Red blood cell targeted factor viii and method of using the same

Abstract

Targeted Factor VIII molecules comprising a Factor VIII linked with at least one domain that specifically binds to a membrane protein on a red blood cell is provided. The disclosed targeted coagulation factors prolong their duration of action and thus, are an improvement for the treatment of hematological diseases such as hemophilia A.

1 - 21 . (canceled) 
     
     
         22 . A recombinant fusion protein comprising a protein wherein extension of circulating half-life would be beneficial to a patient, and at least one binding domain that specifically binds to a membrane protein on a red blood cell. 
     
     
         23 . The recombinant fusion protein of  claim 22 , wherein the membrane protein is glycophorin A or Band3. 
     
     
         24 . The recombinant fusion protein of  claim 22 , wherein the binding domain is an antibody, an antibody fragment, a scFv, a peptide, a peptide mimetic, or a small molecule. 
     
     
         25 . The recombinant fusion protein of  claim 24 , wherein the binding domain is a scFv and the membrane protein is glycophorin A. 
     
     
         26 . The recombinant fusion protein of  claim 25 , wherein the scFv comprises a heavy chain selected from the group consisting of SEQ IS NOS: 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65 and 67. 
     
     
         27 . The recombinant fusion protein of  claim 25 , wherein the scFv comprises a light chain selected from the group consisting of SEQ IS NOS: 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64 and 66. 
     
     
         28 . The recombinant fusion protein of  claim 25 , wherein the scFv comprises a heavy chain selected from the group consisting of SEQ IS NOS: 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65 and 67, and a light chain selected from the group consisting of SEQ IS NOS: 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64 and 66.