-like protein KIF3C, Loss of heterozygosity, 11, chromosomal region 2, gene A, isoform CRA_b, Heme transporter HRG1, Putative uncharacterized protein encoded by MAPKAPK5-AS1, Cytosolic phospholipase A2 beta, Calcium homeostasis modulator protein 2, Sentrin-specific protease 5, SID1 transmembrane family member 2, R3H domain-containing protein 4, MAP/microtubule affinity-regulating kinase 4, CYSRT1 gene, Protein phosphatase Slingshot homolog 3, Protein atonal homolog 1, Axin-2, Taste receptor type 2 member 13, Protocadherin beta-1, von Willebrand factor A domain-containing protein 7, Tripartite motif-containing protein 49, Cyclin N-terminal domain-containing protein 2, Teashirt homolog 2, hypothetical protein FLJ20251, Cadherin-related family member 5, hypothetical protein FLJ11457, Kinesin-like protein KIF26B, Protein-arginine deiminase type-4, E3 ubiquitin-protein ligase TRIM36, Leucine-rich repeat LGI family member 2, Calcium-activated potassium channel subunit beta-4, ELAV-like protein 3, P antigen family member 4, Period circadian protein homolog 2, Zinc finger protein 142 (Clone pHZ-49), isoform CRA_b, U47924 CD4 molecule, Copper chaperone for superoxide dismutase, Protein kinase C-binding protein NELL2, RING finger protein 44, Kelch-like protein 21, DnaJ homolog subfamily B member 12, Cell division cycle protein 123 homolog, Guanine nucleotide-binding protein G(i) subunit alpha, Tumor necrosis factor receptor type 1-associated DEATH domain protein and Thyroid hormone receptor alpha. 
     
     
         19 . The method of  claim 15 , wherein the at least two biomarkers are comprised of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123. 124. 125, 126 and/or 127 different nucleic acids, proteins or peptides. 
     
     
         20 . The method of  claim 15 , wherein the at least two biomarkers are comprised of no more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123. 124. 125, 126 and/or 127 different nucleic acids, proteins or peptides. 
     
     
         21 . The method of  claim 1 , wherein the at least biomarker comprises mRNAs encoded by genes selected from TCTN3, DENND1A, FOS, MFSD11, PRPS1L1, F13A1, KLHL24, SSRP1, DDX24, KIF1B, RRP7A, MICALL1, C9orf16, SEPHS1, ITGA2B, PURA, PAFAH1B3, PDXK, ARAF, TBCD, UBA1, EED, PARVB, RCN2, PGAP3, MED27, PIK3IP1, YTHDF3, BHMT2, ASF1A, ANXA8, ETFA, NMT1, EPHB3, KIF3C, LOH11CR2A (aka VWASA), SLC48A1, MAPKAPK5-AS1, PLA2G4B, CALHM2, SENP5, SIDT2, R3HDM4, MARK4, SSH3, ATOH1, AXIN2, TAS2R13, PCDHB1, VWA7, TRIM49, CNTD2, TSHZ2, CDHR5, KIF26B, PADI4, TRIM36, LGI2, KCNMB4, TTTY14, ELAVL3, PAGE4, PER2, ZNF142, CD4, CCS, NELL2, RNF44, KLHL21, DNAJB12, CDC123, GNAI3, TRADD and THRA. 
     
     
         22 . The method of  claim 1 , wherein the at least biomarker comprises miRNAs selected from hsa-miR-1204, hsa-miR-141-3p, hsa-miR-1827, hsa-miR-938, hsa-miR-125b-5p, hsa-miR-297, hsa-miR-10a-5p, hsa-miR-145-5p, hsa-miR-217, hsa-miR-3185, hsa-miR-21-5p, hsa-miR-363-3p, hsa-miR-631, hsa-miR-655, hsa-miR-1245b-5p, hsa-miR-369-3p, hsa-miR-875-3p, hsa-miR-105-5p, hsa-miR-1253, hsa-miR-1285-3p, hsa-miR-512-5p, hsa-miR-550b-3p, hsa-miR-571 and hsa-miR-935. 
     
     
         23 . The method of  claim 1 , wherein the at least biomarker comprises proteins, selected from Tectonic-3, DENN domain-containing protein 1A, Proto-oncogene c-Fos, UNC93-like protein MFSD11, Ribose-phosphate pyrophosphokinase 3, Coagulation factor XIII A chain, Kelch-like protein 24, FACT complex subunit SSRP1, ATP-dependent RNA helicase DDX24, Kinesin-like protein KIF1B, Ribosomal RNA-processing protein 7 homolog A, MICAL-like protein 1, UPF0184 protein C9orf16, Selenide, water dikinase 1, ATP synthase subunit s, mitochondrial, Integrin alpha-11b, Transcriptional activator protein Pur-alpha, Platelet-activating factor acetylhydrolase IB subunit gamma, Pyridoxal kinase, Serine/threonine-protein kinase A-Raf, Tubulin-specific chaperone D, Ubiquitin-like modifier-activating enzyme 1, Polycomb protein EED, Beta-parvin, Reticulocalbin-2, Post-GPI attachment to proteins factor 3, Required for excision 1-B domain-containing protein, Mediator of RNA polymerase II transcription subunit 27, Phosphoinositide-3-kinase-interacting protein 1, YTH domain-containing family protein 3, S-methylmethionine—homocysteine S-methyltransferase BHMT2, Histone chaperone ASF1A, Annexin A8, Electron transfer flavoprotein subunit alpha mitochondrial, Glycylpeptide N-tetradecanoyltransferase 1, Ephrin type-B receptor 3, Kinesin-like protein KIF3C, Loss of heterozygosity, 11, chromosomal region 2, gene A, isoform CRA_b, Heme transporter HRG1, Putative uncharacterized protein encoded by MAPKAPK5-AS1, Cytosolic phospholipase A2 beta, Calcium homeostasis modulator protein 2, Sentrin-specific protease 5, SID1 transmembrane family member 2, R3H domain-containing protein 4, MAP/microtubule affinity-regulating kinase 4, CYSRT1 gene, Protein phosphatase Slingshot homolog 3, Protein atonal homolog 1, Axin-2, Taste receptor type 2 member 13, Protocadherin beta-1, von Willebrand factor A domain-containing protein 7, Tripartite motif-containing protein 49, Cyclin N-terminal domain-containing protein 2, Teashirt homolog 2, hypothetical protein FLJ20251, Cadherin-related family member 5, hypothetical protein FLJ11457, Kinesin-like protein KIF26B, Protein-arginine deiminase type-4, E3 ubiquitin-protein ligase TRIM36, Leucine-rich repeat LGI family member 2, Calcium-activated potassium channel subunit beta-4, ELAV-like protein 3, P antigen family member 4, Period circadian protein homolog 2, Zinc finger protein 142 (Clone pHZ-49), isoform CRA_b, U47924 CD4 molecule, Copper chaperone for superoxide dismutase, Protein kinase C-binding protein NELL2, RING finger protein 44, Kelch-like protein 21, DnaJ homolog subfamily B member 12, Cell division cycle protein 123 homolog, Guanine nucleotide-binding protein G(i) subunit alpha, Tumor necrosis factor receptor type 1-associated DEATH domain protein and Thyroid hormone receptor alpha. 
     
     
         24 . The method of  claim 15 , wherein the at least two biomarkers comprise mRNAs encoded by genes selected from TCTN3, DENND1A, FOS, MFSD11, PRPS1L1, F13A1, KLHL24, SSRP1, DDX24, KIF1B, RRP7A, MICALL1, C9orf16, SEPHS1, ITGA2B, PURA, PAFAH1B3, PDXK, ARAF, TBCD, UBA1, EED, PARVB, RCN2, PGAP3, MED27, PIK3IP1, YTHDF3, BHMT2, ASF1A, ANXA8, ETFA, NMT1, EPHB3, KIF3C, LOH11CR2A (aka VWA5A), SLC48A1, MAPKAPK5-AS1, PLA2G4B, CALHM2, SENP5, SIDT2, R3HDM4, MARK4, SSH3, ATOH1, AXIN2, TAS2R13, PCDHB1, VWA7, TRIM49, CNTD2, TSHZ2, CDHR5, KIF26B, PADI4, TRIM36, LGI2, KCNMB4, TTTY14, ELAVL3, PAGE4, PER2, ZNF142, CD4, CCS, NELL2, RNF44, KLHL21, DNAJB12, CDC123, GNAI3, TRADD and THRA. 
     
     
         25 . The method of  claim 15 , wherein the at least two biomarkers comprise miRNA selected from hsa-miR-1204, hsa-miR-141-3p, hsa-miR-1827, hsa-miR-938, hsa-miR-125b-5p, hsa-miR-297, hsa-miR-10a-5p, hsa-miR-145-5p, hsa-miR-217, hsa-miR-3185, hsa-miR-21-5p, hsa-miR-363-3p, hsa-miR-631, hsa-miR-655, hsa-miR-1245b-5p, hsa-miR-369-3p, hsa-miR-875-3p, hsa-miR-105-5p, hsa-miR-1253, hsa-miR-1285-3p, hsa-miR-512-5p, hsa-miR-550b-3p, hsa-miR-571 and hsa-miR-935. 
     
     
         26 . The method of  claim 15 , wherein the at least biomarker comprises proteins selected from Tectonic-3, DENN domain-containing protein 1A, Proto-oncogene c-Fos, UNC93-like protein MFSD11, Ribose-phosphate pyrophosphokinase 3, Coagulation factor XIII A chain, Kelch-like protein 24, FACT complex subunit SSRP1, ATP-dependent RNA helicase DDX24, Kinesin-like protein KIF1B, Ribosomal RNA-processing protein 7 homolog A, MICAL-like protein 1, UPF0184 protein C9orf16, Selenide, water dikinase 1, ATP synthase subunit s, mitochondrial, Integrin alpha-11b, Transcriptional activator protein Pur-alpha, Platelet-activating factor acetylhydrolase IB subunit gamma, Pyridoxal kinase, Serine/threonine-protein kinase A-Raf, Tubulin-specific chaperone D, Ubiquitin-like modifier-activating enzyme 1, Polycomb protein EED, Beta-parvin, Reticulocalbin-2, Post-GPI attachment to proteins factor 3, Required for excision 1-B domain-containing protein, Mediator of RNA polymerase II transcription subunit 27, Phosphoinositide-3-kinase-interacting protein 1, YTH domain-containing family protein 3, S-methylmethionine—homocysteine S-methyltransferase BHMT2, Histone chaperone ASF1A, Annexin A8, Electron transfer flavoprotein subunit alpha mitochondrial, Glycylpeptide N-tetradecanoyltransferase 1, Ephrin type-B receptor 3, Kinesin-like protein KIF3C, Loss of heterozygosity, 11, chromosomal region 2, gene A, isoform CRA_b, Heme transporter HRG1, Putative uncharacterized protein encoded by MAPKAPK5-AS1, Cytosolic phospholipase A2 beta, Calcium homeostasis modulator protein 2, Sentrin-specific protease 5, SID1 transmembrane family member 2, R3H domain-containing protein 4, MAP/microtubule affinity-regulating kinase 4, CYSRT1 gene, Protein phosphatase Slingshot homolog 3, Protein atonal homolog 1, Axin-2, Taste receptor type 2 member 13, Protocadherin beta-1, von Willebrand factor A domain-containing protein 7, Tripartite motif-containing protein 49, Cyclin N-terminal domain-containing protein 2, Teashirt homolog 2, hypothetical protein FLJ20251, Cadherin-related family member 5, hypothetical protein FLJ11457, Kinesin-like protein KIF26B, Protein-arginine deiminase type-4, E3 ubiquitin-protein ligase TRIM36, Leucine-rich repeat LGI family member 2, Calcium-activated potassium channel subunit beta-4, ELAV-like protein 3, P antigen family member 4, Period circadian protein homolog 2, Zinc finger protein 142 (Clone pHZ-49), isoform CRA_b, U47924 CD4 molecule, Copper chaperone for superoxide dismutase, Protein kinase C-binding protein NELL2, RING finger protein 44, Kelch-like protein 21, DnaJ homolog subfamily B member 12, Cell division cycle protein 123 homolog, Guanine nucleotide-binding protein G(i) subunit alpha, Tumor necrosis factor receptor type 1-associated DEATH domain protein and Thyroid hormone receptor alpha.