Heavy chain only antibodies to pdgf

Abstract

Disclosed herein are monospecific HCAb antibodies with antigen-binding specificity to PDGF and bispecific antibodies with antigen-binding specificities to PDGF-2 and VEGF or to PDGF and ANG-2.

1 . A heavy chain only antibody (HCAb) with an antigen-binding specificity for PDGF, wherein the HCAb has the variable heavy (VH) region sequence of one of SEQ ID NOs: 10, 14, 18, 22, 26, 30, 34, 38, 42, 46, or 50. 
     
     
         2 . (canceled) 
     
     
         3 . The HCAb of  claim 1 , wherein PDGF is PDGF-BB. 
     
     
         4 . The HCAb of  claim 1 , wherein the HCAB comprises one or more complementarity determining regions (CDR) selected from SEQ ID NOs: 11-13, 15-17, 19-21, 23-25, 27-29, 31-33, 35-37, 39-41, 43-45, 47-49 and 51-53. 
     
     
         5 . (canceled) 
     
     
         6 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:11-13. 
     
     
         7 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:15-17. 
     
     
         8 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:19-21. 
     
     
         9 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:23-25. 
     
     
         10 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:27-29. 
     
     
         11 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:31-33. 
     
     
         12 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:35-37. 
     
     
         13 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:39-41. 
     
     
         14 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:43-45. 
     
     
         15 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:47-49. 
     
     
         16 . The HCAb of  claim 4 , wherein the CDRs comprise SEQ ID NOs:51-53. 
     
     
         17 . The HCAb of  claim 1 , wherein at least one amino acid of the VH region is substituted, added, or deleted and the HCAb retains its specificity for PDGF-BB. 
     
     
         18 . An HCAb with an antigen-binding specificity for PDGF-BB, wherein the CDR1 comprises GFTFSSYA (SEQ ID NO:23), and wherein the amino acid at position 7 is substituted with any amino acid and the HCAb retains its specificity for PDGF-BB. 
     
     
         19 . The HCAb of  claim 18 , wherein the amino acid at position 7 is substituted with a conservative amino acid and the HCAb retains its specificity for PDGF-BB. 
     
     
         20 . The HCAb of  claim 18 , wherein the amino acid at position 7 is substituted with an amino acid of the same class and the HCAb retains its specificity for PDGF-BB.